Genotype Phenotype Coupling. Methods and Protocols

Front Matter ....Pages i-xii
 Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin  (Nandi Zhou, Rongfeng Cai, Xuyan Han)....Pages 1-18
 Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins  (Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou et al.)....Pages 19-41
 cDNA Display: A Stable and Simple Genotype–Phenotype Coupling Using a Cell-Free Translation System  (Hidenao Arai, Shigefumi Kumachi, Naoto Nemoto)....Pages 43-56
 cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution  (Tai Kubo, Mohammed Naimuddin)....Pages 57-77
 Rapid Antigen and Antibody-Like Molecule Discovery by Staphylococcal Surface Display  (Marco Cavallari)....Pages 79-94
 Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library  (Valentin Jakob, Saskia Helmsing, Michael Hust, Martin Empting)....Pages 95-113
 In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties  (John Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter et al.)....Pages 115-142
 Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens  (Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel et al.)....Pages 143-155
 Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning  (Kathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, David S. Peabody)....Pages 157-171
 Isolation of Antigen-Specific VHH Single-Domain Antibodies by Combining Animal Immunization with Yeast Surface Display  (Lukas Roth, Simon Krah, Janina Klemm, Ralf Günther, Lars Toleikis, Michael Busch et al.)....Pages 173-189
 Selection and Characterization of Anti-idiotypic Shark Antibody Domains  (Doreen Könning, Stefan Zielonka, Anna Kaempffe, Sebastian Jäger, Harald Kolmar, Christian Schröter)....Pages 191-209
 Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression  (Steffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, Harald Kolmar)....Pages 211-222
 Methods for Construction of Yeast Display Libraries of Four-Domain T-Cell Receptors  (Flávio Sádio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp)....Pages 223-248
 Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting  (Anna Kaempffe, Sebastian Jäger, Doreen Könning, Harald Kolmar, Christian Schröter)....Pages 249-266
 Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires  (Sebastian Jäger, Simon Krah, Doreen Könning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche et al.)....Pages 267-287
 Rapid Generation of Chicken Immune Libraries for Yeast Surface Display  (Jan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, Harald Kolmar)....Pages 289-302
 Ligand Engineering via Yeast Surface Display and Adherent Cell Panning  (Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel)....Pages 303-320
 Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping  (Carlos A. Cruz-Teran, Kaitlyn Bacon, Balaji M. Rao)....Pages 321-334
 Chemical Modification of the Yeast Cell Surface Allows the Switch Between Display and Soluble Secretion of Full-Length Antibodies  (Simon Krah, Ralf Günther, Stefan Becker, Stefan Zielonka, Laura Rhiel)....Pages 335-349
 Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Genotype–Phenotype Coupling Transposon Vectors  (Karen Berg, Vanessa Nicole Schäfer, Natalie Tschorn, Jörn Stitz)....Pages 351-361
 Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult-to-Drug Targets  (Zachary R. Crook, Gregory P. Sevilla, Andrew J. Mhyre, James M. Olson)....Pages 363-396
 Engineering Antibodies on the Surface of CHO Cells  (Annalee W. Nguyen, Kevin Le, Jennifer A. Maynard)....Pages 397-422
 Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies  (Juliet Rashidian, Joshua Lloyd)....Pages 423-441
Back Matter ....Pages 443-445