Animal Cell Culture Methods

Content: 
Series Editors
Page ii

Edited by
Page iii

Copyright Page
Page iv

Contributors
Pages xi-xii

Preface
Pages xiii-xiv
Jennie P. Mather, David Barnes

Principles of Cell Culture
Pages 1-2

Chapter 1 Animal Cell Culture Equipment and Techniques Original Research Article
Pages 3-17
Angela Helmrich, David Barnes

Chapter 2 Making Informed Choices: Medium, Serum, and Serum-Free Medium How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create Original Research Article
Pages 19-30
Jennie P. Mather

Chapter 3 Cell Line Availability: Where to Get the Cell Lines You Need Original Research Article
Pages 31-47
Robert J. Hay

Chapter 4 Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination Original Research Article
Pages 49-65
Carolyn Kay Lincoln, Michael G. Gabridge

Establishing Cell Lines
Pages 67-68

Chapter 5 Immortalization by Gene Transfection Original Research Article
Pages 69-91
Yoshinori Katakura, Shahabuddin Alam, Sanetaka Shirahata

Chapter 6 Establishment of Mammalian Testicular Cell Lines Original Research Article
Pages 93-110
Marie-Claude C. Hofmann, Jose Luis Millan

Chapter 7 Cell Hybridization, Hybridomas, and Human Hybridomas Original Research Article
Pages 111-145
Sanetaka Shirahata, Yoshinori Katakura, Kiichiro Teruya

Chapter 8 Establishing Human Glioma-Derived Cell Lines Original Research Article
Pages 147-165
Manfred Westphal, Hildegard Meissner

Chapter 9 Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells Original Research Article
Pages 167-186
Ronghao Li

Chapter 10 Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms Original Research Article
Pages 187-201
Christopher J. Bayne

Chapter 11 Cell Line Characterization and Authentication Original Research Article
Pages 203-216
Joseph Kaplan, Bharati Hukku

Specialized Culture Techniques
Pages 217-218

Chapter 12 Laboratory Scaleup of Cell Cultures (0.5-50 Liters) Original Research Article
Pages 219-227
Jennie P. Mather

Chapter 13 Cell Synchronization Original Research Article
Pages 229-249
Gary F. Merrill

Chapter 14 Measurement of Cell Death Original Research Article
Pages 251-264
Deryk T. Loo, Jill R. Rillema

Chapter 15 Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death Original Research Article
Pages 265-278
Alison Moore, Christopher J. Donahue, Kenneth D. Bauer, Jennie P. Mather

Chapter 16 Embryonic Stem Cells, Creating Transgenic Animals Original Research Article
Pages 279-293
Melinda Pirity, Anna-Katerina Hadjantonakis, András Nagy

Microscopy and Morphology
Page 295

Chapter 17 Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture Original Research Article
Pages 297-311
David M. Phillips

Chapter 18 Indirect Immunofluorescence Microscopy in Cultured Cells Original Research Article
Pages 313-332
Sally P. Wheatley, Yu-li Wang

Chapter 19 Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and in Situ Ligand Binding Original Research Article
Pages 333-351
Teresa K. Woodruff

Index
Pages 353-361

Volumes in Series
Pages 363-368