Cellular Regulation by Protein Phosphorylation

Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Signal Integration in Phosphorylase Kinase....Pages 3-16
Physical Approaches to Conformation and Assembly of Biological Macromolecules....Pages 17-28
A Vibrational Raman Spectroscopic Study of Myosin and Myosin - Vanadate Interactions....Pages 29-33
Phosphorylation and the Frequency Encoding of Signal-Induced Calcium Oscillations....Pages 35-39
Front Matter....Pages 41-41
Determination of Phosphorylated Amino Acids in Protein Sequences....Pages 43-50
Back-Phosphorylation — A Sensitive Technique to Study Protein Phosphorylation in the Intact Heart....Pages 51-65
Thermal Transitions in Cardiac Troponin and its Subunits....Pages 67-71
Dynamic Phosphorylation of a Small Chloroplast Protein Exhibiting So Far Undescribed Labelling Properties....Pages 73-77
Identification of Phosphorylation Sites in the Nicotinic Acetylcholine Receptor by Edman Degradation and Mass Spectroscopy LC/MS and LC/MS/MS....Pages 79-84
A Sensitive Colorimetric Assay for Protein Phosphatase Activity....Pages 85-88
Front Matter....Pages 89-89
Protein Kinase Structure & Function: cAMP-Dependent Protein Kinase....Pages 91-105
Expression in E. Coli of Mutated R Subunits of the cAMP-Dependent Protein Kinase from Dictyostelium Discoideum .....Pages 107-111
Expression of the γ-subunit of Phosphorylase b Kinase in Escherichia coli ....Pages 113-117
Conformational and Shape Changes Associated with cAMP- Dependent Protein Kinase....Pages 119-123
Crystallographic Studies of the Catalytic Subunit of cAMP- Dependent Protein Kinase....Pages 125-129
6-Phosphofructo 2-Kinase/Fructose 2, 6-Bisphosphatase: Kinetic Changes Induced by Phosphorylation....Pages 131-135
Enzymes Involved in the Reversible Phosphorylation in Microvessels of the Brain....Pages 137-141
Covalent Modification of Creatine Kinase by ATP: Evidence for Autophosphorylation....Pages 143-147
Two Adjacent Phosphoserines in Bovine, Rabbit and Human Cardiac Troponin I....Pages 149-157
Characterisation of Thylakoid Membrane Protein Kinase by Affinity and Immunological Methods....Pages 159-163
Front Matter....Pages 165-165
A Diversity of Elements in the Protein Kinase C Signal Transduction Pathway....Pages 167-177
“Independent” Protein Kinases: A Challenge to Canons....Pages 179-193
Biphasic Activation of the S6 Kinase: Identification of Signalling Pathways....Pages 195-207
Changes on the Electrophoretic Mobility of CD5 Molecules Induced by PKC-Mediated Phosphorylation....Pages 209-213
Opposing Effects of Protein Kinase C on IgE-Dependent Exocytosis and InsP 3 Formation....Pages 215-219
Proteinphosphorylation in Platelets-Evidence for Increased Protein Kinase C Activity in Essential Hypertension....Pages 221-225
Protein Phosphorylation in Luteal Membrane Fraction....Pages 227-231
Purification of Bovine Brain Protein Kinase C Employing Metal Ion Dependent Properties....Pages 233-237
Cardiac Protein Kinase C Isozymes: Phosphorylation of Phospholamban in Longitudinal and Junctional Sarcoplasmic Reticulum....Pages 239-243
Characterization of the Phosphorylation Sites of 40S Ribosomal Protein S6....Pages 245-249
Front Matter....Pages 251-251
G Protein Oncogenes....Pages 253-260
A New Role for βy-Subunits of G-Proteins?....Pages 261-270
Conditions Favouring Phosphorylation Inhibit the Activation of Adenylate Cyclase in Human Platelet Membranes....Pages 271-275
Front Matter....Pages 277-277
Regulation of Tyrosine Kinases by Tyrosine Phosphorylation....Pages 279-288
pp75: A Novel Tyrosine Phosphorylated Protein that Heralds Differentiation of HL-60 Cells....Pages 289-293
Preliminary Biochemical Studies of a Drosophila Homolog of p60 c-src ....Pages 295-299
Phosphoinositide Kinases and EGF Receptor Activation in Plasma Membranes from A431 Cells....Pages 301-306
Phosphorylation of Synaptophysin by the c-src Encoded Protein Tyrosin Kinase pp60 c-src ....Pages 307-311
Altered Thymocyte Development Induced by Augmented Expression of p56 lck ....Pages 313-317
Front Matter....Pages 319-319
Regulation and Regulatory Role of the Inactive ATP,Mg-Dependent Protein Phosphatase (PP-1I)....Pages 321-329
Front Matter....Pages 319-319
Okadaic Acid from Laboratory Cultures of a Dinoflagellate Alga: Effects on Protein Phosphorylation in C3H10T1/2 Fibroblasts....Pages 331-335
Regulation of Proto-Oncogene Expression and Rate of Protein Synthesis by the Tumor Promoter Okadaic Acid....Pages 337-341
The Use of β-Cyclodextrin in the Purification of Protein Phosphatase G from Rat Liver....Pages 343-347
Characterization of a Human T-cell Protein Tyrosine Phosphatase Expressed in the Baculovirus System....Pages 349-353
Inhibition of Tyrosine Protein Phosphatases from Muscle and Spleen by Nucleic Acids and Polyanions....Pages 355-360
Front Matter....Pages 361-361
Biochemical Regulation of the CDC2 Protein Kinase....Pages 363-373
Molecular and Biochemical Characterization of the Mitogen-Activated S6 Kinase....Pages 375-388
Protein Phosphorylation in the Nervous System....Pages 389-396
Mitotic Control in Mammalian Cells, Positive and Negative Regulation by Protein Phosphorylation....Pages 397-409
Regulation of Eukaryotic Translation by Protein Phosphorylation....Pages 411-419
Regulation of Microfilament Assembly....Pages 421-427
Stimulation of Human DNA Topoisomerase I By Protein Kinase C....Pages 429-433
Protein Phosphorylation in Partially Synchronized Cell Suspension Culture of Alfalfa....Pages 435-439
Phosphorylation of Elongation Factor Tu in Vitro and in Vivo ....Pages 441-445
About a Controversy Concerning the Existence of a Mitogen-Responsive S6-Kinase (Late-Eluting from Deae-Sephacel)....Pages 447-451
Expression of Ferritin Messenger RNA in an in vitro Model of Human CD3-Mediated T-Lymphocyte Activation....Pages 453-457
Relaxation of Smooth Muscle at High Levels of Myosin Light Chain (MLC) Phosphorylation....Pages 459-464
Evidence for Site- and Domain-Specific Phosphorylation of the 145-kDa Neurofilament Subunit In Vivo ....Pages 465-469
Tumor Necrosis Factor-Induced Gene Expression and Cytotoxicity Share a Signal Transduction Pathway....Pages 471-475
Protein Phosphorylation is involved in the Recognition of Pathogen-Derived Signals by Plant Cells....Pages 477-481
Front Matter....Pages 361-361
Effect of membrane modifiers on polyphosphoinositide synthesis in rat heart sarcolemma....Pages 483-487
Phosphorylation and Acylation of the Growth-Related Murine Small Stress Protein P25....Pages 489-493
Back Matter....Pages 495-505