Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics

Content: 
Contributors to volume 328
Pages ix-xii

Preface
Pages xiii-xiv
Jeremy Thorner, Scott D. Emr, John N. Abelson

Methods in enzymology
Pages xv-xxxiv

[1] High-throughput screening for protein-protein interactions using two-hybrid assay Original Research Article
Pages 3-14
Gerard Cagney, Peter Uetz, Stanley Fields

[2] LexA-based two-hybrid systems Original Research Article
Pages 14-26
Sarah J. Fashena, Ilya G. Serebriiskii, Erica A. Golemis

[3] Interaction mating methods in two-hybrid systems Original Research Article
Pages 26-46
Mikhail G. Kolonin, Jinhui Zhong, Russell L. Finley Jr.

[4] Analysis and identification of protein-protein interactions using protein recruitment systems Original Research Article
Pages 47-59
Ami Aronheim, Michael Karin

[5] A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli Original Research Article
Pages 59-73
Gouzel Karimova, Agnes Ullmann, Daniel Ladant

[6] A green fluorescent protein-based reverse two-hybrid system: Application to the characterization of large numbers of potential protein-protein interactions Original Research Article
Pages 74-88,IN1
Hideki Endoh, Albertha J.M. Walhout, Marc Vidal

[7] Yeast three-hybrid system for detecting ligand-receptor interactions Original Research Article
Pages 89-103
Eric C. Griffith, Edward J. Licitra, Jun O. Liu

[8] Use of a yeast three-hybrid system to clone bridging proteins Original Research Article
Pages 103-110
Jie Zhang

[9] The yeast tribrid system: cDNA expression cloning of protein interactions dependent on posttranslational modifications Original Research Article
Pages 111-127
Jarema P. Kochan, Christoph Volpers, Mark A. Osborne

[10] The yeast split-hybrid system Original Research Article
Pages 128-137
Anthony J. Demaggio, Phyllis Goodman, Hsiu-Ming Shih, Richard H. Goodman, Merl F. Hoekstra

[11] Mapping protein-protein interaction domains using ordered fragment ladder far-Western analysis of hexahistidine-tagged fusion proteins Original Research Article
Pages 141-157
Richard R. Burgess, Terrance M. Arthur, Bradley C. Pietz

[12] Mapping specificity determinants for protein-protein association using protein fusions and random peptide libraries Original Research Article
Pages 157-170
Michael B. Yaffe, Lewis C. Cantley

[13] Selection of genetic agents from random peptide aptamer expression libraries
Pages 171-208
C. Ronald Geyer, Roger Brent

[14] Detection of protein-protein interactions by protein fragment complementation strategies
Pages 208-230
Stephen W. Michnick, Ingrid Remy, François-X. Campbell-Valois, Alexis Vallée-Bélisle, Joelle N. Pelletier

[15] Monitoring protein-protein interactions in live mammalian cells by,8-galactosidase complementation Original Research Article
Pages 231-251,IN3-IN4
Fabio M.V. Rossi, Bruce T. Blakely, Carol A. Charlton, Helen M. Blau

[16] Green fluorescent protein chimeras to probe protein-protein interactions
Pages 251-261
Sang-Hyun Park, Ronald T. Raines

[17] Protein fusions to coiled-coil domains
Pages 261-282
Kristian M. MГјller, Katja M. Arndt, Tom Alber

[18] Molecular applications of fusions to leucine zippers
Pages 282-296
Jennifer D. Rieker, James C. Hu

[19] Using the yeast three-hybrid system to detect and analyze RNA-protein interactions Original Research Article
Pages 297-321
Brian Kraemer, Beilin Zhang, Dhruba Sengupta, Stanley Fields, Marvin Wickens

[20] Principles and applications of a tat-based assay for analyzing specific RNA-protein interactions in mammalian cells Original Research Article
Pages 322-329
Bryan R. Cullen

[21] Phage display for selection of novel binding peptides Original Research Article
Pages 333-363,IN5
Sachdev S. Sidhu, Henry B. Lowman, Brian C. Cunningham, James A. Wells

[22] Selectively infective phage technology Original Research Article
Pages 364-388
Katja M. Arndt, Sabine Jung, Claus Krebber, Andreas PlГјckthun

[23] Use of phage display and transition-state analogs to select enzyme variants with altered catalytic properties: Glutathione transferase as an example Original Research Article
Pages 389-404
Mikael Widersten, Lars O. Hansson, Lisa Tronstad, Bengt Mannervik

[24] Selecting and evolving functional proteins in vitro by ribosome display Original Research Article
Pages 404-430
Jozef Hanes, Lutz Jermutus, Andreas PlГјckthun

[25] Yeast surface display for directed evolution of protein expression, affinity, and stability Original Research Article
Pages 430-444
Eric T. Boder, K. Dane Wittrup

[26] Methods for in vitro DNA recombination and random chimeragenesis Original Research Article
Pages 447-456
Alexander A. Volkov, Frances H. Arnold

[27] Random chimeragenesis by heteroduplex recombination Original Research Article
Pages 456-463
Alexander A. Volkov, Zhixin Shao, Frances H. Arnold

[28] Use of chimeras generated by DNA shuffling: Probing structure-function relationships among glutathione transferases Original Research Article
Pages 463-477
Lars O. Hansson, Bengt Mannervik

[29] Protein engineering by expressed protein ligation Original Research Article
Pages 478-496
Ulrich K. Blaschke, Jonathan Silberstein, Tom W. Muir

[30] A systematic and general proteolytic method for defining structural and functional domains of proteins Original Research Article
Pages 499-514
Jannette Carey

[31] High-throughput expression of fusion proteins Original Research Article
Pages 515-529
Marc Nasoff, Mark Bergseid, James P. Hoeffler, John A. Heyman

[32] Rapid construction of recombinant DNA by the univector plasmid-fusion system Original Research Article
Pages 530-549
Qinghua Liu, Mamie Z. Li, Dou Liu, Stephen J. Elledge

[33] High-throughput methods for the large-scale analysis of gene function by transposon tagging Original Research Article
Pages 550-574
Anuj Kumar, Shelley Ann des Etages, Paulo S.R. Coelho, G. Shirleen Roeder, Michael Snyder

[34] GATEWAY recombinational cloning: Application to the cloning of large numbers of open reading frames or ORFeomes Original Research Article
Pages 575-592,IN7
Albertha J.M. Walhout, Gary F. Temple, Michael A. Brasch, James L. Hartley, Monique A. Lorson, Sander van den Heuvel, Marc Vidal

[35] Gene trapping methods for the identification and functional analysis of cell surface proteins in mice Original Research Article
Pages 592-615
William C. Skarnes

Author index
Pages 617-648

Subject index
Pages 649-666