Chaperones: Methods and Protocols

Front Matter ....Pages i-xix
 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice  (Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, Nahid F. Mivechi)....Pages 1-22
 Role of Heat Shock Factors in Stress-Induced Transcription  (Ayesha Murshid, Thomas L. Prince, Ben Lang, Stuart K. Calderwood)....Pages 23-34
 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter  (Toshiki Kijima, Takanori Eguchi, Len Neckers, Thomas L. Prince)....Pages 35-45
 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay  (Mikko Taipale)....Pages 47-58
 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay  (Maria A. W. H. van Waarde-Verhagen, Harm H. Kampinga)....Pages 59-74
 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors  (T. A. J. Haystead)....Pages 75-86
 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine  (Jason Davenport, Lakshmi Galam, Robert L. Matts)....Pages 87-96
 Primary Colorectal Cells Culture as a Translational Research Model  (Sheah Lin Lee, Nina Claire Dempsey-Hibbert, Dale Vimalachandran, Terence David Wardle, Paul A. Sutton, John H. H. Williams)....Pages 97-105
 Cell Death and Survival Assays  (Alexander E. Kabakov, Vladimir L. Gabai)....Pages 107-127
 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro  (Benjamin K. Gibbs, Carole Sourbier)....Pages 129-137
 Proteomic Profiling of Hsp90 Inhibitors  (Sudhakar Voruganti, Jake T. Kline, Maurie J. Balch, Janet Rogers, Robert L. Matts, Steven D. Hartson)....Pages 139-162
 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides  (André-Patrick Arrigo)....Pages 163-178
 Nucleotide Exchange Factors for Hsp70 Chaperones  (Heike Rampelt, Matthias P. Mayer, Bernd Bukau)....Pages 179-188
 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast  (Michael Reidy)....Pages 189-198
 Bacterial Hsp90 ATPase Assays  (Joel R. Hoskins, Sue Wickner, Shannon M. Doyle)....Pages 199-207
 Detecting Posttranslational Modifications of Hsp90  (Rebecca A. Sager, Mark R. Woodford, Len Neckers, Mehdi Mollapour)....Pages 209-219
 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)  (Aneliya Yoveva, Ritwick Sawarkar)....Pages 221-231
 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond  (Benjamin J. Lang, Kristina M. Holton, Jianlin Gong, Stuart K. Calderwood)....Pages 233-252
 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors  (Gennady M. Verkhivker)....Pages 253-273
 Computational Analysis of the Chaperone Interaction Networks  (Ashwani Kumar, Kamran Rizzolo, Sandra Zilles, Mohan Babu, Walid A. Houry)....Pages 275-291
 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer  (Francesco Cappello, Everly Conway de Macario, Francesca Rappa, Giovanni Zummo, Alberto J. L. Macario)....Pages 293-305
 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody  (Stefan Stangl, Gemma A. Foulds, Helena Fellinger, Geoffrey J. Pilkington, A. Graham Pockley, Gabriele Multhoff)....Pages 307-320
 Detection and Analysis of Extracellular Hsp90 (eHsp90)  (Stephanie Cortes, Alexander J. Baker-Williams, Mehdi Mollapour, Dimitra Bourboulia)....Pages 321-329
 Molecular Chaperone Receptors  (Ayesha Murshid, Jimmy Theriault, Jianlin Gong, Stuart K. Calderwood)....Pages 331-344
 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy  (Chunqing Guo, John R. Subjeck, Xiang-Yang Wang)....Pages 345-357
 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells  (Desheng Weng, Stuart K. Calderwood, Jianlin Gong)....Pages 359-369
 Hsp70: A Cancer Target Inside and Outside the Cell  (Christophe Boudesco, Sebastien Cause, Gaëtan Jego, Carmen Garrido)....Pages 371-396
 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding  (Marc B. Cox, Jill L. Johnson)....Pages 397-422
 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors  (Akira Yuno, Min-Jung Lee, Sunmin Lee, Yusuke Tomita, David Rekhtman, Brittni Moore et al.)....Pages 423-441
Back Matter ....Pages 443-445