Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Fifth International Meeting of the Japanese Association for Animal Cell Technology, Omiya, Japan, November 30—December 4, 1992

Front Matter....Pages i-xvii
Search for Edible Plant Constituents of Anti-Tumor Promotion Using an Inhibition Test of Epstein-Barr Virus Activation in Raji Cells....Pages 1-8
The Nature and Effect of Cell-Cell Interactions between Recombinant CHO Cells Producing Human Interferon-Gamma.....Pages 9-18
Characterization of Newly Established Cells Which Provide an Animal Model for Spontaneous Metastasis....Pages 19-24
Bovine Mammary Epithelial Cells: Morphology, Protein Synthesis and Gene Transfection....Pages 25-31
Growth and Differentiation of a Small Intestinal Epithelial Cell Line IEC-6....Pages 33-40
Subunit Assembly of Laminin Variants in Cultured Animal Cells....Pages 41-48
Regulation of Mitochondrial Gene Expression by Interferons....Pages 49-55
Production of the Human Growth Hormone in Serum-Free UC203 Medium....Pages 57-61
Stable Production of Pro-urokinase and its Derivative by Namalwa KJM-1 Cells Adapted to a Serum-free Medium....Pages 63-70
Production and Characterization of Recombinant Soluble form Erythropoietin Receptor....Pages 71-77
A ts-SV40 Based New Mammalian Plasmid Vector and Its Copy Number Control by Temperature Change....Pages 79-84
Selection of Regulatory Mutants of μ Gene Expression from Myeloma Cells Transfected with the Modified μ Gene....Pages 85-91
Process Control of BHK Cells by Nucleotide Pool Measurement....Pages 93-97
Purification and cDNA Cloning of Flatfish Interferon....Pages 99-105
Detection by Fluorescence in Situ Hybridization of the Erythropoietin Gene Introduced into Animal Cells....Pages 107-113
High-Level Expression of Recombinant Bovine ß-Lactoglobulin in Insect Cells....Pages 115-121
Mass Production and Purification of Recombinant Flatfish Interferon....Pages 123-127
Stimulation of Protein Degradation By Tumor Necrosis Factor a in Cultured Muscle Cells....Pages 129-133
From in Vitro Cell Culture to in Vivo Animal Study: Demonstration that Epidermal Growth Factor (EGF) Is a Potent Inhibitor of Adipose Differentiation.....Pages 135-141
Co-Operative Action of Cysteine and Some Hormones on Bile Acid Synthesis in Primary Cultured Rat Hepatocytes....Pages 143-148
Suppression of Killer T Cell Induction by Prodigiosin 25-C....Pages 149-154
Immunopotentiator from Bifidobacterium Adolescentis ....Pages 155-164
Immunosuppressive Properties of FK-506....Pages 165-169
Enhancement of Antibody Production and Cell Proliferation by Marine Bioactive Substances....Pages 171-176
Effect of a dialyzate of spinach on the chromosomal aberration induced by mutagens in cultured Chinese hamster cells....Pages 177-181
Suppression of Melanin Production in a Mouse B16 Melanoma 4A5 Cell Line by a Spinach Extract....Pages 183-188
Effect of Spinach Extracts on the Proliferation and on Some Phenotypic Characteristics of Human Myeloid Leukemia Cell Lines....Pages 189-193
Sustained Inducibility of Cytochrome P450 Activity in Rat Hepatocytes Cultured in a Serum-Free Medium....Pages 195-201
Biosynthesis of Protein Products by Animal Cells - Are Growth and Non-Growth Associated Concepts Valid or Useful?....Pages 203-213
Use of Liquid Medium Concentrates to Enhance Biological Productivity.....Pages 215-222
Optimisation of α-Interferon Expression of Namalwa Cells....Pages 223-230
Changes in the Glycosylation of Interferon-γ during Culture....Pages 231-235
Growth Control of Hybridoma Cultures by Adding or Depriving Interleukin-6, Caffeine, Amino Acids, Growth Factors, or Antisense C-myc....Pages 237-242
Chemostat Culture Studies on CHO Cells Producing Interferon-γ....Pages 243-250
Chinese Hamster Ovary (CHO) Cell Growth and Recombinant Protein Production in Serum-Free Media....Pages 251-257
Mechanism of IL-10 Production by a CD8 + T Cell Clone....Pages 259-266
Generation of CHO Cell Mutants for Growth Control....Pages 267-272
Removability and Permeability of DNA in a Solution by Cuprammonium Regenerated Cellulose Hollow Fiber (BMM TM ) for Process Validation of Purification Process of Bio-Drugs....Pages 273-279
Principles and Methods of Process Validation Studies for Virus Removal and Inactivation....Pages 281-288
Combination of Virus Removal and Inactivation for the Process Validation of Bio-Drugs....Pages 289-294
A New, Validated Method for the Destruction of Conventional Viruses and Unconventional Infectious Agents (e.g. BSE and Scrapie)’ in Proteins and Sera....Pages 295-300
Amino Acid Metabolism of a High Density Perfusion Culture....Pages 301-307
Kinetics of Cell Metabolism and Antibody Production in High Concentration Perfusion Cultures....Pages 309-324
Growth Yields of Hybridoma Cells and Monoclonal Antibody Production in High Density Culture....Pages 325-333
Towards a Strategy for High Density Cultures of Vero Cells in Stirred Tank Reactors....Pages 335-340
50L Scale Perfusion Culture of Hybridoma Cells by Gravitational Settling for Cell Separation....Pages 341-346
Production of Monoclonal Antibodies with a Radial-Flow Bioreactor....Pages 347-353
Factors Affecting the Yield of a Baculovirus Insect Cell System by Hollow-fiber Culture....Pages 355-360
High-Density Animal Cell Culture by Gas Sparging....Pages 361-367
Non-Woven Fabrics for Immobilizing Mammalian Cells....Pages 369-374
Statistical Design of Culture Medium for Recombinant Interferon-γ Production....Pages 375-381
Medium Optimization for Perfused Culture to Provide High Product Titer....Pages 383-390
eRDF Medium Enhances Antibody Production of IgG and IgM Secreting Hybridomas.....Pages 391-396
Serum-Free Media for Large-Scale Suspension Cultures....Pages 397-401
Suspension Culture of Mouse Hybridoma HB8852 Cells with a Protein-Free Medium....Pages 403-408
Improved Monoclonal Antibody Production via Controlled Feeding Strategies during Fedbatch Cultures of a Hybridoma Cell Line Utilizing Protein Free Media....Pages 409-415
Prediction of Disruption of Animal Cells by Laminar and Turbulent Flows....Pages 417-423
Fed-Batch Propagation of Mouse Hybridoma and Human Lymphoblastoid Cell Lines (LCLs)....Pages 425-431
Bioreactor with a Radial-flow Non-woven Fabric Bed for Animal Cell Culture....Pages 433-442
Partial Cell Culture Medium Recirculation Experience in Pilot Scale....Pages 443-446
Adaptation to Stirred Culture - A Study of a Moderately Shear Sensitive Cell Line (PQXB1/2).....Pages 447-454
Evaluation of optimal process parameters for the membrane dialysis bioreactor with integrated radial-flow fixed bed....Pages 455-462
High Cell Density Perfusion Culture of Hybridoma Cells for Production of Monoclonal Antibodies in the Celligen Packed Bed Reactor....Pages 463-469
Metabolic parameters of a hybridoma cell line in batch and continuous cultivation....Pages 471-478
Development of a New Disposable Culture System for Human Lymphokine-Activated Killer (LAK) Cells....Pages 479-486
New Method for Observing Cells Inside a Macroporous Microcarrier....Pages 487-491
Production of an α-Amidating Enzyme (α-AE) in Recombinant CHO Cells....Pages 493-499
Development of Instrumentation and Control Strategy in Bioreactor Culture of Hybridoma Cells....Pages 501-507
Effect of Dissolved Oxygen Concentration on Growth of Mouse-Mouse Hybridoma....Pages 509-514
Isolation of Autocrine Growth Factors Producing Mouse Hybridoma Cell Lines....Pages 515-520
Production of Multisubunit Particles for Use as Vaccines Using the Baculovirus Expression Vector System (BEVS)....Pages 521-528
Spontaneous Apoptosis in Mouse Hybridoma Culture....Pages 529-534
Cell Cycle Dynamics of Antibody Formation in Hybridomas....Pages 535-541
Effect of the Cell-Cycle Phase on Hybridoma Culture....Pages 543-549
Physiological Aspects of Scaling Up and Monitoring....Pages 551-556
A Comparative Study of the Immunopotentiating Activity of Adjuvant Formulations Containing Glycopeptides....Pages 557-564
Establishment and Characterization of Monoclonal Antibodies against Fish Virus ( Iridoviridae )....Pages 565-571
The Production of Recombinant Antibodies Using the Glutamine Synthetase (GS) System....Pages 573-577
Generation of Supranatural Antibodies by Modifying the L Chains: Cell Hybridization and Glycotechnology....Pages 579-583
Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production....Pages 585-594
The Use of Artificial Intelligence to Enhance the Accuracy of Hybridoma Growth and Metabolism....Pages 595-600
Immunoglobulin Production Stimulating Factor (IPSF) Derived from Namalwa Cells....Pages 601-607
One-Step Purification of F(ab’) 2 Fragments of Mouse Monoclonal Antibodies (Immunoglobulins G1 and M) by Hydrophobic Interaction HPLC....Pages 609-616
Application of Anti-Tetanus Human Monoclonal Antibodies....Pages 617-623
Genetically Engineered Antibodies....Pages 625-639
Applications of Human Monoclonal Antibodies to Various Clinical Uses....Pages 641-646
Reactivity of Human Monoclonal Antibody BD9-D12 to Cancer Cells and Tissues....Pages 647-652
Cell Culture in Europe: A Review of Today’s Market for Cell Culture Products and Services....Pages 653-658
Back Matter....Pages 659-668