Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular

Front Matter....Pages i-xiv
Front Matter....Pages 1-1
The Use of HPLC in Studies of the Stereoselective Metabolism of Prostaglandin D 2 ....Pages 3-13
Analysis of Eicosanoids by GC-MS/MS....Pages 15-21
Quantitative Determination of Several Prostaglandins and Thromboxane B 2 in the Isolated Perfused Rat Brain....Pages 23-27
Analytical Methods for [ 3 H]-Enisoprost, an Anti-Secretory PGE 1 Analogue, and its Metabolites....Pages 29-35
Identification of a New Metabolite of Leukotriene B 4 by RP-HPLC and GC-MS Analysis....Pages 37-41
Radioimmunoassay of Eicosanoids: Its Application in the Development of Anti-Inflammatory and Analgesic Drugs....Pages 43-50
Front Matter....Pages 51-51
Developing Methods for a Thromboxane Synthetase Inhibitor and a Prostacyclin Analogue in Biological Fluids: Problems and Some Solutions....Pages 53-55
The Stereochemical Analysis of Monohydroxyfatty Acids, Particularly 12-Hydroxy-5,8,10,14-Eicosatetraenoic Acid....Pages 57-63
Front Matter....Pages 65-65
Analysis of the Actions of Anti-Inflammatory Drugs, with Particular Reference to Effects on Interleukins and Leucocytes....Pages 67-76
Immunoassay of Beclomethasone 17,21-Dipropionate and Metabolites....Pages 77-86
HPLC Analysis of Anti-Inflammatory Agents (Non-Steroidal and Steroidal)....Pages 87-95
Analytical Approaches Adopted during the Development of Flurbiprofen....Pages 97-105
The Assay of Diclofenac and Metabolites: A Review....Pages 107-114
Enantiomeric Analysis of 2-Phenylpropionic Acid NSAID’s in Biological Fluids by HPLC....Pages 115-125
Front Matter....Pages 127-127
The Determination of Suprofen in Biological Fluids....Pages 129-132
Front Matter....Pages 133-133
Analysis of Angiotensin-Converting Enzyme Inhibitors in Biological Fluids....Pages 135-142
The Analysis of ACE Inhibitors by Immunoassay....Pages 143-148
The Use of Stable-Isotope Methodology in Pharmacokinetic Studies Involving Flunarizine....Pages 149-155
Front Matter....Pages 157-157
Assay Problems with Nifedipine....Pages 159-161
Detection of the Metabolites of Some Dihydropyridine-Type Calcium Antagonists in Urine and Plasma....Pages 163-166
Front Matter....Pages 167-167
The Study of Chiral Cardiovascular Drugs: Analytical Approaches and Some Pharmacological Consequences....Pages 169-177
Development of a Chiral Capillary GC Method for the Quantitation of the Enantiomers of Cromakalim in Biological Fluids....Pages 179-183
The Determination of Plasma Amiodarone and Desethylamiodarone by HPLC....Pages 185-191
The Assay of Hydralazine: A Review....Pages 193-200
Approaches to the Analysis of Two Cardiovascular Drugs in Plasma....Pages 201-206
Front Matter....Pages 207-207
Quantitative Analysis of Flesinoxan in Plasma and Urine at the pg/ml Level Using GC-ECD....Pages 209-212
Assay of Nitroglycerin and Metabolites by Capillary GC....Pages 213-214
HPLC-Fluorescence Method for the Determination of the New β 1 -Adrenoreceptor Blocking Agent Nebivolol in Human Plasma....Pages 215-222
Front Matter....Pages 223-223
The Routine Use, and Column-Stability Implications, of Several Column-Switching HPLC Methods for Determining Drugs in Plasma and Urine....Pages 225-233
Micellar Liquid Chromatography....Pages 235-244
Determination of Nefopam and its Desmethyl Metabolite in Biological Fluids by Paired-Ion RP-HPLC....Pages 245-250
An Assay for Idaverine in Plasma and Urine at Picogram Level....Pages 251-255
Pitfalls in the Enantioselective Analysis of Chiral Drugs....Pages 257-261
Front Matter....Pages 263-263
Chiral Differences in the Disposition of the Quaternary Anticholinergic Drug Oxyphenonium Bromide....Pages 265-268
Quantitation of Danazol in Serum Using HPLC Column Switching with UV Detection....Pages 269-269
Simultaneous Determination of Etretinate, Acitretin and 13- cis -Acitretin in Plasma by Gradient-HPLC Using Automated Column Switching....Pages 271-272
Analytical Consequences of Potent Drugs....Pages 273-276
Optimization of Capillary GC Conditions for the Analysis of Psychotropic Drugs at Subnanogram Concentrations in Plasma....Pages 277-278
Comparison of an Enzyme Method with a Capillary GC Method for Red Blood Cell Sorbitol Assay....Pages 279-281
The Stability of Antineoplastic Vinca Alkaloids in Plasma and Urine....Pages 283-288
Front Matter....Pages 263-263
Solid-Phase Extraction: Personal Experiences....Pages 289-294
The Use of Secondary Ionic Interactions for the Solid-Phase Extraction of Some ‘β-Blocking’ Drugs on C-18 Bonded Silica....Pages 295-298
Application of Solid-Phase Extraction Techniques to the Analysis of Basic and Acidic Drugs in Biological Fluids....Pages 299-300
The Determination of Drug Protein Binding by HPLC Using a Chemically Bonded Bovine-Albumin Stationary Phase....Pages 301-303
Retention Mechanisms in Reversed-Phase HPLC....Pages 305-309
Front Matter....Pages 311-311
Advances in Instrumentalized TLC....Pages 313-320
The Use of Fluorescence and Chemiluminescence Techniques for Sensitive and Selective Detection in HPLC....Pages 321-338
LC-MS and LC-MS-MS for Biomedical Analyses....Pages 339-351
The Structure Elucidation of Drug Metabolites Using Thermospray LC-MS and LC-MS-MS....Pages 353-359
Front Matter....Pages 361-361
An Approach to Metabolite Sample Preparation for Mass Spectrometry to Aid the Spectroscopist....Pages 363-365
Application of NMR and Mass Spectrometry to the Study of Drug Hydroxylation....Pages 367-369
Urinanalysis by 1 H NMR: Application to Cephaloridine-Treated Rats....Pages 371-374
Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo ....Pages 375-381
 19 F NMR Studies of the Metabolism of Trifluoromethylaniline....Pages 383-388
NMR as an Aid in Studying N -Alkylformamides and Metabolites....Pages 389-392
Flexible Versus Dedicated Automation....Pages 393-396
Design of and First Experiments with a Bioluminescence Detector for HPLC....Pages 397-399
A System Approach to Immunoassay Using Enhanced Luminescence....Pages 401-404
Back Matter....Pages 405-415