Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology

Front Matter....Pages i-x
Carbon—14- and Tritium-Labeled DPN and TPN....Pages 1-12
Synthesis and Metabolism of Glucose-3-H 3 and Glucose-4-H 3 ....Pages 13-21
Use of Vinyl Magnesium Bromide in the Synthesis of Labeled Compounds: New Preparations of Glycerol-1-C 14 and Glyceric Acid-1-C 14 ....Pages 23-30
C 14 -Labeled Cardiac Glycosides....Pages 31-36
A Simple Method of Preparing Labeled Benzene....Pages 37-44
Synthesis of 35 S-p-Toluene Sulfonic Anhydride (Tosan) of High Specific Activity....Pages 45-55
The Correlation between Structure and Tritium Incorporation During Labeling by the Wilzbach Method....Pages 57-59
Synthesis of 32 P-, 14 C-, and 3 H-Labeled Lecithins and Their Use in Studies on Lipid Metabolism....Pages 61-67
Improved Methods for Liquid Scintillation Assay of (A) C 14 -Compounds on Paper Chromatograms and (B) C 14 -Protein....Pages 69-79
Quenching Correction in Liquid Scintillation Counting....Pages 81-94
Quench Monitoring and Efficiency Calibration Through External Standardization....Pages 95-106
Comparison of Various Procedures for the Simultaneous Measurement of 32 P and 45 Ca in Inorganic Salts by Liquid Scintillation Counting....Pages 107-117
Problems of Liquid Scintillation Counting on Studies of Metabolic Balances....Pages 119-129
Electron Microscope Autoradiography....Pages 131-137
A New Method of Macroautoradiography....Pages 139-147
Thin Layer Fluorography of Tritium-Labeled Compounds....Pages 149-155
Thin Layer Autoradiographic Electrophoresis of Tritium-Labeled Compounds in Buffered Photographic Gelatin....Pages 157-159
Concentration Profile in Paper Chromatography Investigations of a Labeled Substance....Pages 161-172
The Importance of Isotope Fractionation in the Clinical Application of Labeled Aldosterone....Pages 173-179
Recent Investigations on Cholesterol Metabolism: Critical Evaluation of Radiochromatography and Estimation Procedures....Pages 181-198
Isotope Studies on Citrate-Condensing Enzyme....Pages 199-209
Rapid Inhibitor-Induced Changes in the C 14 -Labeled Compounds Formed by the Photosynthetic Carbon Reduction Cycle....Pages 211-221
Application of Double Isotope Labeling to the Study of the Metabolism of Ascites Tumor Cells under the Influence of Glyceraldehyde....Pages 223-231
Nucleic Acid Metabolism during Antibody Synthesis in Vitro....Pages 233-239
The Biosynthesis of Prostaglandin-E 1 Studied with Specifically 3 H-Labeled Precursors....Pages 241-242
The Use of Labeled Substrates in the Study of Isoprenoid Degradation....Pages 243-256
Use of Different Isotopes in the Study of Cell Differentiation....Pages 257-273
Degradation of C 14 -Labeled Thymidine and its Halogenated Analogues in Man....Pages 275-282
Quantitative Measurement of Transplantation Immunity....Pages 283-284
Studies of the Secretion and the Metabolism of Steroid Conjugates: Synthesis, Analysis, and Use of Doubly Labeled Compounds....Pages 285-299
Countercurrent Distribution Combined with Liquid Scintillation Counting of Double-Labeled Water-Soluble Radioactive Material in the Study of the Metabolism of Steroid Conjugates....Pages 301-311
Conjugation (Detoxification) of C 14 -Labeled Cholic Acid in Human Liver Homogenates as a Test of Liver Function....Pages 313-320
Atmospheric Bomb Radiocarbon as a Tracer in Human Beings....Pages 321-329
Back Matter....Pages 331-333