Aging: Methods and Protocols

Front Matter ....Pages i-xii
 Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform  (Ruofan Yu, Myeong Chan Jo, Weiwei Dang)....Pages 1-6
 Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches  (Adam B. Cornwell, Andrew V. Samuelson)....Pages 7-27
 A Simple Apparatus for Individual C. elegans Culture  (William E. Pittman, Drew B. Sinha, Holly E. Kinser, Nisha S. Patil, Eric S. Terry, Isaac B. Plutzer et al.)....Pages 29-45
 Analysis of Drosophila melanogaster Lifespan  (Gary N. Landis, Devon Doherty, John Tower)....Pages 47-56
 Mouse Fitness as Determined Through Treadmill Running and Walking  (Joseph C. Reynolds, Changhan Lee)....Pages 57-65
 Human Population Genetics in Aging Studies for Molecular Biologists  (Brendan Miller, Amin Haghani, Jennifer Ailshire, T. Em Arpawong)....Pages 67-76
 Design and Analysis of Pharmacological Studies in Aging  (Khalyd J. Clay, Michael Petrascheck)....Pages 77-89
 Methods for Assessing Fertility in C. elegans from a Single Population  (Chia-An Yen, Sean P. Curran)....Pages 91-102
 Metabolic Assessment of Lipid Abundance and Distribution  (James D. Nhan, Sean P. Curran)....Pages 103-110
 Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography–Mass Spectrometry  (Elizabeth C. Pino, Alexander A. Soukas)....Pages 111-123
 Assessing Insulin and Glucose Tolerance in the Aging Mouse  (Roberta Buono, Sebastian Brandhorst)....Pages 125-129
 High-Throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome  (Fan Zhang, Jessica L. Weckhorst, Adrien Assié, Anastasia S. Khodakova, Mario Loeza-Cabrera, Daniela Vidal et al.)....Pages 131-144
 Measurements of Innate Immune Function in C. elegans  (Kyle J. Foster, Deborah L. McEwan, Read Pukkila-Worley)....Pages 145-160
 Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging  (Ryan Lu, Nirmal K. Sampathkumar, Bérénice A. Benayoun)....Pages 161-170
 Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells  (Mintie Pu, Siu Sylvia Lee)....Pages 171-175
 Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age  (Rachel Kaletsky, Coleen T. Murphy)....Pages 177-186
 Asessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans  (Jessica T. Chang, Malene Hansen, Caroline Kumsta)....Pages 187-200
 Assay Development and Measurement of the Aging Biomarker Humanin  (Brendan Miller, Junxiang Wan)....Pages 201-209
 Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Disease  (Amy Christensen, Christian J. Pike)....Pages 211-221
 Multimodal Imaging of Cerebral Microhemorrhages and White Matter Degradation in Geriatric Patients with Mild Traumatic Brain Injury  (Maria Calvillo, Di Fan, Andrei Irimia)....Pages 223-236
 Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach  (Hongqiao Zhang, Natalie Lyn, Amin Haghani, Henry Jay Forman)....Pages 237-244
 Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging  (Juan Manuel Iglesias-Pedraz, Lucio Comai)....Pages 245-257
 Integrating Longitudinal Population Studies of Aging in Biological Research  (Amin Haghani, Brendan Miller, T. Em Arpawong, Jennifer Ailshire)....Pages 259-273
Back Matter ....Pages 275-277