Assessing Magnetic Fluid Hyperthermia: Magnetic Relaxation Simulation, Modeling of Nanoparticle Uptake inside Pancreatic Tumor Cells and in vitro Efficacy

Introduction......Page 17
Theory of Magnetism......Page 21
Physics of Magnetic Particle Relaxation......Page 35
Physics of Magnetic Particle Imaging......Page 39
Physics of Magnetic Particle Heating......Page 44
Magnetic Fluid Hyperthermia in Tumor Therapy......Page 47
Hydrogels and Rubber Elasticity Theory......Page 54
State of Research......Page 59
Implementation......Page 60
Results and Comparison to Theories......Page 67
Summary and Outlook......Page 76
Magnetic Particle Systems......Page 79
X-Ray Diffractometry......Page 83
Transmission Electron Microscopy......Page 87
Dynamic Light Scattering......Page 91
Iron Concentration Measurements......Page 95
Superconducting Quantum Interference Device Magnetometry......Page 98
Vibrating Sample Magnetometry......Page 107
Particle Properties Summary......Page 110
Magnetic Particle Spectroscopy......Page 113
Magnetic Fluid Hyperthermia......Page 116
Optimizing Particle Heating by Combining Experiment and Simulation......Page 127
Cell Culture Protocol......Page 135
Morphology of Magnetic Particles inside Cells......Page 137
Quantification of Magnetic Particle Uptake Kinetics......Page 146
Magnetic Properties of Internalized Particles......Page 158
Concluding Remarks......Page 161
Magnetic Particle Heating in Model Systems......Page 165
Characterization of Ferrohydrogels......Page 166
Effect of Particle Immobilization on Magnetic Particles Heating......Page 176
Control and Characterization of Agglomerated Magnetic Particles......Page 183
Effect of Particle Agglomeration on Magnetic Particle Heating......Page 195
Magnetic Fluid Hyperthermia Efficacy......Page 199
Experimental Procedure......Page 200
Magnetic Fluid Hyperthermia Effects on Living Cells......Page 203
Concluding Remarks on Intracellular Magnetic Fluid Hyperthermia Applicability......Page 213
Conclusion and Future Directions......Page 217
Appendix......Page 221
Supplementary Information on Chapter 2......Page 222
Supplementary Information on Chapter 4......Page 223
Supplementary Information on Chapter 5......Page 232
Supplementary Information on Chapter 6......Page 238
Supplementary Information on Chapter 7......Page 242
Supplementary Information on Chapter 8......Page 249
Bibliography......Page 257
List of Publications......Page 287
Acknowledgment......Page 289