Biomolecules and Corrosion

Contents
Preface
Acknowledgments
Chapter 1
Corrosion: An Overview
	Abstract
	Introduction
	Microbiologically Influenced Corrosion (MIC)
	Mechanisms
		Cathodic Depolarization by Hydrogenase
		Iron Sulphides (King’s Mechanism)
		Volatile Compound of Phosphorous
		Anodic Depolarization
		Fe-Binding Exopolymers
		Sulphide and Hydrogen-Induced Stress Corrosion Cracking (SCC)
		Role of Sulphides
		Three Stages Mechanism (Romero Mechanism)
		Biocatalytic Cathodic Sulphate Reduction (BCSR)
	Economical Loss from Corrosion
Chapter 2
The Role of Biofilm in Corrosion
	Abstract
	Introduction
	Biofilm in Industries and Their Significance
	Places Where Biofilm Forms
	Organisms Involved in Biofilm Formation
	Mechanisms of Biofilm Formation
	Economical Loss Due to Biofilm Formation
Chapter 3
Microbial Diversity and Their Importance
	Abstract
	Introduction
	Microbial Diversity
	Microorganisms Involved in the Corrosion Process
	Iron-Oxidizing Bacteria
	Iron-Reducing Bacteria
	Manganese-Oxidizing Bacteria
	Manganese-Reducing Bacteria
	Sulphur-Reducing or Oxidizing Bacteria
Chapter 4
Techniques to Determine  the Microbial Diversity
	Abstract
	Introduction
	Morphological Characterization of Bacteria
	Biochemical Characterization
		Fatty Acid Methyl Ester (FAME) Analysis
	Molecular Methods to Determine Microbial Diversity
		Molecular-Based Approach for Analyzing Microbial Diversity
		Amplified Ribosomal Restriction DNA Analysis (ARDRA)
		Random Amplification of Polymorphic DNA (RAPD)
		REP-PCR (BOX Element)
		Ribosomal Intergenic Spacer Analysis (RISA)/ Automated Intergenic Spacer Analysis (ARISA)
		Single-Strand Conformation Polymorphisms (SSCP)
		PCR-Independent Approaches
		DNA-Reassociation Kinetics and DNA: DNA Hybridization
		DNA Microarrays
		Reverse Sample Genome Probing (RSGP)
		Phylogenetic Analysis and 16S rDNA Gene Sequencing
		Next‐Generation Sequencing
		Using the Flow Cytometry Method to Determine Microbial Diversity
		Using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI)-Based Method to Determine Microbial Diversity
Chapter 5
Extracellular Polymeric Substances  and Corrosion
	Abstract
	Introduction
		EPS: An Overview
	Source of EPS
	EPS in the Corrosion Process
	Techniques to Determine EPS
Chapter 6
Proteins in Corrosion
	Abstract
	Introduction
		Proteins: An Overview
		Enzymes: An Overview
	Role of Proteins and Enzymes in the Corrosion Process
	Techniques to Determine Enzymes in Corrosion
		Enzyme-Influenced Oxygen Reduction
		Pivotal Role of Hydrogen Peroxide
		Enzymes Producing H2O2: Oxidases
		Microbial-Induced Deposition of Mn Oxides
Chapter 7
Role of Lipids in Corrosion
	Abstract
	Introduction
		Lipids: An Overview
	Role of Lipids in Corrosion Process
	Sample Preparation
	Separation and Analysis by Chromatography
	Lipid Fractions by TLC
	Fatty Acid Methyl Esters by GC
	Chemical Techniques
Chapter 8
DNA or eDNA (Environmental DNA) in Corrosion Process
	Abstract
	Introduction
		DNA or eDNA
	eDNA: A Source For Detection of New Species
	eDNA in the Corrosion Process
Chapter 9
Technique to Identify Biomolecules or Biofilm in Corrosion
	Abstract
	Introduction
	Confocal Laser Scanning Microscopy (CLSM)
	Determination of Dry Weight and Total Carbohydrate Levels
	Analysis of Metal Ion Leaching and Accumulation by Biofilm
	Microscopic Analysis of Biofilm
		SEM and Energy-Dispersive X-Ray Analysis
		Scanning Electron Microscopy (SEM) Analysis
		Weight Loss of Carbon Steel (Corrosion Rates)
	Identification of Biofilm Bacteria
Chapter 10
Omics Approach in Biocorrosion
	Abstract
	Omics Approach
	Metabolomics: An Approach
	Metabolic Fingerprinting
	Metabolomics in Biocorrosion
	Analysis of Metabolic Foot Printing
References
Index
About the Authors
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