Gene Probes

Content: 
Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 1
Pages ix-xii

Preface
Page xiii
P. MICHAEL CONN

1 - Using the Xenopus Oocyte System for Expression and Cloning of Neuroreceptors and Channels
Pages 3-29
Leslie Kushner, Juan Lerma, Michael V.L. Bennett, R. Suzanne Zukin

2 - Expression of Neurotransmitter Receptors and Voltage-Activated Channels from Brain mRNA in Xenopus Oocytes
Pages 30-45
Katumi Sumikawa, Ian Parker, Ricardo Miledi

3 - Expression of Mammalian Plasma Membrane Receptors in Xenopus Oocytes: Studies of Thyrotropin-Releasing Hormone Action
Pages 46-61
Richard E. Straub, Yoram Oron, Marvin C. Gershengorn

4 - Expression of Exogenous Voltage-Gated Calcium Channels in Xenopus Oocytes
Pages 62-75
John P. Leonard

5 - Quantification of in Situ Hybridization Histochemistry for Analysis of Brain Function
Pages 79-97
Charles R. Gerfen

6 - In Situ Hybridization: A Methodological Guide
Pages 98-112,112a,113-126
Joseph T. McCabe, Donald W. Pfaff

7 - Combination of in Situ Hybridization with Immunohistochemistry and Retrograde Tract-Tracing
Pages 127-136
Alan G. Watts, Larry W. Swanson

8 - Localization of Neuronal mRNAs by Hybridization Histochemistry
Pages 136-150
Ruth E. Siegel

9 - In Situ Detection of Peptide Messenger RNA Using Complementary RNA Probes
Pages 150-164
A. Giaid, S.J. Gibson, J.H. Steel, J.M. Polak

10 - Quantification of mRNA in Discrete Cell Groups of Brain by in Situ Hybridization Histochemistry
Pages 164-182
Margaret A. Miller, Janice H. Urban, Daniel M. Dorsa

11 - In Situ Hybridization Approaches to Human Neurological Disease
Pages 183-196
Gerald A. Higgins, Vei H. Mah

12 - Localization of Peptide Gene Expression by in Situ Hybridization at Electron Microscopic Level
Pages 197-208
Georges Pelletier, Yiai Tong, Jacques Simard, Hui-Fen Zhao, Fernand Labrie

13 - In Situ Hybridization for Detecting Gonadotropin-Releasing Hormone Messenger RNA and Measuring Physiologically Stimulated Changes
Pages 208-222
J. Fielding Hejtmancik, P. Michael Conn, Donald W. Pfaff

14 - Location of Gene Expression in Tissue Sections by Hybridization Histochemistry Using Oligodeoxyribonucleotide Probes
Pages 222-238
Jennifer D. Penschow, Jim Haralambidis, Scott Pownall, John P. Coghlan

15 - In Situ mRNA Hybridization: Standard Procedures and Novel Approaches
Pages 238-281
C. Anthony Altar, Susan Ryan, Mary Abood, James H. Eberwine

16 - Nonradioactive in Situ Hybridization Histochemistry with Digoxigenin–Deoxyuridine 5′-Triphosphate-Labeled Oligonucleotides
Pages 282-292
Frank Baldino Jr., Michael E. Lewis

17 - Quantitation of Nuclear Low-Level Gene Expression in Central Nervous System Using Solution Hybridization and in Situ Hybridization
Pages 293-303
Mariann Blum, James L. Roberts

18 - Immunoscreening λgt11 cDNA Library
Pages 307-318
John S. Kizer, Thomas O. Brock

19 - Rapid Identification of DNA Clones: Utilization of Same Degenerate Oligonucleotides for Both Screening and Sequencing
Pages 319-328
R. Nichols, Jack E. Dixon

20 - Molecular Cloning of Nicotinic Acetylcholine Receptor Genes
Pages 328-363
Jim Boulter, Paul D. Gardner

21 - Lineage Analysis in the Vertebrate Nervous System by Retrovirus-Mediated Gene Transfer
Pages 367-392
Connie Cepko

22 - Use of Transgenic Models to Access Neural Lineages in Mammals
Pages 392-421
Kevin A. Kelley

23 - Characterization of Molecular Pathology of Alzheimer's Disease
Pages 425-443
Michel Goedert

Appendix I - Restriction Endonuclease Sites of Cleavage
Pages 447-450

Appendix II - Amino Acids and Genetic Code
Page 451

Index
Pages 453-464