Enzyme purification and related techniques

Content: 
Contributors to volume XXII
Pages ix-x

Preface
Pages xi-xii
William B. Jakoby

Volumes in series
Pages xiii-xv

[1] Buffer preparation Original Research Article
Pages 3-5
Robert J. Johnson, David E. Metzler

[2] Automated enzyme analysis-general Original Research Article
Pages 5-14
Morton K. Schwartz

[3] Automated multiple enzyme analysis systems Original Research Article
Pages 14-22
A.L. Tappel

[4] Dialysis Original Research Article
Pages 23-32
Peter McPhie

[5] Lyophilization Original Research Article
Pages 33-39
Johannes Everse, Francis E. Stolzenbach

[6] Ultrafiltration for enzyme concentration Original Research Article
Pages 39-49
William F. Blatt

[7] An anaerobic laboratory Original Research Article
Pages 49-54
J.Michael Poston, Thressa C. Stadtman, Earl R. Stadtman

[8] Enrichment culture and isolation techniques particularly for anaerobic bacteria Original Research Article
Pages 57-64
Jay V. Beck

[9] Culture of anaerobic bacteria for biochemical studies Original Research Article
Pages 64-70
Thressa C. Stadtman

[10] Molds and streptomycetes Original Research Article
Pages 70-80
D. Perlman

[11] Neurospora crassa: Preparative scale for biochemical studies Original Research Article
Pages 80-86
Carl Rhodes, John Germershausen, S.R. Suskind

[12] Increasing enzyme production by genetic and environmental manipulations Original Research Article
Pages 86-95
Arnold L. Demain

[13] Bacterial Membranes Original Research Article
Pages 99-120
H.R. Kaback

[14] Yeast Spheroplasts Original Research Article
Pages 120-122
Enrico Cabib

[15] Isolation of plasma membranes from mammalian cells Original Research Article
Pages 123-130
E.H. Eylar, Arpi Hagopian

[16] Isolation of Golgi apparatus Original Research Article
Pages 131-148
D.James MorrГ©

[17] Preparation and processing of small samples of human material Original Research Article
Pages 149-168
James A. Boyle, J.Edwin Seegmiller

[18] Zonal centrifugation Original Research Article
Pages 168-204
George B. Cline, Richard B. Ryel

[19] Extraction of water-soluble enzymes and proteins from membranes Original Research Article
Pages 204-219
Harvey S. Penefsky, Alexander Tzagoloff

[20] Extraction and purification of lipoprotein complexes from membranes Original Research Article
Pages 219-230
Alexander Tzagoloff, Harvey S. Penefsky

[21] Fractionation of protein mixtures with organic solvents Original Research Article
Pages 233-238
Seymour Kaufman

[22] Water-soluble nonionic polymers in protein purification Original Research Article
Pages 238-248
Melvin Fried, Paul W. Chun

[23] Crystallization as a purification technique Original Research Article
Pages 248-252
William B. Jakoby

[24] Formation of large crystals Original Research Article
Pages 253-266
Michael Zeppezauer

[25] Protein crystallization: Micro techniques involving vapor diffusion Original Research Article
Pages 266-269
David R. Davies, D.M. Segal

[26] Chromatography of proteins on ion-exchange adsorbents Original Research Article
Pages 273-286
S. Ralph Himmelhoch

[27] Gel filtration Original Research Article
Pages 287-321
John Reiland

[28] A method for anaerobic column chromatography Original Research Article
Pages 322-325
R. Repaske

[29] Chromatography of proteins on hydroxyapatite Original Research Article
Pages 325-339
Giorgio Bernardi

[30] Preparation of calcium phosphate gel deposited on cellulose Original Research Article
Pages 339-342
Masahiko Koike, Minoru Hamada

[31] Affinity chromatography Original Research Article
Pages 345-378
Pedro Cuatrecasas, Christian B. Anfinsen

[32] Specific elution with substrate Original Research Article
Pages 379-385
Burton M. Pogell, M.G. Sarngadharan

[33] Isoelectric focusing of proteins Original Research Article
Pages 389-412
Olof Vesterberg

[34] Preparative acrylamide gel electrophoresis: Continuous and disc techniques Original Research Article
Pages 412-433
Louis Shuster

[35] Preparative gel-density gradient electrophoresis Original Research Article
Pages 434-437
Louis Shuster

[36] Large-scale growth of bacteria Original Research Article
Pages 441-476
E.F. Phares

[37] Scale-Up of protein isolation Original Research Article
Pages 476-556
Stanley E. Charm, Charles C. Matteo

[38] Gel electrofocusing Original Research Article
Pages 559-564
C.W. Wrigley

[39] Analytical disc gel electrophoresis Original Research Article
Pages 565-578
Othmar Gabriel

[40] Locating enzymes on gels Original Research Article
Pages 578-604
Othmar Gabriel

Author index
Pages 605-622

Subject index
Pages 623-648