Branched-Chain Amino Acids

Content: 
Contributors to volume 166
Pages xi-xiv

Preface
Page xv
Robert A. Harris, John R. Sokatch

Volumes in series
Pages xvii,xix-xxxi

[1] Determination of branched-chain amino and keto acids with leucine dehydrogenase Original Research Article
Pages 3-10
Geoffrey Livesey, Patricia Lund

[2] Analysis of phosphorylation sites on branched-chain 2-keto acid dehydrogenase complex Original Research Article
Pages 10-14
Stephen J. Yeaman, Kenneth G. Cook, Andrew P. Bradford, Sheelagh M.A. Jones

[3] High-performance liquid chromatographic separation of (3R)- and (3S)-ОІ-leucine using Marfey's reagent Original Research Article
Pages 14-17
D. John Aberhart, Jacques-Alain Cotting

[4] High-performance liquid chromatographic separation of О±- and ОІ-leucine Original Research Article
Pages 17-19
D. John Aberhart

[5] Quantitative high-performance liquid chromatographic analysis of branched-chain 2-keto acids in biological samples Original Research Article
Pages 20-27
Angela L. Gasking, William T.E. Edwards, Anthony Hobson-Frohock, Marinos Elia, Geoffrey Livesey

[6] Determination of branched-chain 2-hydroxy and 2-keto acids by mass spectrometry Original Research Article
Pages 27-38
Orval A. Mamer, Jane A. Montgomery

[7] Measurement of plasma and tissue levels of branched-chain О±-keto acids by gas-liquid chromatography Original Research Article
Pages 39-46
Pamela L. Crowell, Robert H. Miller, Alfred E. Harper

[8] Determination of methylmalonic acid in biological fluids by mass spectrometry Original Research Article
Pages 47-55
Jane A. Montgomery, Orval A. Mamer

[9] Analysis of acyl-coenzyme A esters in biological samples Original Research Article
Pages 55-70
Barbara E. Corkey

[10] Determination of short-chain coenzyme A compounds by reversed-phase high-performance liquid chromatography Original Research Article
Pages 70-79
M. Todd King, Paul D. Reiss, Neal W. Cornell

[11] Radiochemical high-performance liquid chromatography methods for the study of branched-chain amino acid metabolism Original Research Article
Pages 79-92
Kim Bartlett, Anthony G. Causey

[12] Synthesis of О±-isopropylmalate, ОІ-isopropylmalate, and dimethylcitraconate Original Research Article
Pages 92-96
John V. Schloss, Ron Magolda, Mark Emptage

[13] Utilization of sulfometuron methyl, an acetolactate synthase inhibitor, in molecular biological and metabolic studies of plants and microbes Original Research Article
Pages 97-107
Robert A. LaRossa, Tina K. Van Dyk

[14] Antibodies against branched-chain О±-keto acid dehydrogenase proteins for use in defining human mutations and gene isolation Original Research Article
Pages 108-114
Dean J. Danner, Louis J. Elsas, Stuart Litwer

[15] Inhibition of branched-chain О±-keto acid dehyrogenase kinase by О±-chloroisocaproate Original Research Article
Pages 114-123
Robert A. Harris, Martha J. Kuntz, Ronald Simpson

[16] Measurement of relative carbon flux in О±- and ОІ-keto pathways of leucine metabolism Original Research Article
Pages 127-130
J. Michael Poston

[17] Assay of leucine 2,3-aminomutase Original Research Article
Pages 130-135
J. Michael Poston

[18] Enzyme assays with mutant cell lines of maple syrup urine disease Original Research Article
Pages 135-146
David T. Chuang, Rody P. Cox

[19] Assays for E1 and E2 components of the branched-chain keto acid dehydrogenase complex Original Research Article
Pages 146-154
David T. Chuang

[20] Mutant isovaleryl-CoA dehydrogenase in isovaleric acidemia cells: Assay of activity and molecular characterization Original Research Article
Pages 155-166
Yasuyuki Ikeda, Kay Tanaka

[21] Assay of branched-chain О±-keto acid dehydrogenase kinase in mitochondrial extracts and purified branched-chain О±-keto acid dehydrogenase complexes Original Research Article
Pages 166-175
Joseph Espinal, Mark Beggs, Philip J. Randle

[22] Assay of total complex and activity state of branched-chain О±-keto acid dehydrogenase complex and of activator protein in mitochondria, cells, and tissues Original Research Article
Pages 175-189
Philip A. Patston, Joseph Espinal, Mark Beggs, Philip J. Randle

[23] Determination of activity and activity state of branched-chain О±-keto acid dehydrogenase in rat tissues Original Research Article
Pages 189-201
Gary W. Goodwin, Bei Zhang, Ralph Paxton, Robert A. Harris

[24] Estimation of branched-chain О±-keto acid dehydrogenase activation in mammalian tissues Original Research Article
Pages 201-213
Kevin P. Block, R. Paul Aftring, Maria G. Buse, Alfred E. Harper

[25] Assay of 3-methylglutaconyl-CoA hydratase Original Research Article
Pages 214-218
Kenneth M. Gibson

[26] Assay of 3-hydroxy-3-methylglutaryl-CoA lyase Original Research Article
Pages 219-225
Kenneth M. Gibson

[27] Permeabilized cell and radiochemical assays for ОІ-isopropylmalate dehydrogenase Original Research Article
Pages 225-229
Lillie L. Searles, Joseph M. Calvo

[28] Rapid assay of acetolactate synthase in permeabilized bacteria Original Research Article
Pages 230-233
Julius H. Jackson

[29] Assay of products of acetolactate synthase Original Research Article
Pages 234-240
Natan Gollop, Ze'ev Barak, David M. Chipman

[30] Detection of the acetolactate synthase isozymes I and III of escherichia coli K12 Original Research Article
Pages 241-244
Maurilio De Felice, Giovanna Griffo, Carmine T. Lago, Danila Limauro, Ezio Ricca

[31] Transport of branched-chain amino acids in escherichia coli Original Research Article
Pages 245-251
Tammy K. Antonucci, Dale L. Oxender

[32] Transport of branched-chain amino acids and their corresponding 2-keto acids by mammalian cells Original Research Article
Pages 252-260
Michael S. Kilberg, Mary E. Handlogten

[33] Leucine-tRNA ligase complexes Original Research Article
Pages 260-265
Arnold Hampel, Richard Tritz

[34] Purification of branched-chain-amino-acid aminotransferase from pig heart Original Research Article
Pages 269-274
T.K. Korpela

[35] Pancreatic branched-chain-amino-acid aminotransferase Original Research Article
Pages 275-281
Ryo Kido

[36] Isolation and characterization of leucine dehydrogenase from bacillus subtilis Original Research Article
Pages 282-288
Geoffrey Livesey, Patricia Lund

[37] Purification and assay of О±-ketoisocaproate dioxygenase from rat liver Original Research Article
Pages 288-297
P.J. Sabourin, L.L. Bieber

[38] Isolation of branched-chain О±-keto acid dehydrogenase as active complex from bovine liver Original Research Article
Pages 298-302
Dean J. Danner, Sue C. Heffelfinger

[39] Purification, resolution, and reconstitution of branched-chain 2-keto acid dehydrogenase complex from bovine kidney Original Research Article
Pages 303-308
Kenneth G. Cook, Stephen J. Yeaman

[40] Branched-chain О±-keto acid dehydrogenase complex from bovine kidney Original Research Article
Pages 309-312
Flora H. Pettit, Lester J. Reed

[41] Branched-chain О±-keto acid dehydrogenase and its kinase from rabbit liver and heart Original Research Article
Pages 313-320
Ralph Paxton

[42] Branched-chain О±-keto acid dehydrogenase phosphatase and its inhibitor protein from bovine kidney Original Research Article
Pages 321-329
Zahi Damuni, Lester J. Reed

[43] Isolation and properties of the branched-chain 2-keto acid and pyruvate dehydrogenase multienzyme complex from Bacillus subtilis Original Research Article
Pages 330-342
Richard N. Perham, Peter N. Lowe

[44] Purification of branched-chain keto acid dehydrogenase and lipoamide dehydrogenase-valine from Pseudomonas Original Research Article
Pages 342-350
John R. Sokatch

[45] Cloning of genes for branched-chain keto acid dehydrogenase in Pseudomonas putida Original Research Article
Pages 350-359
P.J. Sykes, John R. Sokatch

[46] 2-methyl branched-chain acyl-CoA dehydrogenase from rat liver Original Research Article
Pages 360-373
Yasuyuki Ikeda, Kay Tanaka

[47] Isovaleryl-CoA dehydrogenase from rat liver Original Research Article
Pages 374-389
Yasuyuki Ikeda, Kay Tanaka

[48] Purification of methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa PAO Original Research Article
Pages 389-393
Kenneth Hatter, John R. Sokatch

[49] d-methylmalonyl-CoA hydrolase Original Research Article
Pages 393-400
Robin J. Kovachy, Sally P. Stabler, Robert H. Allen

[50] dl-methylmalonyl-CoA racemase from rat liver Original Research Article
Pages 400-406
Sally P. Stabler, Robert H. Allen

[51] l-Methylmalonyl-CoA mutase from human placenta Original Research Article
Pages 407-414
J. Fred Kolhouse, Sally P. Stabler, Robert H. Allen

[52] О±-Isopropylmalate synthase from yeast Original Research Article
Pages 414-423
Gunter B. Kohlhaw

[53] Isopropylmalate dehydratase from yeast Original Research Article
Pages 423-429
Gunter B. Kohlhaw

[54] ОІ-Isopropylmalate dehydrogenase from yeast Original Research Article
Pages 429-435
Gunter B. Kohlhaw

[55] Purification and assays of acetolactate synthase I from Escherichia coli K12 Original Research Article
Pages 435-445
Lillian Eoyang, Philip M. Silverman

[56] Acetolactate synthase isozyme II from Salmonella typhimurium Original Research Article
Pages 445-454
John V. Schloss, Drew E. Van Dyk

[57] Acetolactate synthase isozyme III from Escherichia coli Original Research Article
Pages 455-458
Ze'ev Barak, Joseph M. Calvo, John V. Schloss

[58] Amino acid control of intracellular protein degradation Original Research Article
Pages 461-476
Glenn E. Mortimore, A. Reeta Pösö

[59] Use of rat hindquarter preparations in studies of branched-chain amino acid metabolism Original Research Article
Pages 476-483
E. Jack Davis, Sung-Hee Cho Lee, Øystein Spydevold, Jon Bremer

[60] Measurement of branched-chain О±-keto acid dehydrogenase flux rates in perfused heart and liver Original Research Article
Pages 484-497
David S. Lapointe, Ellen Hildebrandt, Denis B. Buxton, Tarun B. Patel, Parvan P. Waymack, Merle S. Olson

Author index
Pages 499-514

Subject index
Pages 515-534