Enzymes of Epigenetics, Part A

Content: 
Series Page Page ii  
Copyright Page iv  
Contributors Pages xiii-xvi  
Preface Pages xvii-xix Ronen Marmorstein 
Chapter One - In Vitro Chromatin Assembly: Strategies and Quality Control Pages 3-41 U. Muthurajan, F. Mattiroli, S. Bergeron, K. Zhou, Y. Gu, S. Chakravarthy, P. Dyer, T. Irving, K. Luger 
Chapter Two - Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes Pages 43-65 R.K. McGinty, R.D. Makde, S. Tan 
Chapter Three - Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins Pages 67-96 N. Sekulic, B.E. Black 
Chapter Four - Functional Characterization of Histone Chaperones Using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition Pages 97-117 C. Clément, I. Vassias, D. Ray-Gallet, G. Almouzni 
Chapter Five - Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes Pages 119-135 C.Y. Zhou, G.J. Narlikar 
Chapter Six - In Vitro Activity Assays for MYST Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening Pages 139-160 C.E. McCullough, R. Marmorstein 
Chapter Seven - Preparation and Biochemical Analysis of Classical Histone Deacetylases Pages 161-181 A. Villagra, E. Sahakian, E. Seto 
Chapter Eight - Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases Pages 183-208 B. Suenkel, C. Steegborn 
Chapter Nine - Preparation, Biochemical Analysis, and Structure Determination of SET Domain Histone Methyltransferases Pages 209-240 E. Bergamin, J.F. Couture 
Chapter Ten - Expression, Purification, and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase Pages 241-259 B. Laurent, Y. Shi 
Chapter Eleven - LSD1 Histone Demethylase Assays and Inhibition Pages 261-278 D. Hayward, P.A. Cole 
Chapter Twelve - Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases Pages 279-301 S. Krishnan, R.C. Trievel 
Chapter Thirteen - Preparation and Analysis of Native Chromatin-Modifying Complexes Pages 303-318 Y. Doyon, J. Côté 
Chapter Fourteen - Preparation, Biochemical Analysis, and Structure Determination of the Bromodomain, an Acetyl-Lysine Binding Domain Pages 321-343 C. Ren, L. Zeng, M.-M. Zhou 
Chapter Fifteen - Preparation, Biochemical Analysis, and Structure Determination of Methyllysine Readers Pages 345-362 C.A. Musselman, T.G. Kutateladze 
Chapter Sixteen - Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity Pages 365-385 M.Y. Liu, J.E. DeNizio, R.M. Kohli 
Chapter Seventeen - Characterization of How DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins Pages 387-401 A. Patel, H. Hashimoto, X. Zhang, X. Cheng 
Chapter Eighteen - Crystallographic Studies of Telomerase Pages 403-419 H. Hoffman, E. Skordalakes 
Chapter Nineteen - Detection and Analysis of Long Noncoding RNAs Pages 421-444 F. Lai, E. Blumenthal, R. Shiekhattar 
Chapter Twenty - Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo Pages 445-466 D. Quénet, D. Sturgill, Y. Dalal 
Author Index Pages 467-495  
Subject Index Pages 497-507