Expanded Bed Chromatography

Front Matter....Pages i-v
Expanded bed adsorption: elution in expanded bed mode....Pages 1-9
Monitoring, modeling, and control strategies for expanded-bed adsorption processes....Pages 11-31
Factors affecting dispersion in expanded bed chromatography....Pages 33-41
Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors....Pages 43-51
On-line monitoring of the purification of GST-(His) 6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed....Pages 53-67
The effect of column verticality on separation efficiency in expanded bed adsorption....Pages 69-75
Evaluation of the effect of in-bed sampling on expanded bed adsorption....Pages 77-83
EBA columns with a distribution system based on local stirring....Pages 85-91
Simplified and more robust EBA processes by elution in expanded bed mode....Pages 93-97
Cell/adsorbent interactions in expanded bed adsorption of proteins....Pages 99-109
Validation issues related to expanded bed technology....Pages 111-114
Highly dense beaded sorbents suitable for fluidized bed applications....Pages 115-120
Characterization of very dense mineral oxide—gel composites for fluidized-bed adsorption of biomolecules....Pages 121-129
Expanded bed adsorption on supermacroporous cross-linked cellulose matrix....Pages 131-138
Characterisation of STREAMLINE TM Phenyl....Pages 139-144
Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins....Pages 145-151
A simplified purification procedure of α-lactalbumin from milk using Ca 2+ -dependent adsorption in hydrophobic expanded bed chromatography....Pages 153-158
Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media....Pages 159-168
Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption....Pages 169-188
Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent....Pages 189-193
Purification of Recombinant Human Serum Albumin Efficient purification using STREAMLINE....Pages 195-200
Direct coupling of expanded bed adsorption with a downstream purification step....Pages 201-207
Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography....Pages 209-217
Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli ....Pages 219-228
On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyser....Pages 229-235
Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column....Pages 237-245
Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis....Pages 247-254
Flow injection analysis of intracellular β -galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification....Pages 255-267
Back Matter....Pages 269-271