FOXO Transcription Factors: Methods and Protocols

Front Matter ....Pages i-xii
 Introduction to FOXO Biology  (Wolfgang Link)....Pages 1-9
Front Matter ....Pages 11-11
 Characterization of MicroRNAs Regulating FOXO Expression  (Doug Hanniford, Eva Hernando)....Pages 13-28
 Identification of Transcription Factor-Binding Sites in the Mouse FOXO1 Promoter  (Christopher P. Cardozo)....Pages 29-40
 Investigating Mechanisms that Control Ubiquitin-Mediated DAF-16/FOXO Protein Turnover  (Thomas Heimbucher, Coleen T. Murphy)....Pages 41-49
 Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity  (Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, Guillermo Velasco)....Pages 51-59
 Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes  (María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, Yolanda Olmos)....Pages 61-76
 Characterization of FOXO Acetylation  (Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, Eric W-F Lam)....Pages 77-90
 Transcriptional Activity of FOXO Transcription Factors Measured by Luciferase Assays  (Antonia Ávila-Flores, Javier Arranz-Nicolás, Isabel Mérida)....Pages 91-102
 Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes  (Vanessa Henriques, Susana Machado, Wolfgang Link, Bibiana I. Ferreira)....Pages 103-113
 Using ChIP-Based Approaches to Characterize FOXO Recruitment to its Target Promoters  (Neeraj Kumar, Arnab Mukhopadhyay)....Pages 115-130
 RNAi-Mediated Silencing of FOXO Factors  (Priyankar Sanphui, Akash Saha, Subhas Chandra Biswas)....Pages 131-140
Front Matter ....Pages 141-141
 Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO  (Francisco Castillo, Thomas A. Mackenzie, Bastien Cautain)....Pages 143-149
 High-Throughput Image-Based Screening to Identify Chemical Compounds Capable of Activating FOXO  (Marta Barradas, Wolfgang Link, Diego Megias, Pablo J. Fernandez-Marcos)....Pages 151-161
 Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus  (Susana Machado, Catarina Raposo, Bibiana I. Ferreira, Wolfgang Link)....Pages 163-170
 Quantifying Tissue-Specific Overexpression of FOXO in Drosophila via mRNA Fluorescence In Situ Hybridization Using Branched DNA Probe Technology  (Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, Anthony Cammarato)....Pages 171-190
Front Matter ....Pages 191-191
 Genome-Wide Analysis for Identifying FOXO Protein-Binding Sites  (Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, Li Wang)....Pages 193-203
 Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors  (Bradford E. Peercy, Martin F. Schneider)....Pages 205-217
 Following Transcriptome to Uncover FOXO Biological Functions  (Raymond Liang, Vijay Menon, Saghi Ghaffari)....Pages 219-227
Front Matter ....Pages 229-229
 Hydra as Model to Determine the Role of FOXO in Longevity  (Thomas C. G. Bosch)....Pages 231-238
 Genetic Ablation of FOXO in Mice to Investigate Its Physiological Role  (Hwanhee Oh, Jihye Paik)....Pages 239-248
Front Matter ....Pages 249-249
 Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity  (Timothy A. Donlon, Philip M. C. Davy, Bradley J. Willcox)....Pages 251-257
 Analysis of FOXO3a Gene Polymorphism Associated with Asthma  (Tejas Shah, Kalpana Joshi)....Pages 259-266
Back Matter ....Pages 267-270