Flow Cytometry and Cell Sorting

Front Matter....Pages I-X
Introduction....Pages 1-3
Front Matter....Pages 5-5
Operation of a Flow Cytometer....Pages 7-23
Front Matter....Pages 25-25
Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies....Pages 27-33
Immunofluorescence: Basic Considerations....Pages 34-46
Multicolor Immunofluorescence Analysis....Pages 47-52
Combined Intracellular and Surface Staining....Pages 53-58
Scatchard Analysis by Flow Cytometry....Pages 59-62
Front Matter....Pages 63-63
Preparation and Staining of Cells for High-Resolution DNA Analysis....Pages 65-68
Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression....Pages 69-74
High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique....Pages 75-85
Front Matter....Pages 87-87
Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium....Pages 89-99
Biochemical Parameters of Cell Function....Pages 100-120
Molecular Analysis of Transcriptional Control: The FACS-Galactosidase Assay....Pages 121-123
Ligand Acidification by Nonadherent Cells....Pages 124-129
Front Matter....Pages 131-131
Powerful Preselection....Pages 133-140
High Gradient Magnetic Cell Sorting....Pages 141-152
Setup of a Flow Sorter....Pages 153-158
Sorting of Rare Cells....Pages 159-167
Fluorescence-Activated Chromosome Sorting....Pages 168-176
Flow Cytometry and Sorting of Plant Chromosomes....Pages 177-188
Front Matter....Pages 131-131
Large Particle Sorting....Pages 189-204
Front Matter....Pages 205-205
Biological and Laser Safety....Pages 207-210
Back Matter....Pages 211-223