Dynamics of Membrane Proteins and Cellular Energetics

Front Matter....Pages I-XIII
Front Matter....Pages 1-1
The Structure and Function of Ca +2 - and Phospholipid- Dependent Protein Kinase (Protein Kinase C), A Trans-Membrane Signal Transducer....Pages 2-18
Biosynthesis and Mode of Insertion of a Stalked Intrinsic Membrane Protein of the Small Intestinal Brush Border....Pages 19-40
Tools and Approaches to Study Membranes and Cell Organelles Biogenesis....Pages 41-68
Fluid-Phase Endocytosis and Lysosomal Enzyme Excretion Two Facets of Intracellular Membrane Traffic....Pages 69-82
The Neutrophil Leukocyte. Properties and Mechanism of Activation....Pages 83-97
Monoclonal Antibodies for Immunoanalysis....Pages 98-105
Front Matter....Pages 107-107
Trans-Membrane Signaling Via Protein Kinase C and Its Inhibition by Staurosporine....Pages 108-122
Endocytosis Analysis by Flow Cytofluorometry : Study of the Endocytosis of Fluoresceinylated Neoglycoproteins Via Membrane Lectins of Tumor Cells....Pages 123-133
Neutrophils. Respiratory Burst and Exocytosis....Pages 134-153
Endocytosis and Exocytosis in Dictyostelium Amoebae....Pages 154-167
Isolation of Intact Chloroplasts - Criteria of Integrity....Pages 168-179
Methods for the Isolation of Higher Plant Vacuoles....Pages 180-203
Assay of in Vitro Microtubule Assembly. Effect of Microtubule Associated Proteins....Pages 204-210
Histamine Release from Human Basophiles....Pages 211-221
Intracellular Ca 2+ Measurements in Intact Neutrophils....Pages 222-230
Synthesis and Maturation of D-β-Hydroxybutyrate Dehydrogenase (BDH) from Mitochondrial Inner Membrane....Pages 231-250
Electropermeabilization and Electrofusion of Cells....Pages 251-268
Use of Monoclonal Antibody Against Keratin in Immunocytochemical Techniques....Pages 269-278