Fluorescence Microscopy and Fluorescent Probes

Front Matter....Pages i-xviii
Fluorescence Microscopy: State of the Art....Pages 1-14
Fluorescence Lifetime-Resolved Imaging Microscopy: A General Description of Lifetime-Resolved Imaging Measurements....Pages 15-33
Confocal Fluorescence Lifetime Imaging....Pages 35-46
Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens....Pages 47-56
Fluorescent Probes....Pages 57-60
Flow Cytometry versus Fluorescence Microscopy....Pages 61-66
Multichannel Fluorescence Microscopy and Digital Imaging - On the Exciting Developments in Fluorescence Microscopy....Pages 67-70
Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine....Pages 71-78
A Versatile Time-Resolved Laser Scanning Confocal Microscope....Pages 79-83
Disappearance of Cytoplasmic Ca 2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells....Pages 85-89
Distribution of Individual Cytoplasmic pH Values in a Cell Suspension....Pages 91-93
The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus ....Pages 95-99
On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red....Pages 101-106
Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes....Pages 107-112
The Effect of Protein Binding on the Calibration Curve of the pH Indicator BCECF....Pages 113-118
Artifacts in Fluorescence Ratio Imaging....Pages 119-123
Use of Fluorescent Probes and CLSM for pH-Monitoring in the Whole Plant Tissue. pH-Changes in the Shoot Apex of Chenopodium rubrum Related to Organogenesis....Pages 125-132
Spatial Resolution of Cortical Cerebral Blood Flow and Brain Intracellular pH as Measured by In Vivo Fluorescence Imaging....Pages 133-138
Is a Potential-Sensitive Probe diS-C 3 (3) a Nernstian Dye?: Time-Resolved Fluorescence Study with Liposomes as a Model System....Pages 139-143
Kinetic Behavior of Potential-Sensitive Fluorescent Redistribution Probes: Modelling of the Time Course of Cell Staining....Pages 145-149
Speed of Accumulation of the Membrane Potential Indicator diS-C 3 (3) in Yeast Cells....Pages 151-155
Spectral Effects of Slow Dye Binding to Cells and Their Role in Membrane Potential Measurements....Pages 157-162
Exploitation of Rhodamine B in the Killer Toxin Research....Pages 163-167
“In Situ” Estimates of the Spatial Resolution for “Practical” Fluorescence Microscopy of Cell Nuclei....Pages 169-173
Requirements for a Computer-Based System for Fish Applications....Pages 175-178
Fluorescent Dyes and Dye Labelled Probes for Detection of Nucleic Acid Sequences in Biological Material....Pages 179-183
Fluorescence in Situ Hybridization (FISH) in Cytogenetics of Leukemia....Pages 185-189
Estimation of “START” in Saccharomyces cerevisiae by Flow Cytometry and Fluorescent Staining of DNA and Cell Protein....Pages 191-195
Fluorescence Image Cytometry of DNA Content: A Comparative Study of Three Fluorochromes and Four Fixation Protocols....Pages 197-201
 In Vivo Tissue Characterization Using Environmentally Sensitive Fluorochromes....Pages 203-209
Sensitive and Rapid Detection of ß-Galactosidase Expression in Intact Cells by Microinjection of Fluorescent Substrate....Pages 211-215
Fluorogenic Substrates Reveal Genetic Differences in Aldehyde-Oxidating Enzyme Patterns in Rat Tissues....Pages 217-221
Binding of Prothrombin Fragment 1 to Phosphatidylserine Containing Vesicles: A Solvent Relaxation Study....Pages 223-227
New Thiol Active Fluorophores for Intracellular Thiols and Glutathione Measurement....Pages 229-233
Quantification of Macrophages in the Cardiovascular System of Hypercholesterolemic Rabbits by Use of Digital Image Processing....Pages 235-239
Fluorescence Assay for Studying P-Glycoprotein Function at Single Cell Level....Pages 241-245
Alterations of Vimentin-Nucleus Interactions as an Early Phase in Cholesterol Oxide - Induced Endothelial Cell Damage....Pages 247-251
Fluorescence Microscopy of Rye Cell Walls from Kernels to Incubated Doughs....Pages 253-255
Practical Approach for Immunohistochemical Staining of Muscle Biopsies....Pages 257-259
Rapid Automatic Segmentation of Fluorescent and Phase-Contrast Images of Bacteria....Pages 261-266
Use of Confocal Microscopy for Absolute Measurement of Cell Volume and Total Cell Surface Area....Pages 267-271
Cell Volume Measurements Using Confocal Laser Scanning Microscopy....Pages 273-277
Subcellular Cytofluorometry in Confocal Microscopy....Pages 279-283
Application of Confocal Microscopy to 3-D Reconstruction and Morphometrical Analysis of Capillaries....Pages 285-289
Retrieving Spatiotemporal Information from Confocal Data: A Study Using Melanotrope Cells of Xenopus Laevis ....Pages 291-295
Dynamics of Actin Measured by Fluorescence Correlation Microscopy (FCM)....Pages 297-301
Back Matter....Pages 303-306