Applications of Chimeric Genes and Hybrid Proteins Part A: Gene Expression and Protein Purification

Content: 
Contributors to volume 326
Pages ix-xi

Preface
Pages xiii-xiv
Jeremy Thorner, Scott D. Emr, John N. Abelson

Volumes in series
Pages xv-xxxiii

[1] The all purpose gene fusion Original Research Article
Pages 3-7
Jon Beckwith

[2] A practical guide to the construction and use of lac fusions in Escherichia coli Original Research Article
Pages 11-35
Nicholas J. Hand, Thomas J. Silhavy

[3] Tagging exported proteins using Escherichia coli alkaline phosphatase gene fusions Original Research Article
Pages 35-47
Colin Manoil

[4] Applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells Original Research Article
Pages 47-73
Raphael H. Valdivia, Lalita Ramakrishnan

[5] IVET and RIVET: Use of gene fusions to identify bacterial virulence factors specifically induced in host tissues Original Research Article
Pages 73-96
James M. Slauch, Andrew Camilli

[6] Identification of exported bacterial proteins via gene fusions to Yersinia pseudotuberculosis invasin Original Research Article
Pages 97-104
Micah J. Worley, Fred Heffron

[7] Use of imidazoleglycerolphosphate dehydratase (His3) as a biological reporter in yeast Original Research Article
Pages 107-119
Joe Horecka, George F. Sprague Jr.

[8] Use of fusions to human thymidine kinase as reporters of gene expression and protein stability in Saccharomyces cerevisiae Original Research Article
Pages 120-133
Sofie R. Salama

[9] Use of fusions to thymidine kinase Original Research Article
Pages 134-146
Christiaan Karreman

[10] In Vivo analysis of lacZ fusion genes in transgenic Drosophila melanogaster Original Research Article
Pages 146-159
Stephen Small

[11] Utility of the secreted placental alkaline phosphatase reporter enzyme Original Research Article
Pages 159-164
Bryan R. Cullen

[12] Fusions to imidazopyrazinone-type luciferases and aequorin as reporters Original Research Article
Pages 165-174
Satoshi Inouye

[13] Novel methods for chemiluminescent detection of reporter enzymes Original Research Article
Pages 175-202
Corinne E.M. Olesen, Yu-Xin Yan, Betty Liu, Dina Martin, Brian D\'Eon, Ray Judware, Chris Martin, John C. Voyta, Irena Bronstein

[14] Fusions to chloramphenicol acetyltransferase as a reporter Original Research Article
Pages 202-221
Clayton Bullock, Cornelia Gorman

[15] Fusions to ОІ-lactamase as a reporter for gene expression in live mammalian cells Original Research Article
Pages 221-241
Gregor Zlokarnik

[16] Purification of proteins using polyhistidine affinity tags Original Research Article
Pages 245-254
Joshua A. Bornhorst, Joseph J. Falke

[17] Generating fusions to glutathione S-transferase for protein studies Original Research Article
Pages 254-270
Donald B. Smith

[18] Use of the Strep- tag and streptavidin for detection and purification of recombinant proteins Original Research Article
Pages 271-304
Arne Skerra, Thomas G.M. Schmidt

[19] Streptavidin-containing chimeric proteins: design and production Original Research Article
Pages 305-311
Takeshi Sano, Charles R. Cantor

[20] Fusions to maltose-binding protein: Control of folding and solubility in protein purification Original Research Article
Pages 312-321
Deepali Sachdev, John M. Chirgwin

[21] Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli Original Research Article
Pages 322-340
Edward R. LaVallie, Zhijian Lu, Elizabeth A. Diblasio-Smith, Lisa A. Collins-Racie, John M. McCoy

[22] Affinity purification of recombinant proteins fused to calmodulin or to calmodulin-binding peptides Original Research Article
Pages 340-362
Peter Vaillancourt, Chao-Feng Zheng, Danny Q. Hoang, Lisa Breister

[23] The SВ·tag fusion system for protein purification Original Research Article
Pages 362-376
Ronald T. Raines, Mark McCormick, Thomas R. Van Oosbree, Robert C. Mierendorf

[24] Fusions to self-splicing inteins for protein purification Original Research Article
Pages 376-418
Ming-Qun Xu, Henry Paulus, Shaorong Chong

[25] Fusion proteins containing cellulose-binding domains Original Research Article
Pages 418-429
Jae-Seon Park, Hae-Sun Shin, Roy H. Doi

[26] Biotinylation of proteins in vivo and in vitro using small peptide tags Original Research Article
Pages 430-440
Millard G. Cull, Peter J. Schatz

[27] Biotinylation of proteins in vivo: A useful posttranslational modification for protein analysis Original Research Article
Pages 440-458
John E. Cronan Jr., Kelynne E. Reed

[28] Methods for generating multivalent and bispecific antibody fragments Original Research Article
Pages 461-479
Ian Tomlinson, Philipp Holliger

[29] Design and use of phage display libraries for the selection of antibodies and enzymes Original Research Article
Pages 480-505
Francesca Viti, Fredrik Nilsson, Salvatore Demartis, Adrian Huber, Dario Neri

[30] Use of an Lpp-OmpA fusion vehicle for bacterial surface display Original Research Article
Pages 506-516
Charles F. Earhart

[31] Identification of bacterial class I accessible proteins by disseminated insertion of class I epitopes Original Research Article
Pages 516-527
Dolph Ellefson, Adrianus W.M. van der Velden, David Parker, Fred Heffron

[32] Bordetella pertussis adenylate cyclase toxin: A vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells Original Research Article
Pages 527-542
Pierre Guermonprez, Catherine Fayolle, Gouzel Karimova, Agnes Ullmann, Claude Leclerc, Daniel Ladant

[33] Use of anthrax toxin fusions to stimulate immune responses Original Research Article
Pages 542-551
Christopher C. Zarozinski, R. John Collier, Michael N. Starnbach

[34] Use of fusions to viral coat proteins as antigenic carriers for vaccine development Original Research Article
Pages 551-569
Peter G. Stockley, Robert A. Mastico

Author index
Pages 571-600

Subject index
Pages 601-617