Dynamin Superfamily GTPases: Methods and Protocols

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
 Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae  (Nolan W. Kennedy, Lora K. Picton, R. Blake Hill)....Pages 3-15
 Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems  (Natalia V. Varlakhanova, Marijn G. J. Ford)....Pages 17-27
Front Matter ....Pages 29-29
 A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli  (Natalia Stepanyants, Patrick J. Macdonald, Pooja Madan Mohan, Rajesh Ramachandran)....Pages 31-40
 Affinity Purification and Functional Characterization of Dynamin-Related Protein 1  (Ryan W. Clinton, Brianna L. Bauer, Jason A. Mears)....Pages 41-53
 Purification and Characterization of MxB  (Frances Joan D. Alvarez, Peijun Zhang)....Pages 55-65
 Purification of FarnesylatedhGBP1 and Characterization of Its Polymerization and Membrane Binding  (Linda Sistemich, Christian Herrmann)....Pages 67-81
Front Matter ....Pages 83-83
 Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)–Lipid Interactions  (Nikhil Bharambe, Rajesh Ramachandran)....Pages 85-92
 Nucleotide-Dependent Dimerization and Conformational Switching of Atlastin  (John P. O’Donnell, Carolyn M. Kelly, Holger Sondermann)....Pages 93-113
 Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System  (Tadato Ban, Naotada Ishihara)....Pages 115-127
 Cell-Free Analysis of Mitochondrial Fusion by Fluorescence Microscopy  (Nyssa Becker Samanas, Suzanne Hoppins)....Pages 129-140
 Electrophysiological Methods for Detection of Membrane Leakage and Hemifission by Dynamin 1  (Pavel V. Bashkirov, Ksenia V. Chekashkina, Anna V. Shnyrova, Vadim A. Frolov)....Pages 141-162
 Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore  (Katherine A. Smith, Emily R. Prantzalos, Arun Anantharam)....Pages 163-178
 Cellular Assays for Measuring Dynamin Activity in Muscle Cells  (Jessica Laiman, Ya-Wen Liu)....Pages 179-187
 Measuring Drp1 Activity in Mitochondrial Fission In Vivo  (Di Hu, Xin Qi)....Pages 189-195
 Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells  (Di Hu, Xin Qi)....Pages 197-204
 Imaging Dynamin-Related Protein 1 (Drp1)-Mediated Mitochondrial Fission in Living Cells  (Felipe Montecinos-Franjola, Rajesh Ramachandran)....Pages 205-217
 Correction to: Imaging Dynamin-Related Protein 1 (Drp1)-Mediated Mitochondrial Fission in Living Cells  (Felipe Montecinos-Franjola, Rajesh Ramachandran)....Pages C1-C1
Back Matter ....Pages 219-221