Flow Cytometry: New Developments

Front Matter....Pages I-IX
Front Matter....Pages 1-1
 Cytosolic Events Related to Cell Activation: Potential Interaction between Signal and Energy Transduction Pathways in Smooth Muscle Cells....Pages 3-16
Studies of Changes in Cytoplasmic pH and Membrane Potential Using Flow Cytometry....Pages 17-27
Measurement of Enzyme Kinetics by Flow Cytometry....Pages 29-47
Endocytosis Analysis by Flow Cytometry : Study of the Endocytosis of Fluoresceinylated Neoglycoproteins Via Membrane Lectins of Macrophages....Pages 49-64
Analysis of Intracellular Antigens by Flow Cytometry: Methods for Cell Permeabilization to Antibodies ....Pages 65-101
Expression and Role of Surface Markers of Immunocompetent Cells in Non Lymphoid Systems....Pages 103-120
Change in Lipid Composition and in Membrane Fluidity of Human Peripheral Blood Lymphocytes Undergoing Blastic Transformation....Pages 121-129
Front Matter....Pages 131-131
Cellular Proliferation History by Fluorescent Analysis....Pages 133-139
Identification and Isolation of Bone Marrow Stem Cells....Pages 141-154
T Lymphocytes Recognition Molecules in Homing : A Flow Cytometry Study of Lectin-Glycoconjugates Interactions....Pages 155-164
Three- and Four-Color Immunofluorescence Analysis by Flow Cytometry....Pages 165-187
Energy Transfer....Pages 189-203
Fluid Mechanical Properties of Flow Cytometers and Assessment Cell-Cell Adhesion Forces....Pages 205-217
Use of biotin-labeled growth factors for receptor studies....Pages 219-225
 Cytometrie Tout Terrain or Bush DNA Cytometry....Pages 227-241
Front Matter....Pages 243-243
Bromodeoxuridine Procedures for Analysis of DNA Synthesis....Pages 245-258
Flow Cytogenetics: Fundamentals and New Developments....Pages 259-268
 In Situ Hybridization....Pages 269-276
The Molecular Basis of Mammalian Cell Growth Control....Pages 277-290
Flow Cytometric Analysis of Nuclear Associated Antigens....Pages 291-310
Front Matter....Pages 243-243
Chromatin Changes Accompanying Apoptosis Detected by Flow Cytometry....Pages 311-326
Gene Mapping and PCR Applications with Flow-Sorted Chromosomes....Pages 327-342
Cytometry, Antitumour Drugs and DNA Topoisomerases....Pages 343-354
Detection of Apoptosis using Fluorescent In Situ Nick Translation....Pages 355-362
Front Matter....Pages 363-363
Flow Cytometry Data Analysis. Basic Concepts and Analysis of Mono-Dimensional Immunofluorescence Histograms....Pages 365-393
Analysis of Multiparameter Flow Cytometric Data....Pages 395-411
Expert Assistance for Flow Immunophenotyping....Pages 413-420
DNA Content and Cell Cycle Analysis by Flow Cytometry in Clinical Samples : Applications in Cancer....Pages 421-432
Immunomagnetic Particles for Cell Isolation....Pages 433-450
Optical Trapping: Instrumentation and Biological Applications....Pages 451-467
Back Matter....Pages 469-478