Chimeric Antigen Receptor T Cells: Development and Production

Front Matter ....Pages i-xi
 CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers  (Virgínia Picanço-Castro, Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Dimas Tadeu Covas)....Pages 1-10
Front Matter ....Pages 11-11
 Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries  (Nestor F. Leyton-Castro, Marcelo M. Brigido, Andrea Q. Maranhão)....Pages 13-26
 Phase I/II Manufacture of Lentiviral Vectors Under GMP in an Academic Setting  (Anindya Dasgupta, Stuart Tinch, Kathleen Szczur, Rebecca Ernst, Nathaniel Shryock, Courtney Kaylor et al.)....Pages 27-60
 Production of Lentivirus for the Establishment of CAR-T Cells  (Marlous G. Lana, Bryan E. Strauss)....Pages 61-67
 Optimized Production of Lentiviral Vectors for CAR-T Cell  (Pablo Diego Moço, Mário Soares de Abreu Neto, Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro)....Pages 69-76
 Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells  (Aline Do Minh, Michelle Yen Tran, Amine A. Kamen)....Pages 77-83
Front Matter ....Pages 85-85
 In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development  (Androulla N. Miliotou, Lefkothea C. Papadopoulou)....Pages 87-117
 Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors  (Feiyan Mo, Maksim Mamonkin)....Pages 119-130
 Generation of CAR+ T Lymphocytes Using the Sleeping Beauty Transposon System  (Leonardo Chicaybam, Luiza Abdo, Martín H. Bonamino)....Pages 131-137
 Platforms for Clinical-Grade CAR-T Cell Expansion  (Amanda Mizukami, Kamilla Swiech)....Pages 139-150
 CAR-T Cell Expansion in a Xuri Cell Expansion System W25  (Trevor A. Smith)....Pages 151-163
 Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform  (Josh Ludwig, Mark Hirschel)....Pages 165-177
Front Matter ....Pages 179-179
 Replication-Competent Lentivirus Analysis of Vector-Transduced T Cell Products Used in Cancer Immunotherapy Clinical Trials  (Kenneth Cornetta, Sue Koop, Emily Nance, Kimberley House, Lisa Duffy)....Pages 181-194
 Immunophenotypic Analysis of CAR-T Cells  (Júlia Teixeira Cottas de Azevedo, Amanda Mizukami, Pablo Diego Moço, Kelen Cristina Ribeiro Malmegrim)....Pages 195-201
 Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies  (Robert D. Schwab, Darel Martínez Bedoya, Tiffany R. King, Bruce L. Levine, Avery D. Posey Jr.)....Pages 203-211
 Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays  (Renata Nacasaki Silvestre, Pablo Diego Moço, Virgínia Picanço-Castro)....Pages 213-222
 Analysis of CAR-Mediated Tonic Signaling  (Hugo Calderon, Maksim Mamonkin, Sonia Guedan)....Pages 223-236
 Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model  (Marcelo de Souza Fernandes Pereira, Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro)....Pages 237-250
 Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors  (Alba Rodriguez-Garcia, Keisuke Watanabe, Sonia Guedan)....Pages 251-271
 Correction to: In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development  (Androulla N. Miliotou, Lefkothea C. Papadopoulou)....Pages C1-C1
Back Matter ....Pages 273-274