Cell Culture Engineering VI

Front Matter....Pages i-vi
Cell therapy in kidney failure....Pages 1-8
Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)....Pages 9-17
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination....Pages 19-29
Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line....Pages 31-42
Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them....Pages 43-48
An overview of viral and viral-like agents in cell culture systems....Pages 49-52
New adenovirus vectors for protein production and gene transfer....Pages 53-64
Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue....Pages 65-72
Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth — associated production of a foreign protein, β-galactosidase....Pages 73-80
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy....Pages 81-89
Recombinant insulin-like growth factor-I (IGF-I) production in Super -CHO results in the expression of IGF-I receptor and IGF binding protein 3....Pages 91-99
Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions....Pages 101-109
Regulated multicistronic expression technology for mammalian metabolic engineering....Pages 111-125
Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions....Pages 127-138
Historical reflections on cell culture engineering....Pages 139-145
Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation....Pages 147-155
Population balance model of in vivo neutrophil formation following bone marrow rescue therapy....Pages 157-162
Mammalian cell retention devices for stirred perfusion bioreactors....Pages 163-175
Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells....Pages 177-188
Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions....Pages 189-203
Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures....Pages 205-211
Effects of CO 2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production....Pages 213-227
pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons....Pages 229-235