Essentials of Chemical Biology: Structures and Dynamics of Biological Macromolecules In Vitro and In Vivo

Essentials of Chemical Biology
Contents
Preface
Glossary of Physical and Chemical Terms
About the Companion Website
1 The Structures of Biological Macromolecules and Lipid Assemblies
	1.1 General introduction
	1.2 Protein structures
	1.3 Carbohydrate structures
	1.4 Nucleic acid structures
	1.5 Macromolecular lipid assemblies
	1.6 Structural forces in biological macromolecules
2 Chemical and Biological Synthesis
	2.1 Introduction to synthesis in chemical biology
	2.2 Chemical synthesis of peptides and proteins
	2.3 Chemical synthesis of nucleic acids
	2.4 Chemical synthesis of oligosaccharides
	2.5 Chemical synthesis of lipids
	2.6 Biological synthesis of biological macromolecules
	2.7 Directed biological synthesis of proteins
	2.8 Biological syntheses of nucleic acids, oligosaccharides and lipids
3 Molecular Biology as a Toolset for Chemical Biology
	3.1 Key concepts in molecular biology
	3.2 Tools and techniques in molecular biology
	3.3 Cloning and identification of genes in DNA
	3.4 Integrating cloning and expression
	3.5 Site-directed mutagenesis
4 Electronic and Vibrational Spectroscopy
	4.1 Electronic and vibrational spectroscopy in chemical biology
	4.2 Ultraviolet-visible spectroscopy
	4.3 Circular dichroism spectroscopy
	4.4 Vibrational spectroscopy
	4.5 Fluorescence spectroscopy
	4.6 Probing metal centres by absorption spectroscopy
5 Magnetic Resonance
	5.1 Magnetic resonance in chemical biology
	5.2 Key principles of NMR
	5.3 Two-dimensional NMR
	5.4 Multidimensional NMR
	5.5 Biological macromolecule structural information
	5.6 Electron paramagnetic resonance spectroscopy: key principles
6 Diffraction and Microscopy
	6.1 Diffraction and microscopy in chemical biology
	6.2 Key principles of X-ray diffraction
	6.3 Structural information from X-ray diffraction
	6.4 Neutron diffraction
	6.5 Key principles of electron microscopy
	6.6 Key principles of scanning probe microscopy
7 Molecular Recognition and Binding
	7.1 Molecular recognition and binding in chemical biology
	7.2 Theoretical models of binding
	7.3 Analysing molecular recognition and binding
	7.4 Biological molecular recognition studies
8 Kinetics and Catalysis
	8.1 Catalysis in chemical biology
	8.2 Steady state kinetic schemes
	8.3 Pre-steady-state kinetics
	8.4 Theories of biocatalysis
	8.5 Electron transfer
9 Mass Spectrometry and Proteomics
	9.1 Mass spectrometry in chemical biology
	9.2 Key principles in mass spectrometry
	9.3 Structural analysis of biological macromolecules and lipids by mass spectrometry
	9.4 The challenge of proteomics
	9.5 Genomics: assigning function to genes and proteins
10 Molecular Selection and Evolution
	10.1 Chemical biology and the origins of life
	10.2 Molecular breeding: natural selection acting on self-organisation
	10.3 Directed evolution of protein function
	10.4 Directed evolution of nucleic acids
	10.5 Catalytic antibodies
11 Chemical Biology of Cells
	11.1 General introduction
	11.2 Array technologies, microfluidics and miniaturisation
	11.3 Chemical genetics and potential new therapeutics
	11.4 Chemical cellular dynamics
	11.5 Chemical biology and in vivo cell connectomics
12 Chemical Biology of Stem Cells to Tissue Engineering
	12.1 General introduction
	12.2 Chemical stem cell biology
	12.3 The road to cell therapies
	12.4 Tissue engineering
13 Chemical Biology, Nanomedicine and Advanced Therapeutics
	13.1 General introduction
	13.2 The chemical biology approach to gene therapy
	13.3 Biophysical characterisation of LNPs
	13.4 Applications of LNPs with small molecule drugs
14 Chemical Biology and Advanced Diagnostics Leading to Precision Therapeutic Approaches
	14.1 General introduction
	14.2 MRI basic principles leading to diagnostic applications
	14.3 PET/CT and SPECT fundamentals
	14.4 Understanding how to control nanoparticle biodistribution behaviour in vivo
	14.5 Theranostics
15 DNA Nanotechnology
	15.1 Background
	15.2 Three-dimensional DNA nanostructures
	15.3 Dynamic DNA nanostructures
	15.4 Biomedical applications of DNA nanostructures
Bibliography
Index
EULA