Comparative Genetic Toxicology: The Second UKEMS Collaborative Study

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Design of the Study ‘Scientific Rationale and Chemical Selection’....Pages 3-8
The Genotoxicity of Benzidine and 4-Dimethylaminoazobenzene: A Survey of the Literature....Pages 9-37
Front Matter....Pages 39-39
Evaluation of the Mutagenic Activity of 4-Dimethylaminoazobenzene (DAB) and 4-Cyanodimethylaniline (CDA) in the Salmonella/Microsome Assay....Pages 41-42
Salmonella Mutagenicity Testing of Benzidine, 4,4″-Diaminoterphenyl, 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline Using three Different S9 Preparations....Pages 43-47
The Mutagenic Activity of the Structurally Related Compounds Benzidine (BZD) and 4,4″-Diaminoterphenyl (DAT) in the Salmonella/Mammalian-Microsome Assay....Pages 49-53
Bacterial Mutagenicity of BZD and DAT....Pages 55-58
4-Dimethylaminoazobenzene (DAB) and 4-Cyanodimethylaniline (CDA): Reverse Mutation Tests with Salmonella typhimurium TA1538....Pages 59-61
Comparative Mutagenic Activity on Salmonella typhimurium TA1538 of Benzidine and its Terphenyl Analogue 4,4″-Diaminoterphenyl Using Chicken Liver, Aroclor-induced and Non-induced Rat Liver S9s....Pages 63-67
A Comparison of the Mutagenicity of Benzidine and 4,4″-Diaminoterphenyl with Uninduced Mouse and Rat Liver S9....Pages 69-71
The Testing of four UKEMS Trial Compounds for Bacterial Mutation in the Salmonella/Microsome Assay Using (1) Aroclor-induced Rat Liver, (2) Chicken Liver S9 and (3) The York Modification of the Standard Ames Protocol....Pages 73-77
Bacterial Mutation Tests on CDA and DAB Using Salmonella typhimurium TA1538 and three Types of S9 Activation....Pages 79-83
A Comparison of the Effects of Uninduced Mouse, Uninduced Rat and Aroclor-induced Rat Liver S9 on Reversion in Salmonella by DAB and CDA....Pages 85-88
Studies on the Metabolic Activation of Benzidine and its Terphenyl Derivative to Mutagens in the Salmonella Plate Incorporation Assay by Various Liver Preparations....Pages 89-92
Mutagenicity of BZD, DAT, DAB and CDA in the Bacterial Mutagenicity Test....Pages 93-98
Bacterial Mutagenicity Assays: Co-ordinators’ Report....Pages 99-102
Front Matter....Pages 103-144
Studies on the Mutagenicity of Benzidine and its Terphenyl Analogue in the Plate-incorporation Test Using Liver Microsome and Cytosol Fractions....Pages 145-145
The in vitro Mutagenicity of Benzidine and 4,4″-Diaminoterphenyl....Pages 147-153
Urinary Mutagenicity in Rats after Administration of Benzidine and its Terphenyl Analogue, 4,4″-Diamenoterphenyl....Pages 155-163
Enhanced Mutagenicity of DAT to Salmonella (-S9) when Evaluated under Conditions of Reduced Light....Pages 165-174
Front Matter....Pages 175-178
Weak Mutagenicity of 4,4″-Dinitroterphenyl to Salmonella in the Absence of S9 Mix....Pages 145-145
The Comparative Mutagenicity of 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline in Plate-incorporation Tests and Fluctuation Assays....Pages 179-180
Front Matter....Pages 181-187
The Comparative Sensitivity of Repair Proficient and Repair Deficient Strains of Yeast to BZD, DAT, DAB and CDA....Pages 189-192
The Induction of Mitotic Gene Conversion in Saccharomyces cerevisiae by the Compounds BZD, DAT, DAB and CDA....Pages 193-193
The Genotoxicity of BZD and DAT in Yeast Strain D7....Pages 195-203
Gene Mutation in Yeast Induced by DAB, CDA, BZD and DAT....Pages 205-209
The Induction of Mitotic Aneuploidy and Crossing-over in Yeast....Pages 211-220
Assay for Forward Mutation in Schizosaccharomyces pombe P1....Pages 221-229
A Summary of the Effects of Benzidine, 4,4″-Diaminoterphenyl, 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline as Observed by the Yeast Group in the Second UKEMS Collaborative Study....Pages 231-239
Front Matter....Pages 241-252
Mutagenicity of Benzidine and 4,4″-Diamenoterphenyl in Chinese Hamster V79 Cells....Pages 253-258
DNA Damage in the Rat Liver in vivo and Mutagenicity to V79 Cells in vitro for BZD, DAT, DAB and CDA....Pages 259-265
The Absence of Mutagenic Activity of Benzidine and 4,4″-Diaminoterphenyl in Tk6 Human Lymphoblast Cells....Pages 267-277
The Mutagenic Effects of DAB and CDA in a Thymidine Kinase-deficient Clone of Friend Mouse Leukaemia Cells....Pages 279-279
The Mutagenicity of Benzidine, 4,4″-Diaminoterphenyl, N,N-Dimethyl-4-aminoazobenzene and 4-Cyano-N,N-dimethylaniline in the L51787 Mouse Lymphoma Assay....Pages 281-289
The Induction of Trifluorothymidine Resistance in L5178Y Tk+/− Mouse Lymphoma Cells by 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline....Pages 291-299
Mammalian Cell Mutation Assays with Benzidine, 4,4″-Diaminoterphenyl, 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline....Pages 301-306
Front Matter....Pages 307-310
SCE Tests of Butter Yellow, 4-Cyanodimethylaniline, Benzidine and Diaminoterphenyl Using Chinese Hamster V79 Cells in the Presence of S9 Mix....Pages 311-318
A Comparison of the Chromosome Damaging Effects, in Cultured Human Lymphocytes, of Benzidine (BZD) and 4,4″-Diaminoterphenyl (DAT) in the Presence of Aroclor-induced and Uninduced Rat Liver S9....Pages 319-326
Induction of Chromosome Aberrations in Chinese Hamster Liver Cells Treated with BZD, DAT, DAB and CDA....Pages 327-332
Chromosome Analysis of Cultured Rat Liver Epithelial Cells Treated with BZD, DAT, DAB or CDA....Pages 333-338
The Effects of BZD, DAT, DAB and CDA on Mitotic Chromosome Aneuploidy and the Nuclear Spindle....Pages 339-339
Front Matter....Pages 341-354
Activity of Four UKEMS Trial Compounds in a HeLa Cell DNA Repair Assay....Pages 355-362
Activity of CDA, DAB and 6BT in the in vivo Rat Hepatocyte DNA Repair Assay....Pages 363-365
Front Matter....Pages 367-371
Preparation and Characterisation of S9 Fractions....Pages 373-380
Front Matter....Pages 381-385
Chromosome Studies in Hepatic Cells from Rats Exposed to Genotoxic Chemicals....Pages 387-396
4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline : Micronucleus Test in Alderley Park Wistar Derived Rats....Pages 397-397
Evaluation of Benzidine and 4,4″-Diaminoterphenyl in the Micronucleus Test....Pages 399-404
BZD and DAT: Investigation in two Mouse Bone Marrow Assays, Micronucleus Test and Chromosomal Aberration Test at Mitosis....Pages 405-410
4-Dimethylaminoazobenzene (DAB) and 4-Cyanodimethylaniline (CDA): An in vivo Cytogenetic Study in the Alderley Park (Wistar) Rat....Pages 411-411
Evaluation of the in vivo Cytogenetic Assays....Pages 413-438
Front Matter....Pages 439-439
Sex-linked Recessive Lethal Tests in Drosophila with Benzidine, 4,4″-Diaminoterphenyl, 4-Dimethylaminoazobenzene and 4′-Cyanodimethylaniline....Pages 441-451
Effects of 4-Dimethylaminoazobenzene (DAB) and 4-Cyanodimethylaniline (CDA) in the Mammalian Spot Test....Pages 453-459
A Comparison of the Tissue Distribution and Covalent Binding to Hepatic DNA of [3H]Benzidine and [3H]4,4″-Diaminoterphenyl in the Rat....Pages 461-465
Front Matter....Pages 467-473
Disposition of [14C]Cyanodimethylaniline in the Bone Marrow of the Rat....Pages 475-481
The Binding of 4-Cyano-N,N-dimethyl-[14C] aniline (CDA) Metabolites in the Rat....Pages 483-489
The Binding of 4-Cyano-N,N-dimethyl-[14C] aniline (CDA) Metabolites to Macromolecules in Mice Exposed to CDA....Pages 491-491
The Metabolism of 4-Cyano-N,N dimethyl-[14C] aniline (CDA) by Rats and Mice....Pages 493-497
The Carcinogenicity of Benzidine and its Analogue 4,4″-Diaminoterphenyl in the F344 Rat....Pages 499-505
The Effects of BZD and DAT on Nuclear Ploidy in the Rat Liver....Pages 507-513
Co-ordinator’s Report: In vivo Assays Used in the Second UKEMS Collaborative Study....Pages 515-525
Front Matter....Pages 491-491
An Overview of the Results of the in vivo and in vitro Test Systems Used to Assay the Genotoxicity of BZD, DAT, DAB and CDA in the Second UKEMS Collaborative Study....Pages 527-531
Back Matter....Pages 533-536
....Pages 537-542