Cytochromes P-450 and b5 : Structure, Function, and Interaction

Front Matter....Pages i-xiii
Partial Purification and Separation of Multiple Forms of Cytochrome P-450 and Cytochrome P-448 from Rat Liver Microsomes....Pages 1-24
Biochemical Characterization of Highly Purified Cytochrome P-450 and Other Components of the Mixed Function Oxidase System of Liver Microsomal Membranes....Pages 25-46
Immunochemical and Compositional Comparison of Cytochrome P-450 cam of Pseudomonas Putida and P-450 LM of Phenobarbital-Induced Rabbit Liver Microsomes....Pages 47-53
Immunochemical and Functional Similarities and Differences among Iron-Sulfur Proteins Involved in Mammalian Steroidogenesis....Pages 55-71
General Discussion....Pages 73-80
Comparison of the Induction Course, Biophysical Chemical Interactions and Photochemical Action Spectra of Phenobarbital and 3-Methylcholanthrene Induced Hepatic Microsomal P-450....Pages 81-102
Cytochrome P-450 in the Activation and Inactivation of Carcinogens....Pages 103-115
Effect of Cyclic Amp on the Phenobarbital Induced Increase in Cytochrome P-450 and Hypertrophy of the Endoplasmic Reticulum of the Rat Liver....Pages 117-126
Evidence for the Activation of 3-Methylcholanthrene as a Carcinogen in vivo and as a Mutagen in vitro by P 1 -450 from Inbred Strains of Mice....Pages 127-149
Increased Translation as a Result of Elevated Initiation Factor Activity after Administration of 3-Methylcholanthrene....Pages 151-164
Discussion....Pages 165-173
Optical and Electron Paramagnetic Resonance Studies of Partially Purified Rabbit Liver Cytochrome P-450....Pages 175-187
Studies on the Spin State of 3-Methylcholanthrene Induced Cytochrome P-450 from Rat Liver....Pages 189-202
An Analysis of the Optical Titrations of the 430 and 455 NM Chromophores of Ethyl Isocyanide Complexes of Mammalian Hepatic Cytochrome P-450....Pages 203-212
Implication of Ligand Modified Spectra of Cytochrome P-450 Associated with Pregnenolone Synthesis in Mitochondria from Corpus Luteum....Pages 213-227
General Discussion....Pages 229-237
Studies on the Interaction of Water with Microsomal Cytochrome P-450....Pages 239-249
Drug Metabolism in Isolated Rat Liver Cells....Pages 251-269
Role of Phospholipids in Adrenocortical Microsomal Hydroxylation Reactions: Activation of Lipid-Depleted Microsomal Preparations by Non-Ionic Detergents....Pages 271-286
On the Structure of Putidaredoxin and Cytochrome P-450 cam and their Mode of Interaction....Pages 287-309
Metabolic Control of Cytochrome P-450 cam ....Pages 311-324
General Discussion....Pages 325-333
Relationship Between Microsomal Hydroxylase and Glucuronyltransferase....Pages 335-341
A Possible Role of Copper in the Regulation of Heme Biosynthesis Through Ferrochelatase....Pages 343-354
Mixed Function Oxidation and Intermediary Metabolism: Metabolic Interdependencies in the Liver....Pages 355-367
Dissociation of Microsomal Ethanol Oxidation from Cytochrome P-450 Catalyzed Drug Metabolism....Pages 369-382
General Discussion....Pages 383-386
Interaction Between Microsomal Electron Transfer Pathways....Pages 387-404
Role of Cytochrome b 5 in the NADH Synergism of NADPH-Dependent Reactions of the Cytochrome P-450 Monooxygenase System of Hepatic Microsomes....Pages 405-434
The Role of Cytochrome b 5 in Cytochrome P-450 Enzymes....Pages 435-445
Role of Cytochrome b 5 in NADPH- and NADH-Dependent Hydroxylation by the Reconstituted Cytochrome P-450- or P-448-Containing System....Pages 447-466
The Role of Cytochrome b 5 in Mixed Function Oxidations: Effect of Microsomal Binding of the Hemoprotein on Hepatic N-Demethylations....Pages 467-483
Comparison of Methods to Study Enzyme Induction in Man....Pages 485-502
General Discussion....Pages 503-510
Back Matter....Pages 511-554