Bacteriophages: Methods and Protocols, Volume IV

Front Matter ....Pages i-xi
 PhageFISH for Monitoring Phage Infections at Single Cell Level  (Jimena Barrero-Canosa, Cristina Moraru)....Pages 1-26
 Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria  (Mariana Piuri, Graham F. Hatfull)....Pages 27-36
 Engineering Bacteriophage-Based Biosensors  (Daniel Brownell, John King, Brian Caliando, Lada Sycheva, Michael Koeris)....Pages 37-50
 Introduction of Phage Genome into Escherichia coli by Electroporation  (Nika Janež, Saša Haberl Meglič, Karel Flisar, Damijan Miklavčič, Matjaž Peterka)....Pages 51-56
 Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1  (Charles R. Stewart)....Pages 57-67
 Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA  (Laura J. Marinelli, Mariana Piuri, Graham F. Hatfull)....Pages 69-80
 Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography  (Krystyna Dąbrowska)....Pages 81-87
 Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes  (Hany Anany, Luba Y. Brovko, Denis Arutyunov, Nilufar Poshtiban, Amit Singh, Upasana Singh et al.)....Pages 89-105
 Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis  (Lorena Rodríguez-Rubio, David M. Donovan, Beatriz Martínez, Ana Rodríguez, Pilar García)....Pages 107-115
 Analyzing Phage–Host Protein–Protein Interactions Using Strep-tag® II Purifications  (Jeroen De Smet, Hanne Hendrix, An Van den Bossche)....Pages 117-136
 Techniques to Assess Phage–Biofilm Interaction  (Diana Vilas Boas, Carina Almeida, Nuno Azevedo, Sanna Sillankorva, Joana Azeredo)....Pages 137-146
 Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages  (Hanne Hendrix, Ines Staes, Abram Aertsen, Jeroen Wagemans)....Pages 147-162
 Use of Greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for Analysis of Bacterial Pathogenesis  (Fatima Kamal, Danielle L. Peters, Jaclyn G. McCutcheon, Gary B. Dunphy, Jonathan J. Dennis)....Pages 163-171
 Use of a Silkworm Larva Model in Phage Therapy Experiments  (Jumpei Uchiyama, Iyo Takemura-Uchiyama, Shigenobu Matsuzaki)....Pages 173-181
 Exploitation of Drosophila Infection Models to Evaluate Antibacterial Efficacy of Phages  (Hye-Jeong Jang, Hee-Won Bae, You-Hee Cho)....Pages 183-190
 Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems  (Fatima Kamal, Alina Radziwon, Carly M. Davis, Jonathan J. Dennis)....Pages 191-198
 Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy  (Angélina Trotereau, Catherine Schouler)....Pages 199-205
 Quantitating Phage Efficacy in Ready-To-Eat Meats  (Andrew Chibeu, Sampathkumar Balamurugan)....Pages 207-214
Back Matter ....Pages 215-216