Animal Cell Technology: From Vaccines to Genetic Medicine

Front Matter....Pages i-xviii
Front Matter....Pages 1-1
Molecular Biology of Prion Diseases....Pages 3-6
Applications of Animal Cell Technology to Drug Discovery and Pharmacology....Pages 7-14
Biogenesis of Secretory Proteins and Cell Surface Receptors in Mammalian Cells: Posttranslational Modifications and Protein Folding within the Rough Endoplasmic Reticulum....Pages 15-26
Front Matter....Pages 27-27
Polymer Encapsulated Xenogeneic Cell Lines: A Novel Approach for Gene Therapy....Pages 29-31
Gene Therapy and Genetic Vaccination: Two Hallmarks of Genetic Medicine....Pages 33-35
PCR Detection of Murine Retroviruses and Characterization of a New Xenotropic Retrovirus from Myeloma Cells....Pages 37-42
Toward a DNA Vaccine Against Lyssa Viruses....Pages 43-46
Principles of a Scaleable Transient Transfection and Expression Technology for Mammalian Cells....Pages 47-50
Neurological Therapy — Adenovirus Mediated Gene Therapy in Cells of the Central Nervous System....Pages 51-56
Bovine Polyoma Virus Detection by Routine PCR Screening....Pages 57-62
Front Matter....Pages 63-63
Mammalian Cells in High Throughput Screening....Pages 65-71
Metabolism and Toxicological Studies in Immortalised Rat Hepatocyte Cell Lines....Pages 73-78
Cell Based Assays for Drug Screening....Pages 79-84
Development of Drug Metabolism Simulator Using Three-Dimensional Culture of Hepatocytes of Rats and Dogs....Pages 85-90
Immobilization of Primary Brain Cells in Porous Microcarriers for on Line NMR Spectroscopy....Pages 91-98
Application of primary hepatocyte sandwich cultures for predictive studies of drug metabolism and precarcinogen activation....Pages 99-102
Increased cytotoxic sensibility of cultured fathead minnow fish cells by simultaneous treatment with sodium dodecyl sulfate and buthionine sulfoximine....Pages 103-107
Immortalization and Characterization of Porcine Brain Microcapillary Endothelial Cells....Pages 109-113
Three-dimensional Cell Culture Systems Stabilise the Differentiation of Hepatocyte Cell Lines....Pages 115-119
In-Vitro Toxicological Effects of Pluronic F-68....Pages 121-126
Front Matter....Pages 63-63
Genotoxicity of bacterial ribonuclease in mammalian cells after its application in vitro and in vivo ....Pages 127-132
Use of Cell-Based Assays for in Vitro Studies of Drugs....Pages 133-138
Evaluation of Cytotoxicity with Fish Cell Lines: A Case Study for α-Oligothiophenes (1-5 Rings)....Pages 139-143
The Effect of Cell Culture System on the Phenotypic Stability in Primary Hepatocyte Cells....Pages 145-149
Front Matter....Pages 151-151
New Emerging Viruses: Potential Risk of Contamination of Biological Products....Pages 153-157
Development and Production of New Live Measles Vaccine Based on Human Embryo Lung Diploid Cell Culture L-68....Pages 159-164
Development of influenza subunit vaccine produced using mammalian cell culture technology....Pages 165-167
Monitoring and control of large — scale vaccine production via dynamic oxygen uptake rate measurement....Pages 169-174
Reactor Development for the Hepatitis A Vaccine VAQTA™....Pages 175-183
Production of Live Attenuated Varicella-Zoster Virus in Human Embryonic Lung Cells Using Microcarrier....Pages 185-189
Production of Lyssa Virus Proteins in the Baculovirus Expression System and their Use as Potential Vaccine....Pages 191-195
Development of Production Technology of Live Measles Vaccine for Peroral Administration....Pages 197-202
Front Matter....Pages 203-203
On-Line Monitoring for Consistent and Optimal Production of Biologicals from Mammalian Cell Cultures....Pages 205-214
On the Use of Electric Fields to Prevent Membrane Fouling of Process Integrated Cross Flow Filtration at Hybridoma Cell Cultures....Pages 215-220
Simplified screening of monoclonal antibodies for immunoaffinity chromatography — A case study on anti-hIL-2-antibodies....Pages 221-226
Integrative Antibody Purification from whole Broth by Fluidized Bed Adsorption....Pages 227-233
Large-Scale Production of Humanized Monoclonal Antibody Expressed in a GS-NSO Cell Line....Pages 235-241
2D PAGE examination of the proteins extracted from high density perfused HeLa cell cultures controlled by an on-line capacitance-based biomass probe....Pages 243-249
Experimental and theoretical analysis of cell separations by ultrasonic forces....Pages 251-256
Factors Affecting the Production and Quality of IgA Antibodies Active Against V. cholerae ....Pages 257-264
Front Matter....Pages 203-203
Ceramic Hydroxyapatite: A New Tool for Separation and Analysis of IGA Monoclonal Antibodies....Pages 265-269
“Bionebulisation”: an alternative to Dounce homogenization for the preparation of mammalian cells nuclei in bulk....Pages 271-275
Epitope Determination for Antibodies Raised Against Recombinant Human Interferon-Gamma....Pages 277-282
A New Spinfilter Design....Pages 283-288
On-line Biomass Monitoring of Immobilized Hybridoma Cells by Dielectrical Measurements....Pages 289-294
Evaluation of the Proteolytic Activity Present in Cho Cell Culture Supernatants....Pages 295-300
CeDEX: Automated Cell Density Determination....Pages 301-305
Design and Performance of a Perfusion Based Packed Bed Bioreactor for the Production of Secreted Protein....Pages 307-312
Low- and High-Producing Animal Cell Cultures in Perfused, Packed Beds of Non-Woven Fabric....Pages 313-318
Temperature a Factor Influencing Cell Behaviour....Pages 319-323
Direct Capture of Monoclonal Antibodies using High Capacity Membrane Ion Exchangers in Pilot Scale....Pages 325-329
Influence of Mass Culture Operating Conditions on the Apoptosis of OKT3 Hybridoma Cells....Pages 331-336
Increased Production of Recombinant hIGFBP-1 in Self-Fused Chinese Hamster Ovary (CHO) Cells....Pages 337-342
New Process for Cell Detachment: Use of Heparin....Pages 343-348
Development of a High Sensitivity Retrovirus Specific Reverse Transcriptase Assay....Pages 349-354
Kinetics of Glucose Metabolism and Utilization of Lactate in Mammalian Cell Cultures....Pages 355-360
”Nutrient-Split” Feeding Strategy for Dialysis Cultures of Immobilized Hybridoma and Transfectoma Cells....Pages 361-366
Immobilization Can Improve the Culture Stability of Non-Anchorage-Dependent Animal Cells in Serum-Free Media....Pages 367-371
Influence of Pluronic F-68 on the Ultrafiltration of Cell Culture Supernatants....Pages 373-378
Dynamic Membrane Filtration in Mammalian Cell Culture Harvest....Pages 379-384
Front Matter....Pages 203-203
Culture of Human Intestinal Cells in Simulated Microgravity....Pages 385-389
Removal of animal cells and cell debris by continuous two-stage centrifugation....Pages 391-397
Large Scale Bubble Bed Aeration for Animal Cell Cultures....Pages 399-404
Contribution to the Understanding of Retention of Animal Cells in Spinfilters....Pages 405-409
Rotating Disk Microfiltration of Cell Culture Broth — Effect of Shear Force and Cell Stability on Filtration Performance....Pages 411-416
Process Control of Antithrombin III Production by Near-Infrared Spectroscopy....Pages 417-421
Selection of an Immobilization Method for a Perfusion Bioreactor with Hybridoma Cells....Pages 423-428
On-Line Monitoring of Glutamine and Ammonium in Mammalian-Cell Cultures....Pages 429-434
Study for the Production of a Recombinant Monoclonal Antibody in Stirred Tank....Pages 435-440
Strategy for Scale Up of Mammalian Cell Culture in the New GMP Manufacturing Facility of the Cuban Biotechnology Industry....Pages 441-446
Process Development for the Large Scale Production of Clinical Grade Human Monoclonal Antibody Using a Cytopilot Fluidized Bed Reactor....Pages 447-450
Scale-Up Optimization Based on the Development of a Perfusion Rate Control for CHO Cells Using a Cytopilot Fluidized Bed Reactor....Pages 451-454
Development of CHO cell serum-free culture process for expression of recombinant viral glycoprotein....Pages 455-459
Cell Productivity is Overestimated by the Crystal Violet Nuclei Counting Technique....Pages 461-466
Residual DNA from Hybridoma Cultures: Decorrence of Apoptosis?....Pages 467-472
Front Matter....Pages 473-473
Expression of human α2, 6-Sialyltransferase in BHK-21A cells increases the sialylation of coexpressed human erythropoietin: NeuAc-transfer onto GalNAc(βl-4)GlcNAc-R motives....Pages 475-480
Construction of novel BHK-21 cell lines coexpressing Golgi resident or soluble forms of human α2,6-sialyltransferase and α1,3/4-fucosyltransferases together with secretory glycoproteins....Pages 481-487
Metabolic Engineering of N-Linked Glycoform Synthesis Systems in Chinese Hamster Ovary (CHO) Cells....Pages 489-494
Extracellular Propeptide Processing of Recombinant Human Factor Ix by a Secreted Form of the Endoprotease, Pace....Pages 495-501
Glycosylation patterns of recombinant therapeutic proteins produced in two mammalian cell lines....Pages 503-509
Front Matter....Pages 473-473
Construction of Recombinant Cell Lines with Defined Properties Using FLP Recombinase Driven Gene Replacement....Pages 511-517
High Level Expression of Foreign Gene in Chinese Hamster Ovary Cells Using Modified Tetracycline-Regulated System....Pages 519-523
Expression of Triabin, a Novel Highly Potent Inhibitor of Thrombin, in Sf9 and High Five Insect Cells using a Recombinant Baculovirus....Pages 525-528
Expression Augmenting Sequence Element (EASE) Isolated From Chinese Hamster Ovary Cells....Pages 529-534
Continuous Production of the Extracellular Domain of Recombinant Human Ca ++ Receptor from HEK 293 Cells Using Novel Serum Free Medium....Pages 535-540
Front Matter....Pages 541-541
Bioreactor for a Hybrid Liver Support....Pages 543-555
Factors Affecting Megakaryocytic Expansion in Hematopoietic Cultures for Transplant Therapies....Pages 557-563
The possible role of the tumor suppressor protein p53 in neonatal and adult cardiomyocytes....Pages 565-570
Characterization of Extracellular Matrix in Three-Dimensional Cultures of DU 145 Human Prostate Carcinoma Cells....Pages 571-575
Spheroid Formation of Primary Dog Hepatocytes Using Polyurethane Foam and its Application to Hybrid Artificial Liver....Pages 577-583
The bilaminar membrane (BLM) bioreactor for hepatic tissue engineering with primary porcine hepatocytes....Pages 585-587
Differentiation of Bone Marrow Cells Immobilized on Microcarriers in a Fluidized Bed Reactor....Pages 589-593
The Biochip- a New Cell Factory for the Cell Cultivation in Defined Three-Dimensional Layers....Pages 595-600
Front Matter....Pages 601-601
Animal Cells and Serum as Natural Sources of Chemokines....Pages 603-605
 De novo formation in mice of adipose tissue and muscle fibers at the site of injecting Matrigel and growth factors....Pages 607-612
Overexpression of the apoptosis suppressor gene, bcl-2 , in hybridoma cells prolongs viability and increases productivity in batch culture....Pages 613-620
Deregulated Expression of Cloned Transcription Factor E2F-1 in Chinese Hamster Ovary Cells Shifts Protein Patterns and Activates Growth in Protein-Free Medium....Pages 621-623
The influence of long-chain bases (sphingolipids) on early stages of T lymphocyte activation....Pages 625-632
Metabolic Flux Analysis of Hybridoma Cells Using Mass Balances, 13 CO 2 Mass Spectrometry and NMR Spectrometry....Pages 633-638
Model-Based Control of Animal Cell Cultures....Pages 639-645
Front Matter....Pages 601-601
Alteration in Glycosylation: Controlling the Diversity of Recombinant Glycoproteins....Pages 647-649
Inhibitors of Cell Growth: Accumulation and Concentration....Pages 651-655
Optimisation of the Fatty Acid Composition of a Hybridoma Enhances Mab Production and Perturbs Energy Metabolism....Pages 657-662
Effect of Power Input in Virus Like Particles Production....Pages 663-668
Isolation and Characterization of a CHO Cell Line Expressing rhFSH in Low Protein and Protein-Free Media....Pages 669-674
Unbalanced Media for Hybridoma Cell Culture —an Alternative Reality....Pages 675-680
Viral Contaminants Found in Mouse Cell Lines Used in the Production of Biological Products....Pages 681-686
Analysis of Endogenous Retrovirus Production in a Murine Hybridoma by Flow Cytometry....Pages 687-690
Construction of a Human Chondromodulin-I Expression System in CHO Cells....Pages 691-695
Elucidation of metabolic network of mammalian cell line K562 using 2- 14 C glucose label and stoichiometric modelling....Pages 697-702
Expression of a Protein A-Fucosyltransferase III fusion protein in insect cells....Pages 703-707
Adaptation to Serum Free Media of BHK Cells Producing a recIgG....Pages 709-713
Product Quality of a recIgG Under Different Culture Systems of BHK Cells....Pages 715-720
A Potent Growth Factor Supplement For Cell Culture Purified From Whey....Pages 721-724
Development of carbon balances for continuous animal cell cultures using 14 -C labelled glucose....Pages 725-729
The Mechanical Strength of Mammalian Cells During Mitotic Cell Division....Pages 731-736
Development of Unique Growth Factors for Serum-Free Cell Culture using Fusion Partner Technology....Pages 737-741
A Serum-Free Medium Developed to Support the Growth of Several Fibroblastic Cell Lines....Pages 743-748
Transfection of Human Placental Endothelial Cells with SV40 T-Antigens....Pages 749-753
Characterization of growth and recombinant protein production of the insect cell line BTI-Tn-5Bl-4 (High Five™) in suspension culture....Pages 755-760
Front Matter....Pages 601-601
Controlling the Glycosylation of Recombinant Proteins Expressed in Animal Cells by Genetic and Physiological Engineering....Pages 761-765
Estimation of Metabolic Fluxes Using Material Balances for a CHO Cell Line....Pages 767-772
Physiological and Kinetic Studies on Two Hybridomas Secretingmonoclonalantibodies in Bioreactors....Pages 773-778
Hydrodynamic Control of Aggregate Size in Suspended Cultures of Different Animal cells....Pages 779-783
Analysis of Glucose and Glutamine Metabolism of Hybridoma Cells by Continuous Culture Experiments....Pages 785-789
The Metabolic Profile of a Murine Hybridoma Grown in Serum Free Medium at Various Oxygen Concentrations....Pages 791-796
Alternative Strategies for the Production of an Antibody-Cytokine Fusion Protein from Recombinant BHK Cells....Pages 797-800
Optimization of Human Antibody Production by Xenohybridization of EBV-Transformed Cell Lines....Pages 801-806
Back Matter....Pages 807-816