Digital Microscopy

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xv-xviii

Preface
Pages xix-xx
Greenfield Sluder, David E. Wolf

Chapter 1 - Microscope Basics
Pages 1-10
Greenfield Sluder, Joshua J. Nordberg

Chapter 2 - The Optics of Microscope Image Formation
Pages 11-42
David E. Wolf

Chapter 3 - Proper Alignment of the Microscope
Pages 43-67
Rudi Rottenfusser

Chapter 4 - Fundamentals of Fluorescence and Fluorescence Microscopy
Pages 69-97
David E. Wolf

Chapter 5 - Fluorescent Protein Applications in Microscopy
Pages 99-123
Whitney L. Johnson, Aaron F. Straight

Chapter 6 - Live-Cell Fluorescence Imaging
Pages 125-150
Jennifer C. Waters

Chapter 7 - Practical Aspects of Adjusting Digital Cameras
Pages 151-162
Joshua J. Nordberg, Greenfield Sluder

Chapter 8 - Cameras for Digital Microscopy
Pages 163-178
Kenneth R. Spring

Chapter 9 - A High-Resolution Multimode Digital Microscope System
Pages 179-210
Edward D. Salmon, Sidney L. Shaw, Jennifer C. Waters, Clare M. Waterman-Storer, Paul S. Maddox, Elaine Yeh, Kerry Bloom

Chapter 10 - Electronic Cameras for Low‐Light Microscopy
Pages 211-241
Ivan Rasnik, Todd French, Ken Jacobson, Keith Berland

Chapter 11 - Camera Technologies for Low Light Imaging: Overview and Relative Advantages
Pages 243-283
Butch Moomaw

Chapter 12 - Post-Processing for Statistical Image Analysis in Light Microscopy
Pages 285-315
Richard A. Cardullo, Edward H. Hinchcliffe

Chapter 13 - 65,000 Shades of Grey: Use of Digital Image Files in Light Microscopy
Pages 317-336
Sidney L. Shaw, Edward H. Hinchcliffe

Chapter 14 - Quantitative Analysis of Digital Microscope Images
Pages 337-367
David E. Wolf, Champika Samarasekera, Jason R. Swedlow

Chapter 15 - Evaluating Optical Aberrations Using Fluorescent Microspheres: Methods, Analysis, and Corrective Actions
Pages 369-385
Paul C. Goodwin

Chapter 16 - Ratio Imaging: Practical Considerations for Measuring Intracellular Ca2+ and pH in Living Cells
Pages 387-406
Nathan O\'Connor, Randi B. Silver

Chapter 17 - Quantitative Fluorescence Microscopy and Image Deconvolution
Pages 407-426
Jason R. Swedlow

Chapter 18 - Practical Aspects of Quantitative Confocal Microscopy
Pages 427-440
John M. Murray

Chapter 19 - Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy
Pages 441-456
Richard A. Cardullo

Chapter 20 - Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging
Pages 457-488
Leng-Chun Chen, William R. Lloyd III, Ching-Wei Chang, Dhruv Sud, Mary-Ann Mycek

Chapter 21 - Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells
Pages 489-524
Dylan A. Bulseco, David E. Wolf

Chapter 22 - Breaking the Resolution Limit in Light Microscopy
Pages 525-544
Rainer Heintzmann, Gabriella Ficz

Chapter 23 - Circumventing Photodamage in Live-Cell Microscopy
Pages 545-560
Valentin Magidson, Alexey Khodjakov

Chapter 24 - A User’s Guide to Localization-Based Super-Resolution Fluorescence Imaging
Pages 561-592
Graham T. Dempsey

Chapter 25 - Quantitative Ratiometric Imaging of FRET-Biosensors in Living Cells
Pages 593-609
Désirée Spiering, Jose Javier Bravo-Cordero, Yasmin Moshfegh, Veronika Miskolci, Louis Hodgson

Chapter 26 - Tip-Enhanced Raman Spectroscopy for the Base Interrogation of DNA
Pages 611-628
Noah Kolodziejski

Index
Pages 629-644

Volumes in Series
Pages 645-655