Cell-Free Translation Systems

Front Matter....Pages I-VIII
Front Matter....Pages 1-1
Cell-Free Protein Synthesis....Pages 3-20
Front Matter....Pages 21-21
Improved Batch Translation System Based on E. coli Extract....Pages 23-39
Improved Composition and Energy Supply for Bacterial Batch Systems....Pages 41-52
PURE System....Pages 53-60
Direct Expression of PCR Products in Cell-Free Translation Systems....Pages 61-66
Yeast Cell-Free Translation Systems....Pages 67-78
Poly(A)-Dependent Cell-Free Translation Systems from Animal Cells....Pages 79-88
Front Matter....Pages 89-89
Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors....Pages 91-107
Highly Productive Plant Continuous Cell-Free System....Pages 109-119
Matrix Reactor: A New Scalable Reactor Principle for Cell-Free Protein Expression....Pages 121-128
Front Matter....Pages 129-129
Co-Translational Protein Folding in Prokaryotic and Eukaryotic Cell-Free Translation Systems....Pages 131-140
Expression of an Aggregation-Prone Protein in the RTS 500 System....Pages 141-147
Expression of Soluble Murine Endostatin with the RTS System....Pages 149-156
Cell-Free Expression of Soluble Human Erythropoietin....Pages 157-163
Cell-Free Expression of a 127 kDa Protein: The Catalytic Subunit of Human Telomerase....Pages 165-173
The Expression of Disulfide Bonded Proteins in Cell-Free Protein Expression....Pages 175-179
Front Matter....Pages 181-182
Structural and Functional Compensation by Proteins for the RNA Deficit of Animal Mitochondrial Translation Systems....Pages 183-196
Proficient Target Selection in Structural Genomics by In Vitro Protein Expression on Gateway Recombination Plasmids....Pages 197-202
High-Level Cell-Free Protein Expression from PCR-Generated DNA Templates....Pages 203-210
Optimization of the Translation Initiation Region of Prokaryotic Expression Vectors: High Yield In Vitro Protein Expression and mRNA Folding....Pages 211-217
Front Matter....Pages 181-182
Selective Labeling of Proteins in the RTS 500 System....Pages 219-225
In Vitro Protein Production for Structure Determination with the RTS System....Pages 227-233
Sequence Specific Biotinylation and Purification of Proteins Expressed in the RTS 500 System....Pages 235-246
Synthesis of the His 6-Tagged Recombinant Protein APPC99-His and His-PS1 Using RTS 500....Pages 247-252
Back Matter....Pages 253-259