Atlas of cilia bioengineering and biocomputing

Front Cover
Half Title Page
RIVER PUBLISHERS SERIES IN BIOMEDICALENGINEERING
Title Page
Copyright Page
Contents
Editor Foreword
List of Contributors
List of Figures
List of Tables
List of Abbreviations
PART I : Biology
	Chapter 1 - Biological Preliminaries for Cilia Study
		1.1 Introduction
		1.2 Primary Cilia
		1.3 Motile Cilia
			1.3.1 Structure
			1.3.2 The Ciliary Beat
		References
	Chapter 2 - Genetic Engineering of Ciliated Cells
		2.1 Introduction
		2.2 Cell Selection and Growth
			2.2.1 Cells with Primary Cilia
			2.2.2 Cells with Motile Cilia
		2.3 Transfection of Cells
		2.4 RNA Interference
		2.5 Fluorescent Protein Labeling
		2.6 Optogenetics
		2.7 Proximity-Dependent Biotinylation (BioID)
		2.8 Tandem Affinity Purification (TAP)
		2.9 Genome Editing Using TALENs, Zn Finger Nucleases, CRISPR/Cas9, and Base Editing
		Acknowledgments
		References
	Chapter 3 - Programming Ciliary Object Manipulation
		3.1 Introduction
		3.2 Sorting by Paramecium Cilia
		3.3 Reprogramming Paramecium Cilia
		Reference
PART II : Engineering
	Chapter 4 - Robotic Cilia for Autonomous Parallel Distributed Sorting Platforms
		4.1 Introduction
		4.2 Engineering Sorting Ciliary Platforms
		References
	Chapter 5 - Artificial Pneumatic Cilia
		5.1 Introduction
		5.2 Base Actuated Pneumatic Cilia
		5.3 Body Actuated Pneumatic Cilia
		5.4 Conclusion
		Acknowledgments
		References
	Chapter 6 - Blinking-Vortex Inspired Mixing with Cilia
		6.1 Cilia Actuation Scheme
		6.2 Experiments and Simulations
		6.3 Results
		References
	Chapter 7 - Magnetic Thin-film Cilia for Microfluidic Applications
		7.1 Introduction
		7.2 Fabrication of Magnetic Cilia
		7.3 Fluid Transport by An Array of Beating Magnetic Cilia
		7.4 Kinematics of Magnetic Cilia
			7.4.1 Counter-Clockwise Rotation of Magnetic Field
			7.4.2 Clockwise Rotation of Magnetic Field
		7.5 Metachronal Motion
		7.6 Microfluidic Applications of Artificial Cilia
			7.6.1 Microfluidic Mixing
			7.6.2 Bacteria Capture
		7.7 Summary
		Acknowledgments
		References
	Chapter 8 - Artificial Cilia: Fabrication, Actuation, and Flow Generation
		8.1 Introduction
		References
PART III : Theoretical and Computer Modelling
	Chapter 9 - Numerical Simulations of Fluid Transport by Magnetically Actuated Artificial Cilia
		9.1 Introduction
		References
	Chapter 10 - Modeling Gel-based Artificial Cilia
		10.1 Introduction
		10.2 Designing Chemo-responsive Cilial Systems
			10.2.1 Methodology: Gel Lattice Spring Model
			10.2.2 Self-oscillating Cilia
		10.3 Photo-responsive Gel-Fiber Systems
		10.4 Capturing Hydrodynamic Interactions in Complex Fluids
			10.4.1 Methodology
			10.4.2 Utilizing Oscillating Rods to “Catch and Release” Targeted Nanoparticles
		10.5 Artificial Cilia Based on Biological Aptamers
			10.5.1 Methodology
			10.5.2 Catch and Release with Aptamer-mimicking Cilia
		10.6 Conclusions
		References
Index
About the Editors
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