Gene Manipulation and Expression

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Genomic Structure and Evolution of Bacillus Licheniformis ϑ and LP52 Phage Family....Pages 3-21
Expression of the Synthetic Proenkephalin Gene in E.coli ....Pages 22-32
Cloning and Expression of Bacillus β -Glucanase Genes....Pages 33-46
Regulation of α-Amylase Synthesis in Bacillus Subtilis ....Pages 47-61
New Variations on an Old Theme: Type I Restriction Enzymes and their Recognition Sequences....Pages 62-78
The Eco DXX1 Restriction and Modification System of Escherichia Coli ET7....Pages 79-94
Biological Function of Type I Restriction Enzymes....Pages 95-109
The Fosfomycin Resistance Transposon Tn2921....Pages 110-120
Transposition-Like Events Mediated by Single-Ended Derivatives of Transposon Tn21....Pages 121-132
Cloning and Expression of Determinants Encoding Toxigenicity in Enterobacteria....Pages 133-143
Extrachromosomal Replicons in Streptomyces Lavendulae-Grasserius ....Pages 144-152
Front Matter....Pages 153-153
Genetic Dissection of E.coli RNA Polymerase....Pages 155-172
Non-Essential Sequences in the β Subunit of E.Coli RNA Polymerase....Pages 173-181
Sequence Analysis of the β Gene of E.coli RNA Polymerase: Correlation with Structure-Function Studies....Pages 182-195
DNA-Dependent RNA Polymerase from Streptomyces Granaticolor ....Pages 196-208
Transcriptional and Translational Signals in Phages PZA and ø29....Pages 209-224
Host Vector System with the P R , Promoter of Phage Lambda....Pages 225-239
On the Difficulties of Defining and Measuring Promoter Strength; the Case of the Promoters of Bacterial rRNA Genes....Pages 240-252
Front Matter....Pages 253-253
Cyclic AMP in Bacteria: Catabolite Repression and Related Effects....Pages 255-272
DNA Sequences Involved in Expression and Regulation of deoR -, cytR - and cAMP/CRP-Controlled Genes in Escherichia Coli ....Pages 273-288
Front Matter....Pages 253-253
Differential Cellular Distribution of Cyclic AMP-Dependent Protein Kinase During Development of Dictyostelium Discoideum ....Pages 289-304
Amplification of the Adenylate Cyclase Gene in Escherichia Coli K12....Pages 305-317
Factors Regulating the Activity of Escherichia Coli Adenylate Cyclase....Pages 318-338
Regulation of Carbohydrate Metabolism by the Bacterial PEP:Sugar Phosphotransferase System....Pages 339-355
ATP-Dependent HPr Kinase Involved in Regulation of Carbohydrate Uptake in Gram-Positive Bacteria: Inducer Exclusion and Inducer Expulsion....Pages 356-366
Regulation of cAMP Synthesis in Streptomyces Granaticolor ....Pages 367-378
Differences in Regulation of a Bacillus Megaterium Metalloproteinase during Growth and Sporulation....Pages 379-391
Front Matter....Pages 391-391
Ribosomal Protein S1: “The Messenger RNA-Catching Arm” of Escherichia Coli Ribosome....Pages 393-406
Initiation of Translation of Genetic Information in Streptomyces Aureofaciens ....Pages 407-428
 Bacilli as Hosts for Protein Production....Pages 429-439
Structure and Expression of a Chloramphenicol Resistance Gene, cat-86 , Cloned From Bacillus Pumilus ....Pages 440-447
An Approach to Controlled Removal of the 3′ End of Ribosomal 16S RNA....Pages 448-454
Three Ribosomal tRNA-Binding Sites Involved in the Elongation Process....Pages 455-477
Structure, Function and Evolution of the 3′ Ends of Small Ribosomal Subunit RNA....Pages 478-496
Amino Acid Residues in Elongation Factor Tu from Escherichia Coli near the Binding Site for the 3′ Terminus of Aminoacyl-tRNA....Pages 497-508
Structural and Functional Alterations of Protein-Synthesis Elongation Factor Tu Purifted from Streptomyces Aureofactens in an Aggregated State....Pages 509-521
Excessive Translational Accuracy Can Inhibit Growth....Pages 522-535
Particulate Protein-Synthesis Factors Associated with Translatable mRNA in Mammalian Cells....Pages 536-552
Back Matter....Pages 553-559