Cell-Free Protein Expression

Front Matter....Pages I-X
Front Matter....Pages 1-1
Generation of Linear Expression Elements by PCR....Pages 3-7
Rapid Protein Engineering by Expression-PCR....Pages 9-13
Expression-PCR: from Gene Pools to Purified Proteins Within 1 Day....Pages 15-23
High-Throughput Expression PCR Used to Systematically Investigate Regulation of Translation Initiation in an E. coli Cell-Free Expression System....Pages 25-34
Reduction of Primer-Dimer Formation during Generation of Expression Fragments by PCR....Pages 35-39
Front Matter....Pages 41-41
Isotope Labeling of Proteins for NMR Spectroscopy Using Cell-Free Methods....Pages 43-54
Production of a Specifically Labeled Protein in mg Quantities for NMR Analysis....Pages 55-60
 In Situ Mono-Biotinylation of Cell-Free Expressed Proteins Using the AviTag Technology....Pages 61-67
Rapid Generation of Protein Variants and Subsequent Analysis by Surface Plasmon Resonace....Pages 69-79
Incorporation of Fluorescence Labels into Cell-Free Produced Proteins....Pages 81-88
Expression of ‘Tailor-Made’ Proteins via Incorporation of Synthetic Amino Acids by Using Cell-Free Protein Synthesis....Pages 89-98
Front Matter....Pages 99-99
Application of Cell-free Expression Systems to Proteomic Studies....Pages 101-107
 In Vitro Expression of Proteins with Disulfide Bridges and its Application for a High-Throughput Screening System....Pages 109-116
Cell-Free Expression of Proteins Containing Multiple Disulfide Bonds....Pages 117-124
Cell-Free Synthesis of Membrane Proteins on a Preparative Scale....Pages 125-131
Front Matter....Pages 133-139
Using Maltose-Binding Protein Fragment Complementation to Probe Protein-Protein Interactions by Co Expression in the RTS System....Pages 141-141
 In Vitro Translation of KRAB Zinc Finger Transcriptional Repressor Proteins and Their Interaction with Their TIF1β Co-Repressor....Pages 143-149
Optimization of Cell-Free Expression of FAD-Dependent D-Amino Acid Oxidase....Pages 151-157
Expression of Recombinant Chemokine-Like Factor 1 with a Cell-Free Protein Biosynthesis System....Pages 159-164
Front Matter....Pages 165-171
Recombinant Expression of Functional Active MIA (Melanoma Inhibitory Activity) Protein for Mutation Analysis Using the RTS System....Pages 141-141
Cell-Free Expression of the Heterodimeric Protein Penicillin G Amidase in a Functionally Active Form....Pages 173-179
Cell-Free Expression of the His-Tagged Recombinant Prolactin-Like Placenta Protein E Using the RTS 500 System....Pages 181-187
Front Matter....Pages 189-195
Complementary Interaction Between the Central Domain of 18S rRNA and the 5’ Untranslated Region of mRNA Enhances Translation Efficiency in Plants....Pages 197-197
 In Vitro Translation in an Insect-Based Cell-Free System....Pages 199-208
Back Matter....Pages 209-217
....Pages 219-223