Advances in Tracer Methodology: Volume 1

Front Matter....Pages i-xi
Opening Remarks by Chairman....Pages 1-3
The Gas Exposure Technique for Tritium Labeling....Pages 4-11
Experiences with Tritiated Compounds Prepared by Exposure to Tritium Gas....Pages 12-17
Reaction of Unsaturated Organic Compounds with Tritium Gas....Pages 18-27
Tritium Gas Exposure Labeling....Pages 28-31
Some Experiences with Labeling Sugars by Tritium Gas Exposure....Pages 32-35
Some Experiences with Labeling Nucleotides....Pages 36-41
Correlation of Efficiency of Labeling with Chemical Constitution....Pages 42-45
The Radiation-Induced Addition of Tritium to Unsaturated Systems....Pages 46-49
Tritium Labeling by Other Methods....Pages 50-51
Tritium Recoil Labeling of Linseed Oil....Pages 52-55
Preparation of Tritium-Labeled Paromomycin (Humatin) by Fermentation in a Medium Containing Tritiated Water....Pages 56-59
Some Aspects of Stereoselectivity in the Introduction of Tritium into Steroids....Pages 60-63
Radiation Self-Decomposition of Labeled Compounds....Pages 64-68
Liquid Scintillation Counting of Tritium....Pages 69-75
Recent Developments in Simultaneous C 14 and Tritium Counting....Pages 76-85
Determination of Liquid Scintillation Counting Efficiency by Pulse-Height Shift....Pages 86-92
A New Technique for Counting Aqueous Solutions in the Liquid Scintillation Spectrometer....Pages 93-105
Liquid Scintillation Counting of Tritium in Suspended Materials....Pages 106-112
Counting C 14 O 2 with a Liquid Scintillation Counter....Pages 113-114
Liquid Scintillation Counting of C 14 O 2 in Aqueous Carbonate Solutions....Pages 115-118
Determination of Radioactive Sulfur in Biological Materials....Pages 119-120
Scintillation Counting of C 14 -Labeled Paper Chromatograms....Pages 121-126
Simultaneous Measurement of C 14 and H 3 during Gas-Liquid Chromatography....Pages 127-146
Monitoring Gas Chromatography for H 3 - and C 14 -Labeled Compounds by Liquid Scintillation Counting....Pages 147-152
Application of Whole-Body Liquid Scintillation Counters to Pharmacological Studies....Pages 153-159
A Comparison of Ionization-Chamber and Liquid-Scintillation Methods for Measurement of Beta Emitters....Pages 160-166
Tritium Measurement Using Ionization Chambers....Pages 167-177
Gas Counting of Tritium....Pages 178-182
Vapor Phase Proportional Counting....Pages 183-184
Determination of Tritium and C 14 in Biological Samples by Rapid Combustion Techniques....Pages 185-191
Parr Bomb Combustion of Tissues for C 14 and Tritium Analysis....Pages 192-194
Recent Advances in Instrumentation....Pages 195-200
Tritium Health Physics Considerations....Pages 201-202
Design and Analysis of Tracer Experiments....Pages 203-204
Design and Analysis of Tracer Experiments—Biochemical Aspects....Pages 205-209
Experimental Design of Turnover Measurement Studies in the Unsteady State: Biosynthesis of Macromolecules....Pages 210-216
Measuring Turnover Rates in the Nonsteady State....Pages 217-221
Tritium in Biochemical Studies....Pages 222-226
Special Features of Tritium as a Tracer in Industrial Research....Pages 227-233
Some Uses of Tritium-Labeled Compounds in Pharmaceutical Research....Pages 234-246
Metabolism of DL-Epinephrine-7-H 3 D-Bitartrate....Pages 247-256
Application of Tritium in the Determination of Gibberellins....Pages 257-262
New Developments in the Use of Radioisotopes in Reservoir Engineering....Pages 263-264
Use of Radioisotopes in Steroid Methodology....Pages 265-273
Metabolism Studies by Radiorespirometry....Pages 274-290
Autoradiography with Tritium....Pages 291-294
Some Uses of Tritium in Autoradiography....Pages 295-301
The Efficiency of Autoradiographic Stripping Film Applied to Tissue Sections Containing Tritiated Thymidine....Pages 302-308
Autoradiography of Whole Animals as an Experimental Tool in Pharmacological Research....Pages 309-313
Clinical Use of the Arm Counter in Blood Clearance Studies....Pages 314-325
Back Matter....Pages 327-332