Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991

Front Matter....Pages I-XX
Speech of the Minister of Justice of Rheinland-Pfalz (Germany) Peter Caeser at the occasion of the opening of the 14th Congress of the International Society of Forensic Haemogenetics....Pages 1-8
Front Matter....Pages 9-9
Some Considerations for Use of AMP-FLPs for Identity Testing....Pages 11-17
Forensic Use of Short Tandem Repeats via PCR....Pages 18-25
Automation of the Amplification and Sequencing of Mitochondrial DNA....Pages 26-28
The Development and Evaluation of New Genetic Markers for the Application of PCR to Forensic Casework....Pages 29-31
Investigations to Improve Allele Definition in the “Collagen 2A1” System (AMP-FLP)....Pages 32-34
Automated Analysis of Fluorescent Amplified Fragment Length Polymorphisms for DNA Typing....Pages 35-37
Sex Identification of Forensic Samples Using PCR Analysis for the Presence of Y-Chromosome Specific DNA Sequences....Pages 38-40
Automation of DNA Profiling by Fluorescent Labelling of PCR Products....Pages 41-43
PCR Typing Including High Resolution Gel Electrophoresis Reveals new Alleles in the COL2Al VNTR....Pages 44-46
The Usefulness of Chelating Resins for DNA Extraction from Forensic Material Prior to PCR Amplification....Pages 47-49
The Isolation of DNA for Forensic PCR Analysis. An Evaluation of Available Methodologies....Pages 50-52
Detection of Three Different VNTR’s by DNA-Amplification....Pages 53-56
Validation of the Use DNA Amplification for the Analysis of Forensic Samples by Comparison with Tests Using Non-Amplified DNA Polymorphisms....Pages 57-59
Investigations on the Forensic application of 4 AMPFLP systems....Pages 60-63
SDS-PAGE Typing of HLA-DQA1 and pMCT118 after PCR Amplification....Pages 64-66
Gene frequencies of APOB alleles in a sample of random Italian individuals (Central and Southern Italy)....Pages 67-69
Study of the HLA DQα polymorphism in the population of Catalonia (Spain)....Pages 70-71
Use of PCR for Forensic Analysis of DNA from Formaldehyde fixed and Paraffin embedded Human Tissues....Pages 72-74
Comparison of Population data using 3 AMPFLP systems....Pages 75-77
Front Matter....Pages 9-9
The Amplified Fragment Length Polymorphism Study of Locus pMCT118 and Its Application in Forensic Biology....Pages 78-80
DNA typing from formalin-fixed, paraffin-embedded tissues....Pages 81-83
HLA-DQA1 and HLA-DPB1 gene polymorphisms in the Japanese populations....Pages 84-86
The Study of apoB Locus Apm-FLP and Its Application in Forensic Sciences....Pages 87-89
Application of HLA-class II Genotyping by the Modified PCR-RFLP Method to the Forensic Science....Pages 90-92
Simultaneous DNA analysis of HLA-DPB and -DQB loci from single hairs: a criminal case report....Pages 93-95
Sex Determination by Genomic Dot Blot Hybridization and HLA DQα Typing by PCR from Fixed Tissues....Pages 96-98
HLA DQα Typing of Human Fingernails....Pages 99-101
Identification of Fire Victims by Using DNA Amplification (PCR)....Pages 102-105
Polymerase Chain Reaction: Typing of DNA Isolated from Various Forms of Biological Evidence....Pages 106-108
Analysis of D1S80 (pMCT 118) Locus Polymorphism in an Italian Population Sample by the Polymerase Chain Reaction....Pages 109-111
Sex determination in bloodstains and single hairs....Pages 112-114
Analysis of forensic stains via PCR amplification of polymorphic simple (gata) n repeats....Pages 115-117
Species identification by polymerase chain reaction and direct sequencing....Pages 118-120
Front Matter....Pages 121-121
Paternity Investigations Based on DNA-Analysis Only....Pages 123-125
Achievement of Interlaboratory Uniformity — A Summary of Work Carried out by the EDNAP Group....Pages 126-128
Cloning and Characterisation of Novel Single Locus Probes for Forensic Purposes....Pages 129-131
The Use of a Chemiluminescent Detection System for Paternity and Forensic Testing. Verification of the Reliability of the Oligonucleotide-Probes Used for Genetic Analysis....Pages 132-136
A Multi-locus Probe for Human DNA Fingerprinting Based on chi -like Sequences....Pages 137-139
The Effects of Using Different Molecular Weight Markers in DNA Profiling....Pages 140-142
Front Matter....Pages 121-121
Influence of Agarose Concentration and Electric Field Strength on the Separation of DNA-Fragments During Electrophoresis....Pages 143-145
Size Calculations of Restriction Fragments: Comparison Between Two Laboratories....Pages 146-148
Matching Criteria for Paternity Testing with VNTR Systems....Pages 149-152
Computer-Aided Fragment Size Determination of Single Locus DNA Probes....Pages 153-155
Chemiluminescent detection of single locus and multilocus hybridization patterns....Pages 156-158
Precision and Accuracy in the Analysis of VNTR Polymorphisms for Paternity Testing....Pages 159-162
A Simple Method to Prevent Inhibition of Taq Polymerase and Hinfi Restriction Enzyme in DNA Analysis of Stain Material....Pages 163-165
Optimal Size Calling Methods for Electrophoretic Analysis Utilizing Internal Size Standards....Pages 166-168
Extraction of DNA from Coagulated Blood Samples....Pages 169-171
Investigation of variation in fragment size determinations found when using single locus DNA probes....Pages 172-174
Optimization of the Digoxigenin/Chemiluminescence Method for the VNTR Detection....Pages 175-178
Paternity Analysis Using the Multilocus DNA Probe MZ 1.3....Pages 179-181
Analysis of Australian, Black, Caucasian, Chinese and Amerindian Populations with Hypervariable DNA Loci....Pages 182-183
The Application of DNA-Polymorphisms in Paternity Testing....Pages 184-186
Allele Frequency in the Population of Spain Using Several Single Locus Probes....Pages 187-189
DNA Fingerprints of Families from Bejsce/South-East Poland....Pages 190-191
Paternity Testing with Five VNTR Systems in Danes....Pages 192-194
Comparison of Population Genetics of the Single Locus Probes pS194 and pL427-4....Pages 195-197
Comparison of Minisatellite DNA Probes and Blood Group, Protein, and Enzyme Markers in Paternity Cases....Pages 198-200
Allele frequency distribution of two VNTR polymorphisms (YNH24/ D2S44; Alpha globin 3′HVR/D16) in Italy....Pages 201-203
Front Matter....Pages 121-121
DNA-Profiling with pHINS310, pMUC7, pMR24/1, pYNH24 and pMS43a for Paternity Testing....Pages 204-206
Frequency Databases for the DNA Probes MS1, MS31, MS43A, and YNH24, Derived from Caucasians, and Afro-Caribbeans in the London Area....Pages 207-209
An Evaluation of Single Locus Probes in Casework....Pages 210-212
On DNA typing of hard tissues....Pages 213-215
Use of the Minisatellite Probe MZ 1.3 for Identification and Relation of Dismembered Corpses....Pages 216-218
RFLP in Conjunction with Anatomical Traits in Individualisation of Bone....Pages 219-221
Application of DNA Fingerprinting to Problematical Paternity Cases....Pages 222-224
Application of conventional polymorphisms and single locus DNA probes in cases of disputed paternity....Pages 225-227
MS1, MS31 and MS43A Single Locus Probes: A Preliminarystudy in the Basque Population and its Application in Paternity Testing....Pages 228-230
DNA Fingerprinting with Probes 33.15 and 33.6 in Population from the Basque Country....Pages 231-233
Casuistic: The Use of DNA-Fingerprinting in Cases of Affiliation Without Mother....Pages 234-236
Results of DNA Analysis from Six Forensic Science Laboratories in Germany....Pages 237-239
Allele Frequencies for Five Different Single Locus Probes in a Population of South-West Germany....Pages 240-242
Distribution of variable number of tandem repeat (VNTR) DNA polymorphism at D2S44 locus in Tuscany (Italy)....Pages 243-245
Experiences with Six Single Locus Probes in Paternity Testing....Pages 246-248
Detection of DNA polymorphisms by using α satellite probes: Application to the forensic identification....Pages 249-251
Population genetic studies of six hypervariable DNA-Loci....Pages 252-254
Minisatellite DNA Probe MZ1.3: Band Sharing Rates among Siblings and the Part of Informative Bands among Children....Pages 255-257
DNA Fingerprinting in Paternity Testing in Lithuania....Pages 258-259
DNA typing in forensic casework in Norway: strategies and experiences....Pages 260-262
Front Matter....Pages 121-121
Determination of Incest in Forensic Casework Using Multi-locus DNA Profiling....Pages 263-266
Front Matter....Pages 267-267
The Robustness of Models for Evaluating Patterns of DNA Multi-Locus Probes....Pages 269-276
Comparison of Different Methods for the Calculation of Indices of Paternity....Pages 277-284
How to Deal with Mutations in DNA-Testing....Pages 285-287
The Relationship of the HLA Phenotype Frequency of the Alleged Father to the Resulting Paternity Index in Caucasian Non-Exclusion Paternity Cases....Pages 288-290
DNASYS: A User-Friendly Computer Program for Evaluating Single Locus Probe Data in Forensic Casework....Pages 291-294
Series Sexual Crimes Identified by a DNA Computerised Database....Pages 295-297
Experiences with a Computerised Database of DNA Profiles in Forensic Casework....Pages 298-300
Front Matter....Pages 301-301
Genetic and Molecular Aspects of the Human Red Cell Acid Phosphatase Polymorphism....Pages 303-305
A de novo Mutation in the Alpha-1-Antitrypsin Gene detected in a Case of disputed Paternity by DNA Sequence Analysis....Pages 306-308
Isoelectric Focusing of Inter-Alpha-Trypsin Inhibitor (ITI)....Pages 309-310
Human ZN-Alpha 2-Glycoprotein Phenotyping in Several Populations....Pages 311-313
Comparative Typing of Orosomucoid Variants and Proposal for a New Nomenclature....Pages 314-316
Intragenic Recombination within the Alpha-1-Antitrypsin Locus....Pages 317-319
Blotting Techniques for the Detection of Protein Polymorphisms in Stains....Pages 320-328
Two-Dimensional Isoelectric Focusing Analysis of Rare and Silent Esterase D Types. Description of a New ESD Variant Phenotype....Pages 329-331
A Simple Technique for the Determination of GGTP Types....Pages 332-334
New Variation in Low-Sulfur Keratins Detected by Hybrid Isoelectric Focusing (HIEF)....Pages 335-337
The Influence of Infused Erythrocytes on the Detection of Individual Membrane-, Enzyme- and DNA- Systems....Pages 338-340
Serum Protein Typing/-Subtyping by IEF in PAG....Pages 341-343
Front Matter....Pages 301-301
Dot Blot Immunoassay for Detection of HLA Antigens in Forensic Stains....Pages 344-346
The use of microtiter techniques for the determination of red blood cell phenotypes in paternity testing....Pages 347-349
D u detection by an automated direct agglutination method that equals detection by indirect antiglobulin test....Pages 350-352
Absorption-Elution Test for ABO-Determination of Secretor and Nonsecretor Saliva Stains....Pages 353-355
Determination of ORM Phenotypes using gels PRECOTES 4–6 R and PhastSystem™....Pages 356-358
Fast Determination of TF Phenotypes Using Minigel Gradient 4–6.5 Modificated....Pages 359-361
The Use of Microtiter Plates and the Apparatus Dynatech for Automation of the Routine Determination of ABO-Group from Body Fluids (or Stains) and Hair....Pages 362-363
PI, C2, GC, ATIII, PLG Typing in Bloodstains by Hybrid Isoelectric Focusing (HIEF)....Pages 364-366
Parentage Testing Using DBP Subtyping in South African (SA) Populations....Pages 367-369
Iso-Electric Focusing Study of Serum Proteins (GC, TF, PI and ORM) in Four Endogamous Groups of Maharashtra, Western India: Application in Paternity Testing....Pages 370-372
PGM1 System: A Rare Allele and an Intragenic Recombination in Two Cases of Disputed Paternity....Pages 373-375
Genetic markers (HP, TF, GC and PI) in two Polish population samples Preliminary report....Pages 376-378
Haptoglobin Subtypes in Lower Saxony (Germany)....Pages 379-381
C4 Phenotype and Gene Distribution in a Population of Eastern Lombardy (Italy)....Pages 382-384
Distribution of Transferrin (Tf), Red Cell Acid Phosphatase (EAP), Esterase D (EsD) and Group Specific Component (Gc) Phenotypes in China....Pages 385-388
Null and Rare Alleles in Paternity Testing....Pages 389-390
Comparative Subtyping of ACP-1, PGM-1 and ESD in Human Placenta and Cord Blood by Isoelectric Focusing: Practical Considerations of Forensic Significance....Pages 391-393
Distribution of Adenosine Deaminase (EC 3.5.4.4) Phenotypes in a Series of HIV-Seropositive Patients....Pages 394-396
Determination of C1R Types in Bloodstains....Pages 397-399
Coagulation Factor XIIIB Phenotyping in a Japanese Population and in Bloodstains....Pages 400-402
Front Matter....Pages 301-301
Formal Genetic Data on ORM1 Subtypes....Pages 403-406
Species identification from tissue particles using lectin- and immuno-histochemical methods....Pages 407-409
Polymorphism of Plasminogen in Sardinia (Italy)....Pages 410-412
Haptaglobin Subtypes in a Population from South West Germany....Pages 413-415
Plasma Protein Polymorphism in HIV-Seropositive Patients: GC- and TF*C-Subtypes and PI-System....Pages 416-418
Determination Of PGM, EsD, GLO (1) and EAP Polymorphs from Human Dental Pulp....Pages 419-421
Haptoglobin Subtyping by Polyacrylamide Gel Isoelectric Focusing of Serum, Hemolyzed Blood and Bloodstains....Pages 422-424
Detection of Blood Group H Antigens of Red Cells, Blood and Saliva Stains, and Hairs by Anti-H Reagents....Pages 425-427
Evaluation of Sperm Specific Lactate Dehydrogenase Isoenzyme C4 (LDH C4). Application to Semen Detection in Stains....Pages 428-430
PGM1 Subtyping by Isoelectric Focusing (IEF) in Parentage Testing in South African (SA) Populations....Pages 431-433
Orosomucoid (ORM) Phenotyping by Isoelectric Focusing in Immobilized pH-Gradient Followed by Immunoblotting....Pages 434-436
ABO Blood Grouping and Species Identification of Bloodstains by Sandwich ELISA Using Monoclonal Antibody Specific for Human Erythrocyte Band 3....Pages 437-439
Monoclonal Antibodies to Blood Group Substances in Vaginal Secretions....Pages 440-442
Gc in Human Saliva Stains....Pages 443-445
Immunoblotting and Immunofixation Techniques for Subtyping Gc in Old Bloodstains and Semen Stains....Pages 446-448
Old Bloodstain and Semen stain Characterization in the Transferrin Typing System Using Minigels and PhastSystem....Pages 449-451