Advancing Methods for Biomolecular Crystallography

Front Matter....Pages i-xiii
The Zen of Model Anomalies – Correct Most of Them. Treasure the Meaningful Valid Few. Live Serenely with the Rest!....Pages 1-10
Total Chemical Protein Synthesis for the Determination of Novel X-ray Structures by Racemic Protein Crystallography....Pages 11-22
Crystal Pathologies....Pages 23-31
Crystallizing Membrane Proteins for Structure-Function Studies Using Lipidic Mesophases....Pages 33-46
Searching for Needles in Haystacks: Automation and the Task of Crystal Structure Determination....Pages 47-57
Data Processing: How Good Are My Data Really ?....Pages 59-68
Radiation Damage in Macromolecular Crystallography: What Is It and Why Do We Care?....Pages 69-77
Elemental Analysis of Proteins by Proton Induced X-ray Emission (microPIXE)....Pages 79-89
X-rays-Induced Cooperative Atomic Movement in a Protein Crystal....Pages 91-103
Everything Happens at Once – Deconvolving Systematic Effects in X-ray Data Processing....Pages 105-112
Extending the Reach of Molecular Replacement....Pages 113-122
Phasing Through Location of Small Fragments and Density Modification with ARCIMBOLDO....Pages 123-133
SAD/MAD Phasing....Pages 135-149
Macromolecular Phasing: Solving the Substructure....Pages 151-157
Advanced Applications of Shelxd and Shelxe....Pages 159-167
Use of a Weak Anomalous Signal for Phasing in Protein Crystallography: Reflection from Personal Experience....Pages 169-180
 Ab Initio Low Resolution Phasing....Pages 181-192
Model-Building and Reduction of Model Bias in Electron Density Maps....Pages 193-203
Using Coot to Model Protein and Ligand Structures Using X-ray data....Pages 205-209
Crystallographic Structure Refinement in a Nutshell....Pages 211-219
Crystallographic Maps and Models at Low and at Subatomic Resolutions....Pages 221-230
Recent Advances in Low Resolution Refinement Tools in REFMAC5 ....Pages 231-258
High Resolution Macromolecular Crystallography....Pages 259-275
Publishing in Proteopedia: The Guide....Pages 277-295
Proteolysis, Complex Formation and Conformational Changes Drive the Complement Pathways....Pages 297-307
Monoamine Oxidase Inhibitors: Diverse and Surprising Chemistry with Expanding Pharmacological Potential....Pages 309-312
Structure of the Eukaryotic Ribosome: Tips and Tricks....Pages 313-320
Neutron Protein Crystallography. How to Proceed the Experiments to Obtain the Structural Information of Hydrogen, Protons and Hydration in Bio-macromolecules....Pages 321-330
Coherent Diffraction and Holographic Imaging of Individual Biomolecules Using Low-Energy Electrons....Pages 331-342
Structure Analysis of Biological Macromolecules by Small-Angle X-ray Scattering....Pages 343-351
Protein Structure Modeling with Rosetta: Case Studies in Structure Prediction and Enzyme Repurposing....Pages 353-362