Cell Cycle Control Mechanisms And Protocols

1 Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts......Page 2
2 The Plant Cell Cycle......Page 31
3 The C. elegans Cell Cycle......Page 51
4 Developmental Control of Growth and Cell Cycle Progression in Drosophila......Page 69
5 The Xenopus Cell Cycle......Page 95
6 The Mammalian Cell Cycle......Page 113
7 Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle......Page 157
8 Mapping Origins of DNA Replication in Eukaryotes......Page 167
9 In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast......Page 181
10 Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs......Page 189
11 The Cell Cycle and Virus Infection......Page 197
12 Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies......Page 219
13 E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression......Page 239
14 Forkhead (FOX) Transcription Factors and the Cell Cycle......Page 247
15 Measurement of Geminin Activity in Xenopus Egg Extracts......Page 263
16 CDK-Activating Kinases......Page 279
17 Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors......Page 291
18 Measurement of Wee Kinase Activity......Page 299
19 CDC25 Dual-Specificity Protein Phosphatases......Page 329
20 Assaying Cell Cycle Checkpoints......Page 345
21 Polo-Like Kinase-1......Page 355
22 The Ipl1/Aurora Kinase Family......Page 371
23 Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs......Page 383