Developmental Toxicology: Methods and Protocols

Front Matter ....Pages i-xi
 In Vitro Models in Developmental Toxicology  (Louise M. Winn)....Pages 1-6
 Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments  (Jason M. Hansen, Ted B. Piorczynski)....Pages 7-17
 Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology  (Charles A. Easley)....Pages 19-34
 Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model  (Mika Suga, Miho K. Furue)....Pages 35-48
 Micromass Methods for the Evaluation of Developmental Toxicants  (Omar J. Mohammed, Margaret K. Pratten)....Pages 49-72
 The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System  (France-Hélène Paradis, Han Yan, Chunwei Huang, Barbara F. Hales)....Pages 73-91
 Embryonic Midfacial Palatal Organ Culture Methods in Developmental Toxicology  (Barbara D. Abbott)....Pages 93-105
 Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology  (Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, Sakhila K. Banu)....Pages 107-127
 Hepatotoxicity in Zebrafish Larvae  (Kathryn Bambino, Joshua Morrison, Jaime Chu)....Pages 129-138
 Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model  (Joslynn G. Affleck, Virginia K. Walker)....Pages 139-153
 The Chicken Embryo as a Model in Developmental Toxicology  (Michael R. Stark, Micah M. Ross)....Pages 155-171
 The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity  (Cecilia Berg)....Pages 173-186
 Mouse Whole Embryo Culture  (Emily W. Y. Tung, Louise M. Winn)....Pages 187-194
 Rat Whole Embryo Culture  (Craig Harris)....Pages 195-217
 Rabbit Whole Embryo Culture  (Terence R. S. Ozolinš)....Pages 219-233
 Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology  (Brendon K. Warner, Jonathan K. Alder, Arminda Suli)....Pages 235-250
 Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish  (Jonathon T. Hill)....Pages 251-259
 Assessment of Histiotrophic Nutrition Using Fluorescent Probes  (Craig Harris)....Pages 261-279
 Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization  (Jeffery R. Barrow)....Pages 281-295
 Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos  (Robert M. Zucker, John M. Rogers)....Pages 297-311
 Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection  (Danielle M. Drake, Aaron M. Shapiro, Peter G. Wells)....Pages 313-328
 Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA  (Shama Bhatia, Peter G. Wells)....Pages 329-349
 Western Analysis of Breast Cancer 1 Protein (BRCA1)  (Danielle M. Drake, Peter G. Wells)....Pages 351-374
 Regulation and Control of AP-1 Binding Activity in Embryotoxicity  (Terence R. S. Ozolinš)....Pages 375-388
 Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR)  (Shama Bhatia, Jason Matthews, Peter G. Wells)....Pages 389-403
 The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity  (Rebecca D. Maciver, Terence R. S. Ozolinš)....Pages 405-420
 Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining  (Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, Michelle Graf)....Pages 421-434
Back Matter ....Pages 435-436