Corneal Regeneration: Methods and Protocols

Front Matter ....Pages i-xii
 Isolation and Culture of Corneal Stromal Stem Cells  (Richard M. Nagymihaly, Morten C. Moe, Goran Petrovski)....Pages 1-15
 In Vitro Expansion of Corneal Endothelial Cells for Transplantation  (Kim Santerre, Isabelle Xu, Mathieu Thériault, Stéphanie Proulx)....Pages 17-27
 Primary Culture of Cornea-Limbal Epithelial Cells In Vitro  (Finbarr O’Sullivan)....Pages 29-37
 Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant  (Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.)....Pages 39-49
 A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture  (James W. Foster, Karl J. Wahlin, Shukti Chakravarti)....Pages 51-58
 Gene Editing for Corneal Stromal Regeneration  (Tara Moore, Connie Chao-Shern, Larry DeDionisio, Kathleen A. Christie, M. Andrew Nesbit)....Pages 59-75
 Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery  (Liujiang Song, Jacquelyn J. Bower, Matthew L. Hirsch)....Pages 77-102
 The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea  (Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guérin)....Pages 103-118
 Formation of Corneal Stromal-Like Assemblies Using Human Corneal Fibroblasts and Macromolecular Crowding  (Mehmet Gürdal, Gülinnaz Ercan, Dimitrios I. Zeugolis)....Pages 119-141
 Preparation of Dried Amniotic Membrane for Corneal Repair  (Andrew Hopkinson, Emily R. Britchford, Laura E. Sidney)....Pages 143-157
 Fabrication of Corneal Extracellular Matrix-Derived Hydrogels  (Mark Ahearne, Julia Fernández-Pérez)....Pages 159-168
 Synthesis and Application of Collagens for Assembling a Corneal Implant  (Elle Edin, Fiona Simpson, May Griffith)....Pages 169-183
 Development and Validation of a 3D In Vitro Model to Study the Chemotactic Behavior of Corneal Stromal Fibroblasts  (Evrim Ceren Kabak, Julia Fernández-Pérez, Mark Ahearne)....Pages 185-196
 Femtosecond Laser-Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration  (Neil Lagali, Mehrdad Rafat)....Pages 197-214
 The Use of Animal Models to Assess Engineered Corneal Tissue  (Robert Thomas Brady, Peter W. Madden)....Pages 215-230
 X-Ray Diffraction Imaging of Corneal Ultrastructure  (Keith M. Meek, Andrew J. Quantock, Sally Hayes, James Bell)....Pages 231-247
Back Matter ....Pages 249-250