Basic Science Methods for Clinical Researchers

Content: 
Front-matter,Copyright,Dedication,List of Contributors,Foreword,Preface,About This BookEntitled to full textChapter 1 - The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR, Pages 1-18, Mehdi Jalali, Justyna Zaborowska, Morteza Jalali
Chapter 2 - Methods of Cloning, Pages 19-39, Alessandro Bertero, Stephanie Brown, Ludovic Vallier
Chapter 3 - Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos, Pages 41-56, Martyna Lukoseviciute, Robert Lea, Shoko Ishibashi, Enrique Amaya
Chapter 4 - Microarrays: An Introduction and Guide to Their Use, Pages 57-76, Frederick D. Park, Roman Sasik, Tannishtha Reya
Chapter 5 - Analysis of Human Genetic Variations Using DNA Sequencing, Pages 77-98, Gregory A. Hawkins
Chapter 6 - Western Blot, Pages 99-117, Tomasz Gwozdz, Karel Dorey
Chapter 7 - The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research, Pages 119-133, Jefte M. Drijvers, Imad M. Awan, Cory A. Perugino, Ian M. Rosenberg, Shiv Pillai
Chapter 8 - Immunofluorescence, Pages 135-150, Sonali Joshi, Dihua Yu
Chapter 9 - Cell Culture: Growing Cells as Model Systems In Vitro, Pages 151-172, Charis-P. Segeritz, Ludovic Vallier
Chapter 10 - Flow Cytometry, Pages 173-189, Eleni Chantzoura, Keisuke Kaji
Chapter 11 - Transfection, Pages 191-209, Semira Sheikh, Amanda S. Coutts, Nicholas B. La Thangue
Chapter 12 - In Vivo Animal Modeling: Drosophila, Pages 211-234, Michael F. Wangler, Hugo J. Bellen
Chapter 13 - Zebrafish as a Research Organism: Danio rerio in Biomedical Research, Pages 235-261, John Collin, Paul Martin
Chapter 14 - Xenopus as a Model Organism for Biomedical Research, Pages 263-290, Shoko Ishibashi, Francesca Y.L. Saldanha, Enrique Amaya
Chapter 15 - Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research, Pages 291-312, Laurens J. Lambert, Mandar D. Muzumdar, William M. Rideout III, Tyler Jacks
Appendix A - Legal Framework on the Scientific Use of Animals in Research, Pages 313-326, David Pettitt, Adam Pettitt, MacKenna Roberts, James Smith, David Brindley
Appendix B - Regulatory Frameworks for Stem Cell Research, Pages 327-338, Eleanor Jane Budge, Morteza Jalali
Appendix C - Using Multiple Experimental Methods to Address Basic Science Research Questions, Pages 339-343, Justyna Zaborowska, Morteza Jalali
Index, Pages 345-355