Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987

Front Matter....Pages ii-xxv
Front Matter....Pages 1-1
The Discovery of Enzymatic Transamination and its Biological Significance....Pages 3-10
Professor Alexander E. Braunstein; Some Reminiscences....Pages 11-13
Closing Remarks....Pages 15-17
Front Matter....Pages 19-19
Genes, Biosynthesis, and Intracellular Processing of the Iso-Enzymes of Aspartate Aminotransferase....Pages 21-29
Structure-Function Relation of the Presequence of a Precursor to Pig Mitochondrial Aspartate Aminotransferase....Pages 31-34
Molecular Cloning and in Vivo Expression of the Precursor to Rat Mitochondrial Aspartate Aminotransferase....Pages 35-38
Rat Cytosolic Aspartate Aminotransferase; Molecular Cloning of cDNA and Expression in E . Coli ....Pages 39-42
D-Amino Acid Aminotransferase from a Thermophile, Bacillus SP. YM-1: Enzymological Properties, Cloning of the Gene, and the Amino Acid Sequence....Pages 43-46
The Subunit Structure of Porcine Ornithine Aminotransferase and Mechanism of Proteolytic Modification of its Putative Precursors in Mitochondria....Pages 47-50
Hepatic Systems Degrade Tyrosine Aminotransferase....Pages 51-54
Demonstration of Immunochemical Cross-Reactivity of Dopa Decarboxylase and Histidine Decarboxylase Using Antibodies Against the Two Enzymes....Pages 55-58
Mechanistic Analysis of the Aspartate Aminotransferase Active Site Mutants—Y70F, K258A, and R292D....Pages 59-67
Mutation of Lysine 258 to Arginine in the Active Site of Aspartate Aminotransferase....Pages 69-72
Bacterial Tryptophan Synthase: A Multienzyme Complex Which Contains Pyridoxal Phosphate....Pages 73-81
Site-Specific Mutations in E. coli Maltodextrin Phosphorylase With Differential Effects on Substrate Binding and Catalysis....Pages 83-86
Front Matter....Pages 87-87
Spatial Aspects of Catalysis by Mitochondrial Aspartate Aminotransferase....Pages 89-98
Recent Studies on Mitochondrial Aspartate Aminotransferase: Structure & Mechanism....Pages 99-102
X-Ray Crystallographic, 19 F-NMR and UV-Visible Spectroscopic Studies of Pig Heart Cytosolic Aspartate Aminotransferase....Pages 103-110
Studies on the Inactivated Coenzyme in the Beta Subform of Cytosolic Aspartate Aminotransferase....Pages 111-113
Studies on the Mechanism of Action of Aspartate Aminotransferase (AspAT) by Cryoenzymological and Isotope Techniques....Pages 115-123
Front Matter....Pages 87-87
Aspartate Aminotransferase from the Thermoacidophile Archaebacterium Sulfolobus Solfataricus ....Pages 125-128
Coenzyme Reorientations in the Active Sites of Aspartate Aminotransferase Isoenzymes....Pages 129-133
Phosphorus-31 Nuclear Magnetic Resonance Studies on Apoaspartate Aminotransferase Reconstituted with Cofactor Analogues....Pages 135-138
Fourier Transform Infrared Spectroscopic Analysis of Phosphate Bound to the Aspartate Aminotransferase Isozymes....Pages 139-142
Carboxylate Inhibition of Apoaspartate Aminotransferase....Pages 143-146
The Reversible Oxidation of Vicinal SH Groups in 4-Aminobutyrate Aminotransferase. Probes of Conformational Changes.....Pages 147-152
Branched-Chain Amino Acid Aminotransferase from Escherichia Coli....Pages 153-156
Crystallographic and Solution Studies on Phosphoserine Aminotransferase (PSAT) from E.coli ....Pages 157-160
Studies on Liver Gaba-Transaminase....Pages 161-164
A Novel Aminotransferase Concerned with the Metabolism of Dimethylarginines in Rats....Pages 165-168
Front Matter....Pages 169-169
Chemical Probes of Substrate Channeling in The E. Coli Tryptophan Synthase α 2 β 2 Bienzyme Complex....Pages 171-181
Phosphorus-31 NMR Study of Pyridoxal 5’-Phosphate Binding to Escherichia Coli Tryptophan Synthase Modified by Limited Proteolysis....Pages 183-186
Glutamate Decarboxylase: Preliminary Crystallographic Data....Pages 187-190
Analysis of the Glutamate Decarboxylase Reaction by Membrane Inlet Mass Spectrometry....Pages 191-194
Proteolytic Cleavage of Pig Kidney Dopa Decarboxylase....Pages 195-198
The Reaction of Dopa Decarboxylase With 5’-Hydroxytryptophan Isomers....Pages 199-202
Sequence Studies on Vitamin B6-Dependent Enzymes: Serine Hydroxymethyltransferase and Glutamate Decarboxylase....Pages 203-206
Stereochemistry of the Threonine Cleavage Complex....Pages 207-210
The Substrate Specificity of Tyrosine Phenol-Lyase During the Different Stages of Enzymatic Process....Pages 211-220
Structural and Catalytic Properties of Citrobacter Intermedius Tyrosine Phenol-Lyase....Pages 221-224
Front Matter....Pages 169-169
L-Methionine γ-Lyase: Its Essential Cysteine Residues....Pages 225-228
Diaminopropionate Ammonia-Lyase Of Salmonella Typhimurium ....Pages 229-232
Interactions of Tryptophanase With Substrate Analogues....Pages 233-237
S-Carboxymethylcysteine Synthase From Escherichia Coli ....Pages 239-241
Copper-Containing Amine Oxidases (Ec 1.4.3.6) Have Covalently-Bound PQQ and not PLP as Organic Cofacior....Pages 243-252
Front Matter....Pages 253-253
The Role of the Pyridoxal Phosphate in Glycogen Phosphorylase b ....Pages 255-265
Phosphorylase Isozymes of Higher Plants....Pages 267-276
The Polarized Single Crystal Electronic Absorption Spectrum of Phosphorylase b ....Pages 277-286
Crystallization and Structure Determination of Potato Phosphorylase At 3 Å Resolution....Pages 287-290
Ouaternary Structure and Regulatory Properties of Phosphorylase Kinase from Avian Skeletal Muscle....Pages 291-294
Purification and Properties of Smooth Muscle Phosphorylase from Bovine Stomach....Pages 295-298
Thermodynamic Study of the Binding of Amp to Glycogen Phosphorylase a and b ....Pages 299-302
Front Matter....Pages 303-303
Enzyme-Activated Inhibitors of Bacterial D-Amino Acid Transaminase as Antimicrobial Agents....Pages 305-315
Selective Inhibition of PLP-Dependent Enzymes by Hydroxylamine Derivatives....Pages 317-320
On the Mechanism of Ornithine Decarboxylase Inhibition by 3-Aminooxypropylamine....Pages 321-324
Kinetic Studies on Binding of Mitochondrial and Cytosolic Aspartate Aminotransferases with Suicide Substrate, Gostatin....Pages 325-328
Inactivation of Bacterial ω-Amino Acid: Pyruvate Transaminase with Se -Hypotaurine....Pages 329-332
Absence of Stereospecificity in the Suicide Inhibition of Ornithine Decarboxylase....Pages 333-336
Enzyme-Activated Irreversible Inhibition of Bacterial and Plant Arginine Decarboxylase, by Either Substrate or Product Analogues....Pages 337-340
Cystathionine γ-Synthase and β-Cystathionase have Common Peptide Sequences of the Active Sites and are Inhibited Similarly by Aminoethoxyvinylglycine....Pages 341-344
Front Matter....Pages 345-345
Micellar Catalysis of Pyridoxal-Dependent Reactions....Pages 347-352
A New Approach to the Study Of Pyridoxal-5′-Phosphate Schiff Bases....Pages 353-358
Enthalpy, Entropy and Gibbs Energy Changes Associated with the Ionization of Vitamin B 6 Molecules in Water-Dioxane Systems....Pages 359-362
NMR Detection of a Delocalized Carbanion Intermediate in the Transamination Reactions of the Metal Chelates of Schiff Bases of Vitamin B6....Pages 363-366
Copper(II) Chelates of an Intermediate Species Absorbing in the 500-nm Region....Pages 367-370
Co-Ordination Compounds of Pyridoxol with Chromium and Manganese....Pages 371-374
A Novel Method to Study the Topography of Transmembrane Proteins Using Pyridoxal 5-Phosphate....Pages 375-378
Front Matter....Pages 379-379
Glutamine and Asparagine Transaminases....Pages 381-390
Rat Aspartate Aminotransferase: Regulation of its mRNA and its Contribution to Gluconeogenesis....Pages 391-394
Vitamin B6 Deficiency Enhances End-Organ Sensitivity to Steroid Hormones....Pages 395-398
Partial Purification and Characterization of Vitamin B 6 -P Phosphatases from Human Erythrocytes....Pages 399-402
Pyriloxine Uptake by Proximal Tubular Epithelial Cells Isolated from Rat Kidney....Pages 403-406
Influence of Vitamin B 6 Nutritional Status upon Plasma Kinetics of PLP after I.V. Dosing to Rats....Pages 407-410
Vitamin B 6 Status of Finnish and Dutch Elderly....Pages 411-416
Metabolism of Vitamin B6 by Human Subjects with and without Normal Liver Function....Pages 417-420
Vitamin B 6 Supplementation in Juvenile Neuronal Ceroid Lipofuscinosis Patients....Pages 421-424
Bacterial Methionine Gamma-Lyase as a Possible Antitumor Agent....Pages 425-428
A New Approach to the Pathogenetic Treatment of Psoriasis Using Coenzyme Inhibitors....Pages 429-432
Front Matter....Pages 433-433
Enantiomer Synthesis of Amino Acids with Pyridoxal Enzymes....Pages 435-444
Vitamin B 6 -Dependent Reactions in Organic Solvents....Pages 445-448
Front Matter....Pages 433-433
α-Amino-ε-Caprolactam Racemase for L-Lysine Production....Pages 449-452
Enzymatic Production of L-Cysteine from DL-2-Aminothiazoline- 4-Carboxylic Acid....Pages 453-456
Industrial Production of L-Tryptophan from Indole and DL-Serine with Two PLP-Dependent Enzymes....Pages 457-460
Back Matter....Pages 461-469