Aspartic Proteinases: Retroviral and Cellular Enzymes

Front Matter....Pages i-xvii
The Aspartic Proteinases....Pages 1-13
The Regulation of Sequential Processing of HIV-1 Gag by the Viral Protease....Pages 15-25
A Cellular Anti-Apoptosis Protein is Cleaved by the HIV-1 Protease....Pages 27-29
Engineering Catalytically Defective Forms of HIV Protease to Modulate Its Activity....Pages 31-39
The Aspartic Proteinase from Equine Infectious Anaemia Virus....Pages 41-45
The Effect of Substrates on the Kinetics and the in Vivo Threshold Activity of Mutant HIV-1 Proteases....Pages 47-51
A Comparison of gag—pol Precursor Cleavage in Naturally Arising HIV Variants....Pages 53-57
X-Ray Crystallographic Studies of the Structure-Function Relationships of HIV-1 Protease....Pages 59-63
Optimization of a Macromolecular Inhibitor of HIV-1 Protease....Pages 65-70
Back Matter....Pages 71-74
 In Vitro Selection and Characterization of VX-478 Resistant HIV-1 Variants....Pages 75-83
Sensitivity to Inhibition and Catalytic Efficiency of HIV Proteinase Mutants....Pages 85-89
Computer Assisted Evaluation of Inhibitor Sensitivity to HIV-1 PR Mutants....Pages 91-97
Investigation of an Allosteric Site of HIV-1 Proteinase Involved in Inhibition by Cu 2+ ....Pages 99-103
Analysis of Autoprocessing of Mason-Pfizer Monkey Virus Proteinase in Vitro ....Pages 105-108
Subsequent Selfprocessing of Bovine Leukemia Virus Proteinase in Vitro ....Pages 109-113
Mechanism of Action of Aspartic Proteases....Pages 115-121
Theory and Method of a Priori Computation of Catalytic Acts of Aspartic and Serine Proteinases....Pages 123-126
Back Matter....Pages 127-132
Comparison of The Specificity of the Aspartic Proteinases Towards Internally Consistent Sets of Oligopeptide Substrates....Pages 133-138
Expression of Chimeric Human Aspartic Proteinases....Pages 139-146
Cathepsin E....Pages 147-152
Back Matter....Pages 223-228
Biosynthesis and Intracellular Targeting of the Lysosomal Aspartic Proteinase Cathepsin D....Pages 153-162
An Aspartic Proteinase Expressed in the Equine Placenta....Pages 163-167
Protein Engineering Aspartic Proteinases....Pages 169-177
Post X-Ray Crystallographic Studies of Chymosin Specificity....Pages 179-184
Epitope Mapping of Recombinant Human Procathepsin D....Pages 185-189
Construction of Chimeric Enzymes to Probe Subsite Contributions to Catalytic Specificity....Pages 191-194
Expression of Multiple Genes for Pregnancy-Associated Glycoproteins in the Sheep Placenta....Pages 195-200
Mass Spectrometry as a Tool for Studying the Action of Human Aspartic Proteases on Peptides and Proteins....Pages 201-206
Skin Surface Proteolytic Activity....Pages 207-212
Cathepsin E in the Central Nervous System....Pages 213-217
Detailed Analysis of Human Cathepsin E Prime Region Specificity....Pages 219-222
Back Matter....Pages 223-228
Proteolytic and Non-Proteolytic Activation of Prorenin....Pages 229-238
The Roles of the Basic Residues in the Prosegment of Aspergillopepsinogen I....Pages 239-244
Activation Mechanism of Pepsinogen as Compared to the Processing of HIV Protease gag-pol Precursor Protein....Pages 245-252
Cardosinogen A....Pages 253-258
Crystal Structure of Human Pepsinogen A....Pages 259-263
Crystallographic Studies of an Activation Intermediate of Human Gastricsin....Pages 265-270
Back Matter....Pages 271-273
 Aspergillus niger Acid Proteinase A....Pages 275-282
Crystal Structure of The Rhizomucor miehei Aspartic Proteinase....Pages 283-292
 Rhizomucor miehei Aspartic Proteinases Having Improved Properties....Pages 293-296
Back Matter....Pages 361-362
Structure of Secreted Aspartic Proteinases from Candida ....Pages 297-313
Yapsin1....Pages 315-319
Bacterial Aspartic Proteinases as Novel Antibiotic Targets....Pages 321-324
Structural Thermodynamic Study of the Binding of Renin Inhibitors to Endothiapepsin....Pages 325-328
Development and Testing of Inhibitors of Candida Aspartic Proteinases....Pages 329-333
Primary Substrate Specificities of Secreted Aspartic Proteases of Candida albicans ....Pages 335-338
Functional Aspects of Secreted Candida Proteinases....Pages 339-344
Substrate Specificity of Non-Pepsin-Type Acid Proteinase, Aspergillus niger Proteinase A....Pages 345-348
Pepstatin-Insensitive Carboxyl Proteinases from Prokaryotes....Pages 349-353
Overcoming the Unfavourable Entropic Contribution of Ligand Binding with a Macrocyclic Inhibitor Bound to Penicillopepsin....Pages 355-359
Back Matter....Pages 361-362
Structural Analysis of Plasmepsin II....Pages 363-373
Screening Aspartyl Proteases with Combinatorial Libraries....Pages 375-380
Thermodynamics and Proton Uptake for Pepstatin Binding to Retroviral and Eukaryotic Aspartic Proteases....Pages 381-386
Expression, Purification, and Characterization of the Recombinant Pepsin Inhibitor from Ascaris suum ....Pages 387-389
X-Ray Crystallographic Studies of the Complex Between Porcine Pepsin and the Aspartic Proteinase Inhibitor PI-3 from the Nematode Ascaris suum ....Pages 391-395
Studies on Plasmepsins I and II from the Malarial Parasite Plasmodium falciparum and their Exploitation as Drug Targets....Pages 397-406
Plasmepsins I and II from the Malarial Parasite Plasmodium falciparum ....Pages 407-411
Back Matter....Pages 413-421
Structural and Functional Aspects of Cardosins....Pages 423-433
Molecular Cloning of Aspartic Proteinases from Flowers of Cynara cardunculus SUBSP. flavescens CV. Cardoon and Centaurea calcitrapa ....Pages 435-439
Aspartic Proteinases and Inhibitors in Plant Pathogenesis....Pages 441-444
Back Matter....Pages 481-483
Crystallisation, Structure Solution, and Initial Refinement of Plant Cardosin-A....Pages 445-452
Acid Proteinase From Nepenthes distillatoria (Badura)....Pages 453-458
A Comparative Study on the Aspartic Proteinases from Different Species of Cynara....Pages 459-463
An Aspartic Proteinase from Flowers of Centaurea calcitrapa ....Pages 465-472
Substrate Specificity and Molecular Modelling of Aspartic Proteinases (Cyprosins) from Flowers of Cynara cardunculus SUBSP. flavescens CV. Cardoon....Pages 473-479
Back Matter....Pages 481-483
Back Matter....Pages 485-499