Advances in Applied Biotechnology: Proceedings of the 3rd International Conference on Applied Biotechnology (ICAB2016), November 25-27, 2016, Tianjin, China

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 Isolation and Characterization of a Virulent Phage H6 Infecting Lactobacillus brevis from the Fermented Chinese Cabbage  (Kechong Huang, Tian Zhang, Hongjiang Yang)....Pages 3-12
 Molecular Cloning and Biochemical Characterization of Oligo-1,6-Glucosidases from Bacillus subtilis and Bacillus licheniformis  (Xiaoming Hao, Ke Cai, Zixing Dong)....Pages 13-20
 Transcriptomic Analysis of CYP Genes in Rhizopus nigricans and Identification of the Steroid 11α-Hydroxylase Candidate Genes  (Jianguo Zhao, Pengcheng Sui, Ruijie Wang, Ziyin Zhang, Fuping Lu, Zhengxiang Wang et al.)....Pages 21-27
 Identification of Genes Encoding Receptors for Six Pseudomonas aeruginosa Phages  (Jiajia You, Xiaoli Cui, Li Sun, Xiaojing Yang, Hongjiang Yang)....Pages 29-39
 High Efficiency Expression of Trehalose Synthase in Escherichia coli and Its Use in the Production of Trehalose  (Hong-Ling Liu, Rui-Ming Wang, Teng-Fei Wang)....Pages 41-58
 Functional Modification of the Substrate-Binding Site for Isomaltulose Production Based on Predicted Structure of Sucrose Isomerase from Pantoea dispersa UQ68 J  (Huijie Liu, Xueyan Xing, Fuping Lu, Yu Li)....Pages 59-68
 Construction of the Escherichia coli-Bacillus subtilis Shuttle Vector pBE2R and Identification of the Critical Residues Involved in the Autoprocessing of the Propeptide of the Alkaline Protease  (Run Wei, Xiao-mei Liang, Mei-juan Xuan, Hao-yu Yuan, Fu-ping Lu, Ming Li)....Pages 69-78
 Effects of ATF2 Overexpression with BAT2 Deletion on the Higher Alcohols and Esters in Beer Yeast  (Xiaoer Liu, Jia Xu, Li Pi, Cuiying Zhang, Dongguang Xiao)....Pages 79-87
 Study on a Staphylococcal Tat Signal Peptide Guided EGFP Translocation in E. coli  (Qiu-Xiang Zhou, Jun Zhang, Mei-Na Wang, Wen-Hao Yang, Jian Zhang, Qiang Gao)....Pages 89-95
 A Novel GH10 Xylanase Xyn13-3 from Alkaline Soil: Gene Cloning and Heterogenous Expression  (Haiyan Qiu, Zhongyuan Li, Hui Wang, Shuang Li, Tongcun Zhang)....Pages 97-103
 Exploitation of a KU70-Deficient Mutant for Improving Gene Deletion Frequency in Aspergillus niger  (Liu-hua Yin, Lan Zhang, Ling Liu, Hongfei Zhang, Li Hou, De-pei Wang)....Pages 105-115
 Biotransformation to Produce Boldenone by Pichia pastoris GS115 Engineered Recombinant Strains  (Rui Tang, Peilin Ji, Ying Yu, Xu Yang, Mengjiao Liu, Kaiyuan Chen et al.)....Pages 117-124
 The Prokaryotic Expression of Cyclin-Dependent Kinase 2, and the Establish of Its Inhibitor Screening System  (Yuan Yuan, Meile Gao, Huan Liu, Tingting Ruan, Weiran Xie, Meng Wu et al.)....Pages 125-132
 Effects of Heterologous Pyruvate Carboxylase Expression on Synthesis of l-Threonine in Escherichia coli  (Junpeng Wang, Yan Zhao, Tao Liu, Ting Wang, Chao Han, Qian Mao et al.)....Pages 133-143
 A Crystal Structure of the R-Amine Transaminase from Arthrobacter for Asymmetric Catalysis of Chiral Amines  (Lijun Guan, Nina Ji, Qinghong Meng, Xuejun Xie, Ye Zhou, Yijuan Cui et al.)....Pages 145-151
Front Matter ....Pages 153-153
 Optimization of Culture Medium for Rifamycin SV Production by Amycolatopsis kentuckyensis 22-187  (Mingyu Xu, Meng Li, Ying Zhang, Huitu Zhang)....Pages 155-163
 Optimization of Processing Parameters of Stabilizers After Enzymes Hydrolysis for Cloudy Ginkgo Juice  (Haifeng Yu, Junyan Liu, Jingxi Yang)....Pages 165-173
 Characterization of Recombinant Dioxygenase from Bacillus thuringiensis  (Ning Xue, Zhixiang Li, Lei Zhao, Jie Ma, Qingyang Xu, Chenglin Zhang et al.)....Pages 175-182
 Optimization of Condition for Recombinant l-Isoleucine Dioxygenase Expression in Escherich coli BL21(DE3)  (Jie Ma, Zhixiang Li, Lei Zhao, Qingyang Xu, Chenlin Zhang, Xixian Xie et al.)....Pages 183-190
 Protein Sparing Effect of Carbohydrate on Growth Performance, Digestion Ability of Common Carp (Cyprinus carpio) at Different Feeding Frequencies  (Ze Fan, Xiuting Qiao, Jinhui Sun, Pei Cui, Zhenzhen Fang, Dongqing Bai et al.)....Pages 191-202
 Engineering of Industrial Aspergillus ochraceus Strains for Improved Steroid 11α-Hydroxylation Efficiency via Overexpression of the 11α-Hydroxylase Gene CYP68J5  (Xingwei Yang, Fan Wu, Xiangjiang Hou, Benfeng Lin, Ruijie Wang, Fuping Lu et al.)....Pages 203-212
 Response Surface Methodology Optimization Extraction of Polysaccharides from Maca (Lepidium meyenii) Leaves and Primary Characterization  (Caicai Kang, Liming Zhang, Limin Hao, Huanhuan Ge, Meng Xu, Jie Cao et al.)....Pages 213-224
 Metabolomic Analysis of Dunaliella salina upon Concurrent Deprivation of Nitrogen and Phosphor  (Hexin Lv, Xianggan Cui, Shilei Wang, Shiru Jia)....Pages 225-233
 Effect of Yeast Extract on Production of ε-poly-L-lysine by Streptomyces diastatochromogenes  (Fengzhu Guo, Haoran Zheng, Xue Zhang, Yawen Cheng, Zhilei Tan, Shiru Jia)....Pages 235-244
 Comparison of Aroma Compounds in Sauce-Flavor and Sesame-Flavor “Shan Zhuang Lao Jiu” Liquors by Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry  (Fei Liu, Wanqiang Yin, Liping Du, Dongguang Xiao)....Pages 245-254
 Optimization the Fermentation Conditions of Marasmius androsaceus by Desirability Function Method  (Jia Song, Le Cui, Xiaobo Ma, Yan Su, Zhengmei Huang, Min Wang)....Pages 255-265
 Heterologous Expression and Enzyme Properties of β-mannanase from Trichoderma reesei in Pichia pastoris  (Qing Ma, Lijuan Ma, Rui Cai, Fengchao Jiang, Pan Song, Dongguang Xiao)....Pages 267-275
 Reduction of Characteristic Biogenic Amines Production by Synergistic Fermentation of Salt-Tolerant Yeast in Soy Sauce  (Wei Qi, Wen-Tao Zhang, Fu-Ping Lu)....Pages 277-287
 Effect of Temperature, NaCl and Ferulic Acid Concentration on Bioconversion of Ferulic Acid to 4-Vinylguaiacol and 4-Ethylguaiacol by Halotolerant Yeasts Candida versatilis  (Wei Qi, Wen-Tao Zhang, Fu-Ping Lu)....Pages 289-297
 Near Infrared Spectroscopic (NIRS) Analysis of Polysaccharides and Ergosterol Contents in Tricholoma matsutake Mycelium by Improved Chemometric Model  (Qiubo Chu, Quan Li, Shuang Hu, Xue Jiang, Yanzhen Wang, Hao Zeng et al.)....Pages 299-307
 Antioxidant Activity and Hepatoprotective Activity of Shanxi Aged Vinegar in Hydrogen Peroxide-Treated HepG-2 Cells  (Ting Xia, Jiahui Yao, Jiankang Wang, Jin Zhang, Min Wang)....Pages 309-316
 Analysis and Application of the Promotion Action of Methanol on Sorangium cellulosum Produce Epothilones  (Lin-hao Zhang, Lin Zhao, Xin Sun, Xin-li Liu)....Pages 317-327
 Optimization of Solid Fermentation Process of Bacillus megaterium and Its Application in Crop Growth  (Jinzhao Liu, Lin Zhao, Dong Ma, Xin Sun, Xin-li Liu)....Pages 329-338
 Comparative Quantitative Analysis of Probiotic Bacteria During Puer Tea Pile Fermentation  (Shuang Li, Zhongyuan Li, Cuixia Feng, Tongcun Zhang)....Pages 339-348
 Prediction of Hot Spots in Dimer Interface of Green Fluorescent Protein  (Wenjuan Zhang, Lin Wang, Zhiwei Sun, Bianqiang Zhang, Qiaoqiao Tang, Qiang Gao)....Pages 349-355
 Regulation of NAD (H) Pool by Overexpression of Nicotinic Acid Phosphoribosyltransferase for AD (D) Production in Mycobacterium neoaurum  (Liqiu Su, Yanbing Shen, Tian Gao, Le Cui, Jianmei Luo, Min Wang)....Pages 357-364
 Study on the Different Replacing Groups of Trans-Paroxol for Enzymatic Resolution Using Molecular Simulations  (Chuan Zhang, Yigang Jia, Chao Xu, He Huang, Yi Hu)....Pages 365-374
 Relationship Between Coenzyme Q10 Synthesis and Cytochrome Accumulation in Rhodobacter sphaeroides 2.4.1  (Penghao Li, Dan Gao, Junqian Gao, Hao Liu, Zhengliang Qi)....Pages 375-384
 Optimization of the One-Step and Two-Step Transformation Methods of Mannitol by Lactobacillus buchneri  (Hongbin Wang, Yu Li, Yongshuai Wang, Yan Chen, Yong Zhang, Yongrui Feng et al.)....Pages 385-392
 Optimization of Electroporation Conditions for Arthrobacter simplex  (Jianmei Luo, Haijie Xue, Shengnan Zhao, Xiangsheng Liu, Xiangrong Chen, Jiajia Liu et al.)....Pages 393-402
 Medium Optimization for γ-Aminobutyric Acid Production by Response Surface Methodology  (Chuan-You Chang, Shen-Xi Ma, Jun Zhang, Qiang Gao)....Pages 403-412
 A Concise and Diversity-Oriented Strategy for the Synthesis of Substituted 2-Pyrrolidinones via an Ugi/Intramolecular Inverse Electron Demand Diels-Alder Sequence  (Yan Liu, Tianyi Shang, Zhong Chen, Yaozhou Zhang, Wei Sun, Junjie Yang et al.)....Pages 413-419
 Optimization of Medium Components for the Production of Flavonoids and Soluble Protein with Phellinus igniarius in Liquid Culture  (Yingchao Wang, Zhouli Yuan, Xinrong Tan, Zaohong Ran, Hong Jin)....Pages 421-431
 Application of Sun Light Conversion Film on the Outdoor Culture of Nostoc flagelliforme  (Shigang Shen, Peipei Han, Shunyu Yao, Rongjun Guo, Shiru Jia)....Pages 433-439
 Determination of Ginkgolic Acids in Ginkgo Seeds Using HPLC-MS in the Presence of Lipids  (Yanying Hu, Guojuan Sun, Huitu Zhang, Liming Zhang, Tongcun Zhang, Yujie Dai)....Pages 441-450
 High Concentration Vinegar Production by Acetic Acid Bacteria Using Edible Alcohol as Feedstock  (Xiao-Yan Yin, Rong Zhai, Wu-Kun Zhong, Jiao Huo, Zhong-Hua Yang)....Pages 451-458
 Optimization of Process for Optical Resolution of dl-Pantolactone Using Immobilized d-Lactonohydrolase  (Mei-juan Xuan, Jian-zhong Zhang, Hao-yu Yuan, Run Wei, Fu-ping Lu, Ming Li)....Pages 459-467
 Enzymatic Synthesis of l-Cysteine by Escherichia coli Whole-Cell Biocatalyst  (Mingli Ma, Tao Liu, Heyun Wu, Fangqing Yan, Ning Chen, Xixian Xie)....Pages 469-478
 Analysis on Acid, Bile, and Heat Tolerance of Probiotics Strains in Maca-Probiotics Granule  (Ya-Xin Jiang, Qing-Qing Dong, Ji-Ping Qu, Tong-Cun Zhang, Ya-Jian Song, Zhong-Yuan Li et al.)....Pages 479-485
 Near-Infrared Spectroscopy for the Monitoring of Leucine Fermentation in Corynebacterium glutamicum  (Hongbo Wei, Tuo Shi, Lihong Du, Qixin Chen, Yuechao Ma, Quanwei Zhang et al.)....Pages 487-494
 Modification of Corynebacterium glutamicum YILW for Isoleucine Production Improvement  (Ning Xue, Zhixiang Li, Junjie Zhan, Jie Ma, Qingyang Xu, Chenglin Zhang et al.)....Pages 495-504
 Liquid-Liquid Extraction of Hydrocortisone from the Fermentation Liquor Contain Hydroxropyl-β-Cyclodextrin  (Dongchao Yuan, Yanling Dong, Yanbing Shen, Qing Zhao, Min Wang)....Pages 505-511
 Conversion of Food Waste and Feldspar into Biofertilizer Using a Stress-Tolerant Keldspar-Solubilizing Bacillus Subtilis Xue-113168  (Shengping Xue, Liangtian Miao, Jinjun Xue, Hanbo Yan, Guiqin Li)....Pages 513-522
Front Matter ....Pages 523-523
 Biological Degradation of Aflatoxin B1 by Emericellopsis sp. 1912 and Sarocladium sp. 10A  (Hui Wang, Zhongyuan Li, Haiyan Qiu, Shuang Li, Tongcun Zhang)....Pages 525-532
 Study on Ultrasonic-Assisted Extraction of Star Anise Oleoresin from the Fruits of Illicium verum and Preliminary Investigation of Its Antimicrobial Activity  (Ping Li, Zhan Shu, Lan Zhang, Tao Li, Lin Tian)....Pages 533-544
 Preparation of Dialdehyde Cellulose and Its Antibacterial Activity  (Huanhuan Ge, Liming Zhang, Meng Xu, Jie Cao, Caicai Kang)....Pages 545-553
 Optimization of Microwave-Assisted Extraction of Total Flavonoids from China-Hemp Leaves and Evaluation of Its Antioxidant Activities  (Jie Cao, Limin Hao, Liming Zhang, Meng Xu, Huanhuan Ge, Caicai Kang et al.)....Pages 555-567
 Comparison of Different Protocols of Gradient Ammonium Sulfate Fractionation of Antibacterial Proteins/Peptides from Clarias gariepinus Wastes  (Xiaomei Wang, Yan Wang, Yunxia Xu, Zhuanzhuan Li, Chengxun Chen, Jinwei Gao et al.)....Pages 569-577
 Screening of Microbial with the Ability of Epothilones Biotransformation  (Meng Zhang, Lin Zhao, Xin Sun, Xin-li Liu)....Pages 579-586
 Isolation, Screening and Evaluation of Potential Biocontrol Endophytes Against Ralstonia solanacearum on Ginger  (Ning Zhou, Lin Zhao, Xin Sun, Xin-li Liu)....Pages 587-596
 Isolation and Molecular Identification of Lactobacillus brevis from Spoilage Craft Beer in China  (Zhu Liping)....Pages 597-603
 Production of 5′-Inosinic Acid by Whole-Cell Biocatalyst Expressing a Mutated Acid Phosphatase/Phosphotransferase  (Hui Yuan, Zi-fan Jia, Ju-hua He, Xiao-guang Fan, Ning Chen)....Pages 605-614
 Bio-production of L-rhamnonate by Pseudomonas taetrolens  (Shuhong Mao, Jianlin Wu, Lixia Zhang, Shuqi Gui, Fuping Lu)....Pages 615-621
 Preparation and Stability Evaluation of Direct Vat Set Lactobacillus helveticus AAF1-5  (Chuanxue Fu, Shuai Yang, Jun Mou, Jia Song, Min Wang, Yu Zheng)....Pages 623-629
 Overexpression of the Endo-inulinase Gene from Two Different Sources and Characteristics Analysis  (Li-Kun Wei, Qing-Long Xin, Zhi-Mei Feng, Xue-Yan Xing, Wei Feng, Yu Li)....Pages 631-642
 Production of Ethyl Acetate Catalyzed by Activated Carbon-Based Solid Acid Catalyst  (Jia Li, Yan Li, Hua Zhao)....Pages 643-651
 The Preparation of Chitosan from Corncob Hydrolyzate by Actinomucor elegans  (Yan Li, Jia Li, Hua Zhao)....Pages 653-662
 High-Efficiency Separation and Purification of Taq DNA Polymerase  (Hao Zhou, Yujie Zhang, Zhiyin Hu, Ai Mu, Xiangchao Gu)....Pages 663-672
 Determination of Activity and Extraction of Thrombin from the Porcine Blood  (Tianjun Li, Heng Li, Hong Pan, Tao Li, Jun Shi)....Pages 673-680
 An Efficient Method for Isolation and Separation of Pigments from Streptomyces alboflavus TD-1  (Xiaoyue Gu, Yali Zhang, Laifeng Lu, Zhenjing Li, Changlu Wang)....Pages 681-691
Front Matter ....Pages 693-693
 Establishment of the Method for Screening Small Molecule Inhibitors Blocking the Interaction Between PD-1 and Its Ligand PD-L1  (Lei Jing, Fushan Yan, Yingchun Wang, Bo Jiang, Li Chang, Cheng Cheng et al.)....Pages 695-703
 Construction of the PD-L1 Promoter-Luciferase Reporter Expressing Vector for Small Molecule Inhibitors Screening  (Bo Jiang, Zhichen Shi, Ali Wang, Yuyin Li, Qiurong Zhang, Lei Jing et al.)....Pages 705-712
 Construction and Functional Analysis of BNP Promoter Luciferase Reporter Plasmid  (Jian Zhang, Nan Wang, Yanzhong Liu, Man Li, Huiqin Gong, Hongpeng He et al.)....Pages 713-718
 HPV18 E6 and E7 Influence the Expression of Cancer Related LncRNAs in HeLa Cells  (Xiang Liu, Yongwei Lai, Hailin Yao, Mengmeng Zhang, Hao Zhou, Tongcun Zhang et al.)....Pages 719-727
 Construction of Tip60-Encoding Plasmid and the Effect of Tip60 on the Expression of HPV18 Genes in HeLa Cells  (Yongwei Lai, Xuena Liu, Yijie Wang, Yunpeng Yue, Xiang Liu, Hao Zhou et al.)....Pages 729-735
 Investigation of Aquatic Pathogens and Diversity Analysis of Aeromonas Isolates  (Zhaoyuan Jing, Yang He, Qian Li, Bo Zhang, Hongjiang Yang)....Pages 737-744
 Expression of Transcription Factor EB (TFEB) Promotes Cancer Cell Proliferation, Migration and Invasion  (Wei Li, Yang Liu, Min Hao, Meng Yang, Shuang Zhao, Zhenxing Liu et al.)....Pages 745-753
 Research Progress of Squalene Synthase on Function and Application  (Dengyue Sun, Qianqian Guo, Zhangliang Zhu, Songtao Li, Jian-Wen Wang, Yu-Fu Zhang et al.)....Pages 755-765
 Review in Metabolic Modulation of Higher Alcohols in Top-Fermenting Yeast  (Zhongguan Sun, Dongguang Xiao)....Pages 767-773
 Research Progress of Aldehyde Ketone Reductase for Asymmetric Catalysis of Chiral Compounds  (Songtao Li, Zhangliang Zhu, Jian-Wen Wang, Qianqian Guo, Panpan Xu, Dengyue Sun et al.)....Pages 775-781
 Population Structure and Genetic Diversity Analysis of Peanut (Arachis hypogaea L.) Using Molecular Markers  (Xiu Rong Zhang, Feng Zhen Liu, Kun Zhang, Yong Shan Wan)....Pages 783-793
 Research on γ-Polyglutamic Acid Fermentation with Extract from Waste Beer Yeast  (Jun Yu, Lin Zhao, Song Li, Xin Sun, Xin-li Liu)....Pages 795-801
 Isolation and 16SrDNA Identification of Bacteria from Traditional Kazak Dairy Products  (Wenyuan Sun, Yanli Fan, Jing Li, Gaoshaer Kayierhali, Xuejiao Liu, Yun Hao et al.)....Pages 803-809
 Research on Extracting Technology of Chlorogenic Acid from Honeysuckle  (Yang Sun, Ye-Min Yu, Hong-Bo Suo, Ying-Lan Zhu, He Huang, Yi Hu)....Pages 811-822
 Construction and Functional Analysis of Luciferase Reporter Plasmid Containing Vimentin Gene Promoter  (Cheng-Xi Yu, Yuan Xiang, Xing-Hua Liao, Xiao-Yu Zhang, Hui Li, Jia-Peng Li et al.)....Pages 823-828
 STAT5A and MKL-1 Activate the Activity of Luciferase Reporter Plasmid Containing FOXP3 Gene Promoter  (Jia-Peng Li, Hui Li, Xiao-Yu Zhang, Cheng-Xi Yu, Yuan Xiang, Ze Yin et al.)....Pages 829-837
 Study the Role of E-selectin and Its Ligand sLeX in the Adhesion Between THP-1 Cells and HUVEC Cells  (Qian Zhang, Huan Liu, Chaoran Yao, Tingshen Li, Xuehui Li, Li Zhang et al.)....Pages 839-846
 The Application of Microbial Technology in Harbor Engineering: The Impact of Extracellular Polymeric Substances on the Sedimentation and Properties of Fluid Mud  (Xiaohua Chen, Qixiu Pang, Mengnan Li, Baojiang Sun, Ruibo Zhang, Shiru Jia et al.)....Pages 847-857
 Polyvalent Effect Enhances Anti-influenza Virus Activity  (Haipeng Liu, Haojie He, Zhaoliang Yang, Peng Yu, Kui Lu)....Pages 859-865