Chromatin Structure and Function: Molecular and Cellular Biophysical Methods

Front Matter....Pages i-xix
Front Matter....Pages 1-1
Properties and Composition of Isolated Chromatin....Pages 3-13
Expressed and Nonexpressed Portions of the Genome: Their Separation and their Characterization....Pages 15-23
Front Matter....Pages 29-29
Electron Microscopy: A Tool for Visualizing Chromatin....Pages 31-40
Transcriptional Control of Native Chromatin....Pages 41-65
Circular Dichroism of DNA, Proteins and Chromatin....Pages 67-107
Important Hydrodynamic and Spectroscopic Techniques in the Field of Chromatin Structure....Pages 109-124
Preparation and Analysis of Core Particles and Nucleosomes: A Convenient Method for Studying the Protein Composition of Nucleosomes using Protamine-Release into Triton-Acid-Urea Gels....Pages 125-136
The Interaction of Histones with DNA: Equilibrium Binding Studies....Pages 137-165
Nucleosome Shape and Structure in Solution from Flow Birefringence....Pages 167-185
Scattering and Diffraction by Neutrons and X-Rays in the Study of Chromatin....Pages 187-216
Nuclear Magnetic Resonance Studies of Nucleic Acids and Proteins....Pages 217-249
Techniques for Cytochemical Studies of the Nucleus and its Substructures....Pages 251-264
Chromatin Study in Situ: I — Image Analysis....Pages 265-292
Chromatin Study In Situ : II. Static and Flow Microfluorimetry....Pages 293-322
Chromatin Study In Situ: III. Differential Effects of Feulgen Hydrolysis....Pages 323-339
Scanning and Flow Photometry of Chromosomes....Pages 341-356
Back Matter....Pages xxi-xxx