Enzymology at the Membrane Interface: Intramembrane Proteases

Content: 
Series PagePage ii
CopyrightPage iv
ContributorsPages xi-xiii
PrefacePages xv-xixMichael H. Gelb
Chapter One - Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P ProteasePages 1-33Y. Hizukuri, K. Akiyama, Y. Akiyama
Chapter Two - Signal Peptidase Enzymology and Substrate Specificity ProfilingPages 35-57R.E. Dalbey, D. Pei, Ö.D. Ekici
Chapter Three - Screening and Characterization Strategies for Nanobodies Targeting Membrane ProteinsPages 59-97S. Veugelen, M. Dewilde, B. De Strooper, L. Chávez-Gutiérrez
Chapter Four - Probing the Activity of Eukaryotic Rhomboid Proteases In VitroPages 99-126B. Cordier, M.K. Lemberg
Chapter Five - Expression, Purification, and Enzymatic Characterization of Intramembrane ProteasesPages 127-155R. Zhou, Y. Shi, G. Yang
Chapter Six - Analyzing Amyloid-β Peptide Modulation Profiles and Binding Sites of γ-Secretase ModulatorsPages 157-183J. Trambauer, A. Fukumori, B. Kretner, H. Steiner
Chapter Seven - Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility MethodPages 185-205T. Tomita
Chapter Eight - A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman SpectroscopyPages 207-228J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi, I. Ubarretxena-Belandia
Chapter Nine - An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the MembranePages 229-253R.P. Baker, S. Urban
Chapter Ten - Production of Recombinant Rhomboid ProteasesPages 255-278E. Arutyunova, R. Panigrahi, K. Strisovsky, M.J. Lemieux
Chapter Eleven - Mechanism and Inhibition of Rhomboid ProteasesPages 279-293K. Strisovsky
Chapter Twelve - Enzymatic Assays for Studying Intramembrane ProteolysisPages 295-308D.M. Bolduc, D.J. Selkoe, M.S. Wolfe
Chapter Thirteen - Methods for Structural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA SuperfamilyPages 309-347Y. Yang, N. Ke, S. Liu, W. Li
Chapter Fourteen - Functional Study of the Vitamin K Cycle Enzymes in Live CellsPages 349-394J.-K. Tie, D.W. Stafford
Chapter Fifteen - Activity Assays for Rhomboid ProteasesPages 395-437E. Arutyunova, K. Strisovsky, M.J. Lemieux
Author IndexPages 439-464
Subject IndexPages 465-474