Biophysical Tools for Biologists, Volume One: In Vitro Techniques

Content: 
Binding: A Polemic and Rough Guide Review Article
Pages 1,3-23
Nichola C. Garbett, Jonathan B. Chaires

Linked Equilibria in Regulation of Transcription Initiation Review Article
Pages 25-52
Dorothy Beckett

Biosensor‐Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions Review Article
Pages 53-77
Farial A. Tanious, Binh Nguyen, W. David Wilson

Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions Review Article
Pages 79-113
Matthew W. Freyer, Edwin A. Lewis

Differential Scanning Calorimetry Review Article
Pages 115-141
Charles H. Spink

Analytical Ultracentrifugation: Sedimentation Velocity and Sedimentation Equilibrium Review Article
Pages 143-179
James L. Cole, Jeffrey W. Lary, Thomas P. Moody, Thomas M. Laue

Determination of Membrane Protein Molecular Weights and Association Equilibrium Constants Using Sedimentation Equilibrium and Sedimentation Velocity Review Article
Pages 181-211
Nancy K. Burgess, Ann Marie Stanley, Karen G. Fleming

Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods Review Article
Pages 213-242
Natasha Shanker, Susan L. Bane

Applications of Fluorescence Anisotropy to the Study of Protein–DNA Interactions Review Article
Pages 243-262
Vince J. LiCata, Andy J. Wowor

Circular Dichroism and Its Application to the Study of Biomolecules Review Article
Pages 263-293
Stephen R. Martin, Maria J. Schilstra

Protein Folding and Stability Using Denaturants Review Article
Pages 295-325
Timothy O. Street, Naomi Courtemanche, Doug Barrick

Hydrodynamic Modeling: The Solution Conformation of Macromolecules and Their Complexes Review Article
Pages 327-373
Olwyn Byron

X‐Ray and Neutron Scattering Data and Their Constrained Molecular Modeling Review Article
Pages 375-423
Stephen J. Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert, Patricia B. Furtado

Structural Investigations into Microtubule‐MAP Complexes Review Article
Pages 425-444
Andreas Hoenger, Heinz Gross

Rapid Kinetic Techniques Review Article
Pages 445-477
John F. Eccleston, Stephen R. Martin, Maria J. Schilstra

Mutagenic Analysis of Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example Review Article
Pages 479-515
George J. Turner

Quantifying DNA–Protein Interactions by Single Molecule Stretching Review Article
Pages 517-540
Mark C. Williams, Ioulia Rouzina, Richard L. Karpel

Isotopomer‐Based Metabolomic Analysis by NMR and Mass Spectrometry Review Article
Pages 541-588
Andrew N. Lane, Teresa W.‐M. Fan, Richard M. Higashi

Following Molecular Transitions with Single Residue Spatial and Millisecond Time Resolution Review Article
Pages 589-615
Inna Shcherbakova, Somdeb Mitra, Robert H. Beer, Michael Brenowitz

Methods and Applications of Site-Directed Spin Labeling EPR Spectroscopy Review Article
Pages 617-658
Candice S. Klug, Jimmy B. Feix

Fluorescence Correlation Spectroscopy and Its Application to the Characterization of Molecular Properties and Interactions Review Article
Pages 659-678
Hacène Boukari, Dan L. Sackett

A Practical Guide on How Osmolytes Modulate Macromolecular Properties Review Article
Pages 679-735
Daniel Harries, Jörg Rösgen

Stupid Statistics! Review Article
Pages 737,739-780
Joel Tellinghuisen

Nonlinear Least‐Squares Fitting Methods Review Article
Pages 781-805
Michael L. Johnson

Methods for Simulating the Dynamics of Complex Biological Processes Review Article
Pages 807-842
Maria J. Schilstra, Stephen R. Martin, Sarah M. Keating

Computational Methods for Biomolecular Electrostatics Review Article
Pages 843-870
Feng Dong, Brett Olsen, Nathan A. Baker

Ligand Effects on the Protein Ensemble: Unifying the Descriptions of Ligand Binding, Local Conformational Fluctuations, and Protein Stability Review Article
Pages 871-891
Steven T. Whitten, Bertrand E. García‐Moreno, Vincent J. Hilser

Molecular Modeling of the Cytoskeleton Review Article
Pages 893-910
Xiange Zheng, David Sept

Mathematical Modeling of Cell Migration Review Article
Pages 911-937
Anders E. Carlsson, David Sept

Index
Pages 939-964

Contents of Previous Volumes
Pages 965-972

Preface
Pages XIX-XXII
John J. Correia, H. William Detrich III

Dedication
Page XXIII

Contributors to Volume 84
Pages XV-XVIII