Enzyme Engineering: Volume 4

Front Matter....Pages i-xx
Front Matter....Pages 1-1
Genetic Engineering....Pages 3-6
The Photosynthetic Apparatus....Pages 7-16
Front Matter....Pages 17-17
Increasing the Production of Enzymes Via Fermentation....Pages 19-23
Cultural Conditions for the Microbial Production of β-Tyrosinase and Tryptophanase....Pages 25-31
Batch Culture Experiments in the Optimization of Continuous Fermentation of an Intracellular Enzyme....Pages 33-39
Properties of Heat Stable Enzymes of Extreme Thermophiles....Pages 41-46
Aqueous Two-Phase Systems for the Large-Scale Purification of Enzymes....Pages 47-53
Procedure for the Simultaneous Large-Scale Isolation of Pullulanase and 1,4-α-Glucan Phosphorylase Involving Liquid-Liquid Separations....Pages 55-56
Purification and Immobilization of a Fungal ß-Galactosidase (Lactase)....Pages 57-59
A Xylanase from Schizophyllum commune ....Pages 61-63
Front Matter....Pages 65-65
Immobilized Lactase for Whey Hydrolysis: Stability and Operating Strategy....Pages 67-76
Reaction Engineering Aspects of Continuous Operation with Biocatalysts....Pages 77-81
Economical Aspects of Continuous Operation with Biocatalysis....Pages 83-88
Pressure Drop Across Compressible Beds....Pages 89-92
Heterogeneous Kinetics of Two-Substrate Enzymic Reactions Under Diffusional Limitations....Pages 93-94
Membrane Reactor-Separator for Continuous Enzymatic Regeneration of ATP....Pages 95-98
Immobilized Glucose-Oxidase/Catalase: Deactivation in a Differential Reactor....Pages 99-100
Effect of Design Parameter on the Performance of Immobilized Glucose Isomerase Reactor System....Pages 101-103
Immobilization of Acetate Kinase on Functionalized Solid-Core Polymeric Beads....Pages 105-108
Front Matter....Pages 109-109
Surface Modification of Proteins....Pages 111-116
Front Matter....Pages 109-109
Staphylococcal Nuclease Immobilized on Agarose Gel....Pages 117-122
Enzyme Collagen Membranes in Reactors and for Analytical Purposes....Pages 123-127
Analysis of the Reaction Kinetics of Single Esterasesepharose Beads by Microfluorometry....Pages 129-132
A New Kinetic Method for Analysing the Parameters of Immobilized Aldolase....Pages 133-135
Interaction of Enzymes with Synthetic Polymers....Pages 137-139
Proteolysis of Immobilized Lactate Dehydrogenase in the Presence of Pyruvate and NADH....Pages 141-142
Synthesis and Kinetic Properties of a New NAD + Derivative Carrying a Vinyl Group....Pages 143-145
Isocyanide Derivatives of Polysaccharides as Supports for Enzyme Immobilization....Pages 147-149
Enzymes on Magnetic Supports....Pages 151-152
Oxirane-Acrylic Beads, Preparation 2878-C....Pages 153-154
Immobilization of 3β-Hydroxysteroid Dehydrogenase Isolated from Streptomyces griseocarneus ....Pages 155-157
A Method to Prepare Bead-Shaped Immobilized Enzyme....Pages 159-160
Immobilization of Hydrogenase from Alcaligenes Eutrophus H 16 on Glass Beads....Pages 161-163
Hemisynthesis of Aminoglycosides....Pages 165-166
Front Matter....Pages 167-167
Enzyme-Polyethylene Glycol Adducts: Modified Enzymes with Unique Properties....Pages 169-173
Carrier-Bound Detoxifying Enzymes for an Extracorporeal Detoxification of Endogenous and Exogenous Toxins....Pages 175-177
Biomedical Applications of Enzymatic Fibres....Pages 179-186
Liposomes as Carriers of Proteins: Possible Medical Applications....Pages 187-192
Galactose Conversion Using a Microcapsule Immobilized Multienzyme Cofactor Recycling System....Pages 193-197
Hollow Fiber-Entrapped Liver Microsomes: A Potential Extracorporeal Drug Detoxifier....Pages 199-206
Front Matter....Pages 167-167
Enzymatic Conversion of Cyclohexane to Cyclohexanol by Isolated Rat Liver Microsomes (MS)....Pages 207-208
Hydrolysis of Glucuronides with Immobilized Enzymes....Pages 209-210
Immobilization of Neuraminidase for the Treatment of Tumor Cells....Pages 211-212
Enzyme Thermistor Analysis in Clinical Chemistry and Environmental and Process Control....Pages 213-216
Nylon Tube Bound Enzymes in Systems for the Determination of Substrates in Serum and Urine by Electrochemical and Calorimetric Detectors....Pages 217-218
Analytical Application of Collagen Enzymatic Membranes for a Glucose Sensor....Pages 219-221
Immobilization of Enzyme on PMG and Its Application to the Urea Monitoring Apparatus....Pages 223-225
Front Matter....Pages 227-227
Industrial Applications of Immobilized Enzymes: State of the ART....Pages 229-236
Regeneration of ATP by Immobilized Microbial Cells and Its Utilization for the Synthesis of Nucleotides....Pages 237-244
Production of 6APA in the Penicillin G Fermentation Plant by Using Fiber-Entrapped Penicillin Amidase....Pages 245-252
Enzymatic Deacetylation of Cephalosporins....Pages 253-259
Enzymatic Production of Sugars from Hemicellulose....Pages 261-266
Enzymes and Separation Processes....Pages 267-271
Enzymatic Hydrolysis and Protein Ultrafiltration for Converting Acid Whey to Valuable Products....Pages 273-273
Model Proteolysis of β-Casein with Immobilized Trypsin and Its Technological Significance....Pages 275-276
Detoxification of Organophosphate Pesticides Using Immobilized Enzymes....Pages 277-278
Production of L-Tryptophan....Pages 279-280
Electrochemical Assay of Oxidoreductase Enzymes....Pages 281-282
Design Considerations and Operational Strategies of a Commercial Glucose Isomerase Reactor System....Pages 283-286
Front Matter....Pages 287-287
Enzyme Sequences in the Living Cell....Pages 289-297
Front Matter....Pages 287-287
Immobilization of Enzymes, Microbial Cells, and Organelles by Inclusion with Photo-Crosslinkable Resins....Pages 299-306
Immobilized Cell Systems....Pages 307-316
Steroid Conversion Using Immobilized Living Microorganisms....Pages 317-322
Microbial Cells Immobilized and Living on Solid Supports and their Application to Fermentation Processes....Pages 323-328
Microbial Electrode: BOD Sensor....Pages 329-333
New Method for Immobilization of Microbial Cells and its Industrial Application....Pages 335-337
Polymer Entrapment of Microbial Cells: Preparation and Reactivity of Catalytic Systems....Pages 339-341
Benzene Metabolism by Bacterial Cells Immobilized in Polyacrylamide Gel....Pages 343-344
Cellulose Bead Entrapped Whole Cell Glucose Isomerase in Fructose Syrup Production....Pages 345-347
Properties of a Cell-Bound Glucose Isomerase....Pages 349-351
Front Matter....Pages 353-353
Hydrophobic Interactions in Purification and Utilization of Enzymes....Pages 355-363
Relative Importance of Diffusion Layer Resistance and Microenvironmental Effects on the Effectiveness of Immobilized Enzyme Reactors....Pages 365-371
Studies of Hemoglobin and Allosteric Enzymes Under Artificial Conformational Constraints....Pages 373-379
Determination of Michaelis Menten Parameters for Immobilized Enzymes: Detection and Measurement of Diffusion....Pages 381-384
Studies with Immobilized Toxins....Pages 385-389
Immobilized Protein Modification Reagents....Pages 391-392
On the Mechanism of Protein Cross-Linking with Glutaral-Dehyde....Pages 393-394
Synthesis of Water Soluble Polymers with Covalently Bound General Ligands....Pages 395-397
Soluble Nad + -Derivatives of High Molecular Weight in Enzymic Recycling Systems....Pages 399-400
The Use of Enzymes for Design of Light Sensitive Silver-Less Materials: Photoenzography....Pages 401-402
Front Matter....Pages 353-353
Continuous Measurements on Immobilized Cells by a Mass Filter....Pages 403-404
Enzyme Activity at a Gas Solid Interphase: Oxidation of Methanol to Formaldehyde....Pages 405-407
Some Reactive Carriers and Immobilized Enzymes....Pages 409-412
Immobilization of Proteins on Polyhydrazides....Pages 413-414
Studies of Insoluble Organic/Inorganic Composites of Glucose Isomerase....Pages 415-416
Front Matter....Pages 417-417
Pressure-Driven Affinity Sorption of Trypsin Inhibitors....Pages 419-425
Measurement of ATP And Ligand-ATP Conjugates by Enzymic Cycling with Co-Immobilized Hexokinase and Pyruvate Kinase....Pages 427-434
Affinity Therapy....Pages 435-440
Principles of Multi-Enzyme Purification by Affinity Chromatography....Pages 441-442
Affinity Chromatography of Proteolytic Enzymes....Pages 443-444
Guanosine Nucleotide Analogues as General Ligands in Affinity Chromatography....Pages 445-447
Physical-Chemical Factors Influencing the Resolution in Affinity Chromatography....Pages 449-450
Use of Affinity Chromatography for Determination of Dissociation Constants of Complexes of Trypsin and Chymotrypsin with their Free and Immobilized Inhibitors....Pages 451-452
Purification of Pyruvate Dehydrogenase by Affinity Chromatography....Pages 453-455
Studies on the Purification of Aspergillus niger Endopolygalacturonase by Agarose Gel Chromatography....Pages 457-458
Affinity Chromatography: Recent Advances....Pages 459-460
Back Matter....Pages 461-492