Enzyme Structure Part I

Content: 
Contributors to volume 91
Pages xi-xiv

Preface
Page xv
C.H.W. Hirs, S.N. Timasheff

Volumes in series
Pages xvii,xix-xxv

[1] A buffer system for amino acid analyzers with automatic integrators Original Research Article
Pages 3-8
C.H.W. Hirs

[2] Analysis for methylated amino acids in proteins Original Research Article
Pages 8-13
Marshall Elzinga, Nicholas Alonzo

[3] Analysis for Оі-carboxyglutamic acid Original Research Article
Pages 13-17
Paul A. Price

[4] Analysis for imino acids with o-phthalaldehyde Original Research Article
Pages 17-26
Peter Böhlen

[5] Single hydrolysis method for all amino acids, including cysteine and tryptophan Original Research Article
Pages 26-36
A.S. Inglis

[6] Amino acid composition of proteins eluted from polyacrylamide gels Original Research Article
Pages 36-41
William E. Brown, Gary C. Howard

[7] Amino acid analysis in the picomole range by precolumn derivatization and high-performance liquid chromatography Original Research Article
Pages 41-48
Jui-Yoa Chang, RenГ© Knecht, Dietmar G. Braun

[8] Reassessment of Ellman's reagent Original Research Article
Pages 49-60
Peter W. Riddles, Robert L. Blakeley, Burt Zerner

[9] Relating proteins by amino acid composition Original Research Article
Pages 60-75
Athel Cornish-Bowden

[10] Amino-terminal analysis with dimethylaminoazobenzene isothiocyanate Original Research Article
Pages 79-84
Jui-Yoa Chang

[11] Micro-identification of amino-terminal acetylamino acids in proteins by using high-performance liquid chromatography Original Research Article
Pages 84-92
Susumu Tsunasawa, Kozo Narita

[12] Determination of total protein Original Research Article
Pages 95-119
Gary L. Peterson

[13] Analysis of metal ions in proteins by X-ray fluorescence spectrometry Original Research Article
Pages 119-137
Serge N. Vinogradov, Michael J. Rokosz

[14] High-performance liquid chromatography of proteins Original Research Article
Pages 137-190
Fred E. Regnier

[15] Very-high-resolution two-dimensional electrophoretic separation of proteins on giant gels Original Research Article
Pages 190-214
Donald A. Young, Bruce P. Voris, Edward V. Maytin, Robert A. Colbert

[16] Preparative polyacrylamide gel electrophoresis Original Research Article
Pages 214-226
Steven Spiker, Irvin Isenberg

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis Original Research Article
Pages 227-236
Michael W. Hunkapiller, Eva Lujan, Frank Ostrander, Leroy E. Hood

[18] Staining of proteins on gels: Comparisons of dyes and procedures Original Research Article
Pages 236-247
Curtis M. Wilson

[19] Sensitive detection of proteins and peptides in polyacrylamide gels after formaldehyde fixation Original Research Article
Pages 247-254
Robert R. BГјrk, Margret Eschenbruch, Peter Leuthard, Germaine Steck

[20] Removal of sodium dodecyl sulfate from proteins by ion-pair extraction Original Research Article
Pages 254-259
William H. Konigsberg, Lou Henderson

[21] Removal of sodium dodecyl sulfate from proteins by ion-retardation chromatography Original Research Article
Pages 259-263
Serge N. Vinogradov, Oscar H. Kapp

[22] Recovery of functional proteins in sodium dodecyl sulfate gels Original Research Article
Pages 263-277
Ad Spanos, Ulrich HГјbscher

[23] Desalting protein solutions in a centrifuge column Original Research Article
Pages 278-281
Richard I. Christopherson

[24] Synthesis of carrier ampholytes for isoelectric focusing Original Research Article
Pages 281-298
Wilhelm W. Just

[25] Use of noncommercial carrier ampholytes for chromatofocusing Original Research Article
Pages 299-308
Wilhelm W. Just

[26] Cleavage at tryptophan with o-iodosobenzoic acid Original Research Article
Pages 311-318
Angelo Fontana, Daniele Dalzoppo, Claudio Grandi, Marcello Zambonin

[27] Cleavage at tryptophanyl residues with dimethyl sulfoxide-hydrochloric acid and cyanogen bromide Original Research Article
Pages 318-324
Henry V. Huang, Martha W. Bond, Michael W. Hunkapiller, Leroy E. Hood

[28] Cleavage at aspartic acid Original Research Article
Pages 324-332
A.S. Inglis

[29] Countercurrent chromatography Original Research Article
Pages 335-351
Yoichiro Ito

[30] Separation of peptides by reversed-phase high-performance liquid chromatography Original Research Article
Pages 352-359
Mark Hermodson, Walter C. Mahoney

[31] Analyzer for microscale peptide separations Original Research Article
Pages 359-367
Heinz Nika, Tore Hultin

[32] Purification of peptides that contain methionine residues Original Research Article
Pages 367-377
Jack Kyte, Jay Degen, Richard N. Harkins

[33] Immobilized anhydrotrypsin as a specific affinity adsorbent for tryptic peptides Original Research Article
Pages 378-383
Shin-ichi Ishii, Hideyoshi Yokosawa, Takashi Kumazaki, Izumi Nakamura

[34] High-resolution analytical and preparative peptide mapping by a combination of ion-exchange chromatography and thin-layer chromatography Original Research Article
Pages 384-391
Debra K. Aromatorio, Judy Parker, William E. Brown

[35] Isolation of sulfhydryl peptides alkylated with N-ethylmaleimide by diagonal electrophoresis Original Research Article
Pages 392-396
Heinz Gehring, Philipp Christen

[36] High-sensitivity sequencing with a gas-phase sequenator Original Research Article
Pages 399-413
Michael W. Hunkapiller, Rodney M. Hewick, William J. Dreyer, Leroy E. Hood

[37] Radiochemical sequence analysis of biosynthetically labeled proteins Original Research Article
Pages 413-434
John E. Coligan, Frederick T. Gates III, Edward S. Kimball, W. Lee Maloy

[38] Improvement of efficiency of commercial spinning-cup sequenators Original Research Article
Pages 434-442
Ajit S. Bhown, J. Claude Bennett

[39] Modified extraction procedure in spinning-cup sequenators Original Research Article
Pages 443-450
A.S. Inglis

[40] High-sensitivity sequence analysis of proteins recovered from sodium dodecyl sulfate gels Original Research Article
Pages 450-455
Ajit S. Bhown, J. Claude Bennett

[41] Manual micro-sequence analysis of polypeptides using dimethylaminoazobenzene isothiocyanate Original Research Article
Pages 455-466
Jui-Yoa Chang

[42] Thin-layer peptide mapping with sequencing at the nanomole level Original Research Article
Pages 466-486
Dennis A. Powers, James C. Fishbein, Allen R. Place

[43] Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography Original Research Article
Pages 486-493
Michael W. Hunkapiller, Leroy E. Hood

[44] High-performance liquid chromatography of side chain-protected phenylthiohydantoins: Application to solid-phase peptide synthesis Original Research Article
Pages 494-502
David H. Schlesinger

[45] Chemical ionization mass spectral analysis of phenylthiohydantoin derivatives Original Research Article
Pages 502-511
Thomas Fairwell

[46] Polypeptide sequencing with dipeptidyl peptidases Original Research Article
Pages 511-524
Henry C. Krutzsch

[47] Establishing homologies in protein sequences Original Research Article
Pages 524-545
Margaret O. Dayhoff, Winona C. Barker, Lois T. Hunt

[48] Reduction of sulfoxides in peptides and proteins Original Research Article
Pages 549-559
Richard A. Houghten, Choh Hao Li

[49] Amidination Original Research Article
Pages 559-569
John K. Inman, Richard N. Perham, Garrett C. Dubois, Ettore Appella

[50] Protein labeling by reductive alkylation Original Research Article
Pages 570-579
Neil Jentoft, Dorr G. Dearborn

[51] Bifunctional reagents Original Research Article
Pages 580-609
Tae H. Ji

[52] Photoaffinity labeling of nucleotide binding sites with 8-azidopurine analogs: Techniques and applications Original Research Article
Pages 613-633
Robert L. Potter, Boyd E. Haley

[53] Labeling Acyl-CoA binding sites with photolabile analogs Original Research Article
Pages 633-642
Roland E. Barden, Fidelis M. Achenjang, Christopher M. Adams

Author index
Pages 643-665

Subject index
Pages 667-693